Primer probe combination for methylation detection of cervical cancer related genes and application of primer probe combination

Abstract

The invention discloses a primer probe combination for methylation detection of cervical cancer related genes and application of the primer probe combination. The primer probe combination comprises a primer pair and a probe which are used for detecting methylation of the PAX1 gene, the FAM19A4 gene and the miR124-2 gene. The primer combination for detecting methylation of PAX1, FAM19A4 and miR124-2 genes is designed by adopting a unique strategy, the primer probe combination has high specificity, can be used for detecting by directly utilizing a plasma sample, has a better detection effect, is high in specificity and sensitivity, is beneficial to relieving psychological stress and extra inconvenience of a subject, and can be used for detecting the methylation of the PAX1, FAM19A4 and miR124-2 genes. Gene methylation changes in the occurrence and development process of cervical cancer are tracked and detected, and the problem that the occurrence and development process of cervical cancer is difficult to monitor is solved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a combination of primers and probes for detecting cervical cancer-related gene methylation and an application thereof. Background technique [0002] Cervical cancer is a common malignant tumor in gynecology. The high incidence age of carcinoma in situ is 30 to 35 years old, and the age of invasive cancer is 45 to 55 years old. According to the data released by the National Cancer Center in 2018, 90% of cervical cancer patients have developed into invasive cancer when they are diagnosed , The survival rate is greatly reduced, and the prognosis is also poor. Therefore, early screening of cervical cancer is an important way to effectively prevent cervical cancer, and the early screening program of cervical cancer also provides favorable support for reducing the incidence and mortality of cervical cancer. [0003] In the current cervical cancer screening process, primary screening of human...

AI Technical Summary

Problems solved by technology

Compared with conventional cervical cancer screening and cervical cancer diagnosis methods, methylation detection mainly has the following characteristics: (1) It can make up for the shortcomings of the current domestic routine cervical cancer screening methods such as the high rate of missed detection of TCT detection and the high false positive rate of HPV detection ; (2) It can detect whether there are precancerous lesions in cervical cells earlier than conventional TCT detection and colposcopy; (3) It also has a good early warning effect in the follow-up monitoring of cervical cancer, so the degree of gene methylation can be used as a test Whether the cervical cells are normal, and the dynamic index of the occurrence and development of cervical cancer, but the currently commonly used method for detecting the methylation of cervical cancer-related genes needs to extract the genomic DNA of the cervical tissue to be tested, such as CN107287294A discloses a cervical cancer-related gene methylation Primers, probes, kits and their applications for the detection of methylation levels, frequent tissue testing can easily cause additional trauma and increase the psychological pressure of the subjects

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