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Myriophyllum elatinoides functional feed and preparation method thereof

A technology of green foxtail algae and functional feed, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve problems such as secondary pollution, reduce feed cost, enrich nutrients, and avoid secondary pollution. The effect of pollution

Active Publication Date: 2022-03-04
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above problems, the object of the present invention is to provide a method for preparing a viridiflorum functional feed, and the viridiflorium functional feed produced by the method improves the palatability of the viridiflorium feed, contributes to Maintain the balance of animal intestinal flora, promote the production of beneficial metabolites, and at the same time solve the problem of secondary pollution caused by the treatment of waste water by P. viridis, and realize the mutual unity of environmental protection and resource development and utilization

Method used

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  • Myriophyllum elatinoides functional feed and preparation method thereof
  • Myriophyllum elatinoides functional feed and preparation method thereof
  • Myriophyllum elatinoides functional feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Probiotic properties of Lactobacillus plantarum BW2013

[0067] 1. In vitro simulated gastrointestinal digestion experiment

[0068] 1) Preparation of artificial saliva

[0069] 6.2g NaCl, 2.2g KCl, 0.22g CaCl 2 , 1.2 g NaHCO 3 Dissolve in 1L deionized water and autoclave. Cool to about 20°C, add 3.0g / L α-amylase, adjust the pH to 6.9, and filter through a 0.22 micron microporous membrane to sterilize.

[0070] 2) Preparation of artificial gastric juice

[0071] 3.0g NaCl, 1.1g KCl, 0.15g CaCl 2 , 0.60g NaHCO 3 Prepare into 1L solution and sterilize by high-pressure steam; cool to about 20°C and add 3.0g / L pepsin obtained from pig gastric mucosa, adjust pH to 2.0 with 10N HCl, and filter to sterilize with 0.22 micron microporous membrane.

[0072] 3) Preparation of artificial small intestine fluid

[0073] 5.0g NaCl, 0.60g KCl, 0.30g CaCl 2 , 0.60g NaHCO 3 Dissolve in 1L deionized water and autoclave. Cool to about 20°C, add 3.0g / L ox bile, 1.0g / L ...

Embodiment 2

[0081] The probiotic effect of embodiment 2 Lactobacillus plantarum BW2013

[0082] 1. Preparation of DSS: prepare a dextran sodium sulfate solution with a mass fraction of 2.5%, and the solvent is phosphate buffer saline.

[0083] 2. Preparation of live bacterial suspension of Lactobacillus plantarum BW2013: inoculate Lactobacillus plantarum BW2013 in 500ml of MRS liquid medium according to the inoculum size of 10% (w / w), culture at 37°C for 18h, and incubate at 8000r / min The bacterial cells were collected by centrifugation for 15 min; 8 CFU / ml low-dose bacterial suspension L (DL group), 2.34×10 9 Medium-dose bacterial suspension M (DM group) of CFU / ml, 2.34×10 10 CFU / ml high-dose bacterial suspension H (DH group), NC is the normal control group (sterile PBS), D group is the model group successfully established with DSS, and PC is the mesalazine positive control group.

[0084] 3. Modeling: 72 BALB / c mice were fed with Lactobacillus-free mouse feed for one week and then ra...

Embodiment 3

[0118] Embodiment 3: preparation of viridiflora functional feed

[0119] 1. Feed preparation steps

[0120] 1) Raw material cutting

[0121] Clean the harvested fresh Myriophyllum aquaticum to ensure that the samples are free from mildew and deterioration. The washed samples are dried to reduce their water content to 55-65%, and then cut into 1±0.5cm short paragraph.

[0122] 2) Inoculate the mixture

[0123] Will 1×10 9 The bacterium solution of Lactobacillus plantarum BW2013 of CFU / mL concentration is evenly sprayed on the air-dried and cut Prunus viridifera at an inoculum size of 10% (w / w), and then 6% (w / w) of sucrose solid is added, Mix well to make a mix.

[0124] 3) Bagging and sealing

[0125] Put the mixture into a sterile plastic bag, use a small vacuum sealer to vent the air and seal it.

[0126] Put the bagging amount of 500g of the mixture into a sterile plastic bag, use a small vacuum to exhaust the air, and seal it. The amount of bagging is determined ac...

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Abstract

The invention provides a preparation method of myriophyllum elatinoides functional feed. The preparation method comprises the following steps: 1) cutting raw materials; (2) inoculating and mixing; (3) bagging and sealing; 4) performing constant-temperature fermentation; 5) drying to prepare powder; and 6) mixing viable bacteria. The prepared myriophyllum elatinoides functional feed is fermented by adopting lactobacillus plantarum, so that the protein content, fat content and ash content in the myriophyllum elatinoides are increased, and the cellulose content in the myriophyllum elatinoides is reduced. According to the feed, the nutritional value and palatability of the myriophyllum elatinoides feed are improved, and the feed is convenient to store and transport; the intestinal flora structure of animals is improved, the production of beneficial metabolites is promoted, and 10%-20% of conventional feed can be replaced; meanwhile, the problem that secondary pollution is easily caused after the culture wastewater is treated by the myriophyllum verticillatum is solved, and mutual unification of environmental protection and resource development and utilization is realized.

Description

technical field [0001] The invention belongs to the technical field of feed processing, and relates to a functional feed for viridiflora and a preparation method thereof. Background technique [0002] In recent years, the global food crisis has intensified, and the international food price has risen rapidly. my country's feed grain accounts for 1 / 3 of the total grain output, and 40.0% of the cultivated land is used for feed production. With the rapid development of my country's animal husbandry industry, protein and energy feed resources are in short supply, and the cost of breeding is too high. The shortage of feed resources has always affected the sustainable development of animal husbandry and has become a major challenge for my country's animal husbandry. The development and utilization of grain and feed is an urgent problem to be solved in the current aquaculture development. [0003] At the same time, with the vigorous development of my country's aquaculture industry, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/12A23K20/163
CPCA23K10/30A23K10/12A23K20/163A23V2400/169Y02P60/87
Inventor 李祖明
Owner BEIJING UNION UNIVERSITY
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