Microscopic slide preparation method for observing characteristics of lower epidermis of folium artemisiae argyi

Abstract

The invention relates to a microscopic slide preparation method for observing the characteristics of the lower epidermis of folium artemisiae argyi, which effectively solves the problem of observing the pore of the lower epidermis of folium artemisiae argyi, the vertical peripheral wall pattern of epidermis cells and the distribution of glandular hair and non-glandular hair by using an optical microscope. Rinsing with distilled water, soaking with a mixed solution of sodium hydroxide and hydrogen peroxide, dissociating mesophyll tissues, bleaching, transferring into alcohol, and removing non-glandular hairs on leaf epidermis; the preparation method comprises the following steps of: adding the raw materials into a mixed solution of chromic acid and nitric acid, standing, dissociating mesophyll tissues, rinsing by using alcohol, separating upper and lower epidermis, removing mesophyll tissues of inner epidermis, dyeing the upper and lower epidermis by using a toluidine blue O solution, and rinsing by using water to remove floating color and water seal sheets, thereby obtaining the high-purity mesophyll tissue. The preparation method is simple to operate, low in cost, small in workload, high in success rate, clear in observation and good in effect; the technical problem of observing the lower epidermis of the folium artemisiae argyi is solved, the technical requirement on operators is not high, and an ideal effect can be achieved.

Description

technical field [0001] The invention relates to the technical field of microscopic identification and observation of plants, in particular to a microscopic film-making method for observing the characteristics of the lower epidermis of the leaves of Artemisia argyi, which can be used to observe and study the characteristics of the leaf epidermis by processing the leaves. Background technique [0002] Artemisia argyi is a commonly used traditional Chinese medicine, and it is produced in most parts of the country. Artemisia argyi leaves are dried and crushed to obtain "Moxa velvet", which is used to make moxa sticks for moxibustion. Glandular hairs are the main source of the volatile oil of Artemisia argyi. Therefore, the quantity and density of the glandular hairs of Artemisia argyi are related to the quality of Artemisia argyi, and the quantity and density of non-glandular hairs in Artemisia argyi are directly related to the yield and quality of Artemisia argyi. The main sour...

AI Technical Summary

Problems solved by technology

The upper epidermis of Artemisia argyi is distributed with glandular hairs and sparse non-glandular hairs, and it is easier to observe the upper epidermis with a scanning electron microscope; while the lower epidermis is densely covered with non-glandular hairs and covers the glandular hairs, stomata and epidermal cells distributed under it, so using Scanning electron microscope pairs can also only see dense nonglandular hairs

Observing the characteristics of the leaf epidermis with an optical microscope usually adopts the method of tearing the epidermis, dissociation of the epidermis, and transparent filming. However, because the leaves of Artemisia argyi are papery leaves, the leaves are thin, and the upper and lower epidermis are densely covered with glandular hairs. It is located in a depression, so it is difficult to tear off the epidermis and dissociate the epidermis. Even if the epidermis can be successfully separated, the lower epidermis is densely covered with non-glandular hairs, and characteristics such as stomata and epidermal cells cannot be observed under an optical microscope. The microscopic features of the lower epidermis cannot be seen clearly due to the dense non-glandular hairs on the lower epidermis of Artemisia argyi leaves

Due to the constraints of existing film-making techniques, it is currently difficult to observe microscopic features such as the stomata of the lower epidermis of Artemisia argyi leaves, the pattern of the anticlinal wall of epidermal cells, and the distribution of glandular and non-glandular hairs by scanning electron microscope or optical microscope. Therefore, So far, the research on the characteristics of the lower epidermis of Artemisia argyi is not deep enough

Therefore, it is very necessary to design a special microscopic preparation method for the characteristics of the lower epidermis of Artemisia argyi, to observe the characteristics of the lower epidermis of Artemisia argyi, but there is no public report so far

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