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Rapid propagation method of angelica keiskei

A Ashitaba and fast technology, applied in the field of plant tissue culture, can solve the problems of limited timeliness of explant material collection, long induction time, etc., and achieve the effects of easy industrial operation, simple medium components, and good healing rate

Pending Publication Date: 2022-03-18
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

However, this method takes a long time to induce, and the timeliness of explants is limited.

Method used

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  • Rapid propagation method of angelica keiskei

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with examples.

[0024] The rapid propagation method of Ashitaba provided in the present embodiment comprises the following steps: (1) Seed disinfection: soak the Ashitaba seeds in water for 12 hours, peel off the outer testa of the seeds, wash them with running water, and place them on an ultra-clean workbench First, soak in 75% ethanol for 30s, rinse with sterile water for 2-3 times, and then use 0.1% HgCl 2 Soak for 12 minutes, and finally rinse with sterile water for 5 to 6 times;

[0025] (2) Cultivation of sterile seedlings: Inoculate the sterilized Ashitaba seeds on the MS basic medium and cultivate them in a light incubator for 15-20 days, wherein the culture temperature is 24-26° C., and the light intensity is 3000-5000 lx. The light time is 16h / d, and sterile seedlings are obtained. In this embodiment, the seedling growth medium is MS basic medium, and the pH is 5.7-5.8.

[0026] ...

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Abstract

The invention provides a rapid propagation method of angelica keiskei, which comprises the following steps: soaking, peeling and disinfecting angelica keiskei seeds, culturing in a seedling culture medium to generate aseptic seedlings, taking leaves on the aseptic seedlings as explants to carry out callus induction culture, and carrying out subculture, rooting induction and domestication transplanting to obtain the angelica keiskei. The rapid propagation of the angelica keiskei is realized. The method has the advantages that materials are convenient to obtain, timeliness is avoided, the culture period is short, the callus rate of calluses is high, regenerated plants are easy to root, the seedling rate is high, and the propagation speed and survival rate of angelica keiskei are increased.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of Ashitaba. Background technique [0002] Ashitaba (scientific name: Angelica keiskei (Miq.) Koidz.) is a perennial herb of the Umbelliferae family Angelica, mainly distributed in Japan, Korea and China. Ashitaba is a very valuable medicinal and edible plant with a very broad market prospect. Although my country has a certain history of cultivating Ashitaba, the cultivation area of ​​Ashitaba is not large and has not been well received. development and utilization. One of the main reasons for this is the reproductive problems of Ashitaba. Ashitaba is a perennial herbaceous plant. It takes 2 to 4 years for seed-propagated plants to bear fruit, the seed germination rate is also low, the process of seedling formation is complicated, and the market price is relatively expensive, so it is not easy to plant on a large scale. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/00
CPCA01H4/008A01H4/002A01G22/00
Inventor 刘虹赛米热·艾山覃瑞
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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