Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium

A solid culture medium and detection device technology, applied in sampling devices, analysis materials, instruments, etc., can solve the problems of sample waste, inability to guarantee aseptic operation, difficulty in sampling and detection at the same time, etc., to eliminate the probability of bacterial contamination, Good sterilization effect, guaranteed drying effect

Pending Publication Date: 2022-03-01
CHANGCHUN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the limited detection technical conditions, it is difficult to carry out sampling and detection of microbial suspension and solid medium at the same time. The sampling process basically relies on manual labor, and mechanical automation is rarely used for sampling. After manual sampling, sam

Method used

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  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium
  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium
  • Intelligent sampling and detecting device suitable for microorganism suspension liquid and solid culture medium

Examples

Experimental program
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Example Embodiment

[0030] Example 1

Example Embodiment

[0033] Example 2

[0034] Detailed embodiments from the flat petri dish Tieniji spore:

[0035] When the adaptive sampling brush head (13) is perpendicular to the multi-region sample disk (7) direction, the spore sampling in the solid flat petri dish, the adaptive brush head (13) is maintained for 3 seconds with spore colonies, and the sample is completed The adaptive sampling brush head (13) adsorbed with spores was transferred to the dilution of the dilution system for the dilution of spore suspension. The multi-region sample disc is sterilized with anhydrous ethanol, and the sampling period is 0-8 days, the sampling time is 10:00 per day, and when the adaptive sampling brush is perpendicular to the multi-zone sample disk, for solid Spore sampling in flat plate culture dishes, the intelligent detection device inspects whether Tamani is stunned by the cefosporoscopic culture dish, and if the styles are sent to the stereo sterilization, there is no styles to carry out Raman is col...

Example Embodiment

[0036] Example 3

[0037] Specific embodiments of taking threto yeast from a bevel culture:

[0038] The multi-region sample disc is sterilized with anhydrous ethanol, and the sampling period is 0-5 days, the sampling time is 10:00 per day, and the inclined medium is placed on a multi-region sample disk, and the inclined medium The height of the multi-zone sample disc is 5 cm, and the intelligent detection device inspects whether the thies of the thies of the thies, if the pyctonia is sent to the stereo sterilization, there is no stolen yeast inclined culture film to collect, According to 45 °, the sampling point is set, the robot arm controls the sampling needle tilt 45 ° parallel to the tube direction; the sample needle extends into the test tube to gently scrape the colony, and the sample is placed in the sample, which will belt from the spore yeast Adapting to the sampling brush is sent to the dilution system to dilute with physiological saline, making the spore yeast suspensi...

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Abstract

The invention discloses an intelligent sampling and detecting device suitable for microorganism suspension liquid and a solid culture medium. The device comprises an intelligent sample detecting system, a three-dimensional sterilizing bin, a multi-dimensional sampling period controller, a synchronous sampling controller, an image collecting system, a strain recognizing device, a sample diluting system and an information storing system. The method comprises the following steps: detecting whether a sample is contaminated by bacteria or not through a sample intelligent detection system, identifying a strain of a non-contaminated sample, performing image acquisition, sampling according to a set sampling period and sampling time, diluting the sample according to detection requirements, detecting the sample, and finally storing all data into an information storage system; according to the invention, traditional manual sampling can be replaced, the bacterial contamination rate in the detection process is reduced, a strain recognition system can lock a target system and eliminate infectious microbes, and the probability of bacterial contamination in the fermentation culture process is more favorably eliminated.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to an intelligent sampling and detection device suitable for microbial suspension and solid medium. Background technique [0002] Microbial fermentation and cultivation technology is a very important research direction at present. In the process of microbial fermentation and cultivation, it involves the growth and metabolism of bacteria, as well as the influence of different fermentation conditions on microbial fermentation. The parameters of the product are measured, and the measurement results are of great significance to the research results. [0003] Due to the limited detection technical conditions, it is difficult to carry out sampling and detection of microbial suspension and solid medium at the same time. The sampling process basically relies on manual labor, and mechanical automation is rarely used for sampling. After manual sampling, sample testing needs to be carried...

Claims

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Application Information

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IPC IPC(8): G01N1/14
CPCG01N1/14G01N35/10
Inventor 刘欣孔燕琴杨紫琪王赫
Owner CHANGCHUN UNIV OF TECH
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