Fluorescent staining slide preparation method for observing forms of glandular hair and non-glandular hair of plant leaf epidermal hair

A technology of fluorescent dyeing and epidermis, applied in the field of fluorescent dyeing film production, to achieve the effect of simple operation, high success rate and low technical requirements

Pending Publication Date: 2022-03-04
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For above-mentioned situation, in order to overcome the defective of prior art, the purpose of the present invention is exactly to provide a kind of method for making a film of fluorescent staining that is used to observe plant leaf epidermis glandular trichome and non-glandular hair form, can effectively solve the problem of using fluorescence microscope to plant. The problem of observation of epidermal hair

Method used

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  • Fluorescent staining slide preparation method for observing forms of glandular hair and non-glandular hair of plant leaf epidermal hair
  • Fluorescent staining slide preparation method for observing forms of glandular hair and non-glandular hair of plant leaf epidermal hair
  • Fluorescent staining slide preparation method for observing forms of glandular hair and non-glandular hair of plant leaf epidermal hair

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Embodiment 1

[0022] The present invention is a kind of fluorescent dyeing method for observing the shape of glandular and non-glandular hairs of plant leaf epidermis, comprising the following steps:

[0023] (1) Leaf selection: take leaves of fresh and healthy plants, ultrasonically clean them in distilled water for 5.5 minutes, and the ultrasonic frequency is 25KHz;

[0024] (2) Immersion treatment: cut 9mm small pieces from the leaves after ultrasonic cleaning, and immerse them in a mixture of 4mL alcohol and glacial acetic acid for 2h;

[0025] (3) Rinsing: Take out the leaves after immersion treatment in step (2), and rinse them with distilled water for 2 minutes;

[0026] (4) Stand still: put the rinsed leaves into 4mL of phloroglucinol solution, let stand for 20min, the mass content of phloroglucinol solution is 7.5%;

[0027] (5) Observation: Take out the leaves from the phloroglucinol solution, place the hairy side up on a glass slide, add a drop of 25% dilute glycerin (about 0.02...

Embodiment 2

[0029] The present invention is a kind of fluorescent dyeing method for observing the shape of glandular and non-glandular hairs of plant leaf epidermis, comprising the following steps:

[0030] (1) Leaf selection: take leaves of fresh and healthy plants, ultrasonically clean them in distilled water for 4 minutes, and the ultrasonic frequency is 28KHz;

[0031] (2) Immersion treatment: cut 8mm small pieces from the leaves after ultrasonic cleaning, and immerse them in a mixture of 3mL alcohol and glacial acetic acid for 1.2h;

[0032] (3) Rinse: Take out the leaves after immersion treatment in step (2), and rinse with distilled water for 1 min;

[0033] (4) Stand still: put the rinsed leaves into 3mL of phloroglucinol solution, let stand for 30min, the mass content of phloroglucinol solution is 6%;

[0034] (5) Observation: Take out the leaves from the phloroglucinol solution, place the hairy side up on a glass slide, add a drop of 22% dilute glycerin (about 0.02mL) on the to...

Embodiment 3

[0036] The present invention is a kind of fluorescent dyeing method for observing the shape of glandular hairs and non-glandular hairs of plant leaf epidermis, comprising the following steps:

[0037] (1) Leaf selection: take leaves of fresh and healthy plants, ultrasonically clean them in distilled water for 8 minutes, and the ultrasonic frequency is 20KHz;

[0038] (2) Immersion treatment: cut 10mm small pieces from the leaves after ultrasonic cleaning, and immerse them in a mixture of 5mL alcohol and glacial acetic acid for 2.8h;

[0039] (3) Rinsing: Take out the leaves after immersion treatment in step (2), and rinse them with distilled water for 3 minutes;

[0040] (4) Stand still: put the rinsed leaves into 5mL of phloroglucinol solution, let stand for 10min, the mass content of phloroglucinol solution is 9%;

[0041] (5) Observation: Take out the leaves from the phloroglucinol solution, place the hairy side up on a glass slide, add a drop of 28% dilute glycerin (about...

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Abstract

The invention relates to a fluorescent staining slide preparation method for observing glandular hair and non-glandular hair forms of a plant leaf epidermal hair cover, which can effectively solve the problem of observing the plant epidermal hair cover by using a fluorescence microscope, and comprises the following steps of: ultrasonically cleaning leaves of a fresh and healthy plant by using distilled water, cutting into small blocks, and immersing in a mixed solution of alcohol and glacial acetic acid; the method comprises the following steps of: rinsing with distilled water, standing in a phloroglucinol solution, taking out a leaf, putting the leaf on a glass slide with one surface with epidermal hair upward, dropwise adding a drop of dilute glycerol above the leaf, respectively exciting with blue light or green light under a fluorescence microscopy, observing that glandular hair and non-glandular hair emit bright yellow fluorescence, and determining that the glandular hair and non-glandular hair emit yellow fluorescence. The fluorescent staining sheet for observing the forms of the glandular hair and the non-glandular hair of the plant leaf epidermis is obtained. The method disclosed by the invention is scientific and reasonable, simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect, and is an innovation of a fluorescent staining slide preparation method for microscopically observing plant leaf epidermal hair glandular hair and non-glandular hair forms of folium artemisiae argyi and artemisia scoparia.

Description

technical field [0001] The invention relates to the technical field of plant structure, in particular to a method for observing the glandular and non-glandular hairs of plant leaf epidermis by using a fluorescent microscope to observe the morphological characteristics of plant leaf epidermis by performing fluorescent dyeing treatment on the plant surface. Morphological fluorescent staining preparation method. Background technique [0002] The epidermal trichomes of plants are a specialized structure on the surface of plants. Epidermal trichomes are usually distributed on the leaves, stems or perianth sheets of plants. They have various shapes and can be divided into glandular hairs and non-glandular hairs according to whether they have secretion ability. Glandular hairs; according to the composition of cells, it can be divided into unicellular hairs and multicellular hairs, single row of hairs and multiple rows of hairs; according to the shape, it can be divided into head sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 郭涛魏文君冯书营回成程高富陈随清刘孟奇欧阳臻周慧
Owner HENAN UNIV OF CHINESE MEDICINE
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