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Macroporous hydrogel 3D printing device and method

A 3D printing and hydrogel technology, applied in the field of microfluidics, can solve the problems of single structure and uncontrollability, and achieve the effect of precise control and strong editability.

Active Publication Date: 2022-03-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the cell structure in macroporous hydrogels is still uncontrollable, and the structure is single, which has become the main restricting factor for its wider application.

Method used

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  • Macroporous hydrogel 3D printing device and method
  • Macroporous hydrogel 3D printing device and method
  • Macroporous hydrogel 3D printing device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Macroporous hydrogel 3D printing device suitable for thermosensitive hydrogel one

[0071] Such as figure 1 As shown, the device includes a gas injection pump 1, an air pressure gauge 2, a T-shaped channel 3, an inlet capillary glass tube 4, an outlet capillary glass tube 5, a horizontal moving support 6, a water bath casing 7, a liquid injection pump 8, and a flow meter 9. 3D printing nozzle 10, printing bottom plate 12, submersible pump 13 and constant temperature water bath heating device 14.

[0072] The inlet capillary glass tube 4 and the outlet capillary glass tube 5 are both nested in the T-shaped channel 3, and the upper end of the inlet capillary glass tube 4 is fixedly connected to the T-shaped channel 3 by light-curing glue, and the upper end of the outlet capillary glass tube 5 Nested and fixed at the lower end of the T-shaped channel 3, a distance of 0.3-1d (d is the diameter of the capillary glass tube) is maintained between the outlet of the i...

Embodiment 2

[0087] Example 2 Macroporous hydrogel 3D printing device suitable for thermosensitive hydrogel II

[0088] Such as figure 2 As shown, compared with the device in Example 1, the lower end of the T-shaped channel 3 is directly connected to the 3D printing nozzle 10, the outlet capillary glass tube is missing, and the internal channel of the air inlet capillary glass tube 4 is rectangular in cross section, and The aspect ratio of the inner channel is greater than 10. The device adopts the principle of step emulsification to generate macroporous hydrogel, the size of the bubbles is stable, and finally can produce bubbles with uniform size and stability.

[0089] The method for printing using the device of this embodiment comprises the following steps:

[0090] (1) Configure a temperature-sensitive and ionically cross-linkable multi-component hydrogel solution as a bioprinting ink 25wt.% Plournic F127 and 1.5wt.% sodium alginate solution, add the liquid to the syringe pump 8; tu...

Embodiment 3

[0095] Example 3 Macroporous hydrogel 3D printing device suitable for photosensitive hydrogel one

[0096] Such as image 3 As shown, compared with the printing device of Example 1, this device has increased the high-energy ultraviolet light source 11 fixed on the horizontally movable support 6, and the high-energy ultraviolet light source 11 is focused on the height of the printing layer below the 3D printing nozzle 10. for cross-linking of hydrogels. In addition, the printing bottom plate 12 may also not have a heating function.

[0097] (1) Configure a 5wt.% GelMA solution that can be cured instantly as a photosensitive bioprinting ink, and add it to the liquid injection pump 8; the printing ink can maintain a stable strip shape while being irradiated by a high-energy ultraviolet light source 11 through the 3D printing nozzle 10 Hydrogel shape, and there is sufficient adhesion effect between each hydrogel. Turn on the submersible pump 13 and the constant temperature wate...

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PUM

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Abstract

The invention discloses a macroporous hydrogel 3D printing device which comprises a gas injection pump, a gas pressure meter, a T-shaped channel, a gas inlet capillary glass tube, an outlet capillary glass tube, a horizontal moving support, a water bath sleeve, a liquid injection pump, a flow meter, a D printing nozzle, a printing bottom plate, a submersible pump and a constant-temperature water bath heating device. The macroporous hydrogel prepared by the device disclosed by the invention not only can ensure good shape and dimensional accuracy on the appearance structure, but also can realize accurate control on the space position and the size of foam pores, and is extremely high in editability; the device provided by the invention can realize various types of macroporous hydrogel structures, including a uniform foam structure, a foam structure presenting density gradient and a foam structure presenting size gradient, by adjusting the gas phase flow and the like, and can generate multiple purposes.

Description

technical field [0001] The present invention relates to the field of microfluidics and the fields of medicine, biology, tissue engineering, etc., and in particular to a macroporous hydrogel 3D printing device and a printing method. Background technique [0002] Macroporous hydrogel materials refer to composite porous materials formed by further introducing micron to submillimeter macropores in addition to the porous structure of hydrogel polymers. Since the pore size of the hydrogel material itself is generally on the order of nanometers to several micrometers, these additional micrometer to submillimeter pores are generally called macropores in the art. Macroporous hydrogels have controllable porosity at both nanoscale and microscale, which not only provides swelling and interfacial properties of hydrogels, but also provides transport properties of macroporous materials. have been extensively researched and applied. For example, macroporous hydrogels can enhance cell surv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C64/106B29C64/379B33Y10/00B33Y50/02B33Y40/20B29K105/04
CPCB29C64/106B29C64/379B33Y10/00B33Y50/02B33Y40/20B29K2105/041
Inventor 许忠斌代齐民黄兴刘聪崔赟
Owner ZHEJIANG UNIV