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Application of combination of FAM83A, KPNA2, KRT6A and LDHA as lung adenocarcinoma biomarker

A lung adenocarcinoma and marker technology, applied in the field of biomedicine, can solve the problems of low sensitivity and specificity, difficult diagnosis and evaluation of lung adenocarcinoma, false positives, etc., to facilitate popularization and application, improve sensitivity and specificity, Result easy effect

Active Publication Date: 2022-02-11
SHANGHAI BIOCHIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is difficult to accurately and specifically diagnose and evaluate lung adenocarcinoma relying on a single marker
The markers currently used for the diagnosis of lung adenocarcinoma have problems of low sensitivity and specificity, which can easily cause false positive or false negative results, and cannot be well applied to the diagnosis of lung adenocarcinoma or to predict the survival of lung cancer or the personality of lung cancer Chemotherapy

Method used

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  • Application of combination of FAM83A, KPNA2, KRT6A and LDHA as lung adenocarcinoma biomarker
  • Application of combination of FAM83A, KPNA2, KRT6A and LDHA as lung adenocarcinoma biomarker
  • Application of combination of FAM83A, KPNA2, KRT6A and LDHA as lung adenocarcinoma biomarker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] In the data of 594 cases of lung adenocarcinoma, the differential expression of FAM83A, KPNA2, KRT6A and LDHA of specific genes was analyzed and drawn into a histogram. The results are as follows figure 1 shown by figure 1 It can be seen that the four genes are highly expressed in patients with lung adenocarcinoma, and there are significant differences (P values ​​are 1.09×10 -3 , 1.88×10 -3 , 7.98×10 -4 , 4.88×10 -4 ).

[0132] Among them, the Control group is the normal group of the TCGA database.

Embodiment 2

[0134] The cancer and paracancerous tissue samples of 8 patients clinically confirmed as lung adenocarcinoma were taken, homogenized and crushed, total RNA was extracted, reversed into cDNA, and real-time fluorescent quantitative PCR experiments of FAM83A, KPNA2, KRT6A and LDHA were designed based on the fluorescent dye method , and then detect the expression of genes FAM83A, KPNA2, KRT6A and LDHA in cancer and paracancerous tissues (such as figure 2 , wherein, Normal (Control) refers to: paracancerous sample (Paracancerous) sample number n=8; Case refers to: lung adenocarcinoma sample (Lungadenocarcinoma) sample number n=8), and the primer sequences are shown in Table 1 below. Depend on figure 2 It can be seen that compared with the paracancerous tissue, the four genes are all highly expressed in the cancer tissue, and there are significant differences.

[0135] Table 1 gene primer sequence

[0136]

Embodiment 3

[0138] Download the clinical survival data information of 522 cases of lung adenocarcinoma patient samples in the TCGA database. Based on the Kaplan-Meier method to analyze the influence of the expression levels of differential genes FAM83A, KPNA2, KRT6A and LDHA on the survival rate of patients, the results showed that patients with high expression of FAM83A, KPNA2, KRT6A and LDHA genes had significantly worse survival rates than other patients. Prognosis, and vice versa has a better prognosis. And through the p-value significance analysis, it is confirmed that the result is statistically significant (such as image 3 ,like Figure 4 ,like Figure 5 ,like Image 6 ).

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Abstract

The invention belongs to the field of biological medicine, and discloses application of a combination of FAM83A, KPNA2, KRT6A and LDHA genes as a lung adenocarcinoma biomarker. The invention also discloses an application of the substance for detecting the FAM83A, the KPNA2, the KRT6A and the LDHA in preparation of the kit. The FAM83A, the KPNA2, the KRT6A and the LDHA are used as biomarkers for joint detection, and an ideal expression profile analysis strategy and a novel combined biomarker kit for risk assessment and prognosis analysis of the lung adenocarcinoma are provided, wherein the combined biomarker kit can be effectively used for diagnosis or prognosis assessment of the lung adenocarcinoma and has a good clinical application prospect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of FAM83A, KPNA2, KRT6A and LDHA combined as lung adenocarcinoma biomarkers. Background technique [0002] Lung cancer is a malignant tumor with the highest morbidity and mortality rate in the world. Lung cancer can be divided into two categories: small cell lung cancer and non-small cell lung cancer. Non-small cell lung cancer accounts for about 85% of lung cancer, most of which are non-small cell lung cancer. All belong to lung adenocarcinoma (LUAD), and its five-year survival rate is low. [0003] Although a combination of surgery, radiochemotherapy, targeted therapy, and immunotherapy is used, the prognosis of LUAD remains poor due to local recurrence or distant metastasis. The pathogenesis and prognosis of lung adenocarcinoma are complex, involving multiple genes and environmental factors. Therefore, it is difficult to accurately and specifically diagnose and evaluate lu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/11
CPCC12Q1/6886C12Q1/686C12Q2600/158C12Q2600/118C12Q2600/112C12Q2545/114Y02A50/30
Inventor 产天龙徐祎春韩峻松袁箐苏军周佳菁
Owner SHANGHAI BIOCHIP