Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Inhibition of adenoviruses with felociclovir

An adenovirus, a technology of uses, applied in the direction of antiviral agents, medical preparations containing active ingredients, peptide/protein ingredients, etc.

Pending Publication Date: 2022-03-18
MICROBION +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent and unmet medical need for safe and effective anti-adenoviral drugs for use in methods of treating and / or preventing adenoviral infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inhibition of adenoviruses with felociclovir
  • Inhibition of adenoviruses with felociclovir
  • Inhibition of adenoviruses with felociclovir

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1. Cytopathic Effect (CPE) Reduction Assay to Measure Felociclovir Inhibition of Adenovirus 5 (AdV5)

[0084] Preparation and culture of human foreskin fibroblast (HFF) cells

[0085] Human foreskin tissue was obtained from the University of Alabama tissue procurement facility at Birmingham and was approved by the Institutional Review Board. Tissues were stored at 4°C in cell culture medium consisting of Minimal Essential Medium (MEM) with Earle's salts supplemented with 10% fetal bovine serum (FBS; HyClone, Inc., Logan, UT), and standard Concentrations of L-glutamine, amphotericin B (Fungizone) and vancomycin. The tissue is then placed in a phosphate-buffered saline solution, minced and rinsed to remove red blood cells. The tissue fragments were then resuspended in trypsin-EDTA solution and incubated at 37°C to disperse the cells, which were then collected by centrifugation. The cell pellet was then resuspended in 4ml medium and placed at 25cm 2 tissue cu...

Embodiment 2

[0102] Example 2. Neutral Red Survival Assay to Measure Felociclovir Inhibition of Adenovirus 6 (AdV6)

[0103] Neutrophil cytotoxicity assays are used to detect cell viability or drug cytotoxicity. The principle of the assay is based on the detection of living cells via the uptake dye neutral red. Neutral red is a diamine azine (eurhodine) dye that can stain lysosomes in living cells. Viable cells can take up Neutral Red and incorporate the dye into their lysosomes via active transport, but nonviable cells cannot take up this chromophore. Thus, after washing, living cells can release the incorporated dye under acidified extraction conditions. The amount of dye released can be quantified and used to determine the total number of viable cells or the cytotoxicity of a drug. Thus, cytotoxicity is expressed as a concentration-dependent decrease in the uptake of neutral red following exposure to the investigated compounds.

[0104] The neutral red survival assay was performed i...

Embodiment 3

[0106] Example 3. Immunofluorescence assay of human A549 cells infected with AdV5 or AdV6 treated with felociclovir

[0107] Human A549 cells on glass coverslips in 6-well plates were infected or mock-infected with AdV5 or AdV6 at an MOI of 5 PFU / cell. After 1 hour, felociclovir was added to a final concentration of 0, 4, 10 or 40 [mu]M. Twenty-seven hours after infection, A549 cells were fixed in paraformaldehyde (3.7% in PBS) and permeabilized with methanol. Cells were stained for adenovirus DNA binding protein and adenovirus hexon. Early in AdV infection (before AdV DNA replication), DBP staining will be antinuclear and uniform. As the infection progresses, DBP associates with replication centers. Centers of replication are initially small "dots" (each from an incoming AdV genome). As DNA replication occurs, the replication centers will amplify and "multiply." As the infection progresses, the nuclei become enlarged and deformed. The AdV hexon, the most abundant compon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the development of therapeutic and prophylactic agents for the treatment and / or prophylaxis of adenovirus infection in humans and other mammals. Methods of treating or preventing an adenoviral infection in a mammal by administering an effective amount of felociclovir (FCV) are disclosed.

Description

technical field [0001] The present invention is in the field of therapeutic and prophylactic agents for the treatment of adenoviral infections and diseases. Specifically, the present invention relates to the use of filociclovir (FCV) in the treatment and / or prevention of adenovirus infection in mammals, especially in the treatment and / or prevention of adenovirus infection in humans. Background technique [0002] Adenoviruses (AdV) are ubiquitous DNA viruses most commonly associated with pediatric diseases of the respiratory tract, gastrointestinal tract, and conjunctiva. Other rare clinical manifestations of AdV infection include hepatitis, and genitourinary and neurological symptoms (Khanal et al., Biomedicines, 6(1) (2018)). Although most adenoviral infections are self-limited in immunocompetent hosts, dissemination of disease may occur in immunocompromised patients and other vulnerable populations (eg, schoolchildren and recruits) in closed or crowded settings form and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61P31/12A61K31/7052A61K31/195
CPCA61P31/12A61K31/522A61P31/20
Inventor T·L·鲍林M·N·普里查德
Owner MICROBION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com