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Preparation method and preparation device of exosome

A preparation device and exosome technology, which is applied in the field of exosome preparation, can solve the problems of uneven mixing of raw materials and affecting the preparation efficiency of exosomes, and achieve the effect of improving the preparation efficiency

Pending Publication Date: 2022-03-29
南京国青血液净化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing preparation device, when the storage box is processing test tubes to add preparation raw materials, there is a situation where too much preparation raw materials are injected at one time, resulting in uneven mixing of preparation raw materials, which affects the preparation efficiency of exosomes

Method used

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  • Preparation method and preparation device of exosome
  • Preparation method and preparation device of exosome
  • Preparation method and preparation device of exosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] see figure 1 As shown, the exosome preparation device described in this embodiment includes a box body 1 and a partition frame 11 arranged in the box body 1. The partition frame 11 separates the inside of the box body 1 to form a preparation chamber and a power chamber. The bottom of the chamber is provided with a turntable 13, and the power chamber is provided with a rotating mechanism 2. The rotating mechanism 2 is used to drive the turntable 13 to rotate. One side of the top surface of the turntable 13 is detachably inserted with a treatment test tube 3, and the top open end of the treatment test tube 3 is provided with There is a material storage box 4, the bottom opening of the material storage box 4 is located directly above the top opening of the processing test tube 3, the material storage box 4 transfers the raw materials stored in it to the processing test tube 3, and the raw materials for exosome preparation are rotated and separated in the processing test tub...

Embodiment 2

[0046] This embodiment adopts the same technical solution as Embodiment 1, the difference is, please refer to Figure 5 As shown, a suction mechanism 8 is provided at the bottom end of the rotary rod 5 , and the transmission assembly 7 selectively drives the rotary rod 5 to rotate through the suction mechanism 8 .

[0047] see Figure 6 As shown, the suction mechanism 8 includes a fixed sleeve 86. A sleeve 81 is arranged inside the fixed sleeve 86. A conductive coil 82 is wound on the outside of the sleeve 81. The bottom of the rotating rod 5 runs through the fixing sleeve 86 and the sleeve 81. The rotating rod 5 passes through the bearing Rotately connected with the fixed sleeve 86, the fixed sleeve 86 is fixed to the inner wall of the box body 1, the transmission assembly 7 also includes a support column 74, the support column 74 is fixedly passed through the driven pulley 73, and the top of the support column 74 is provided with a transmission cavity, the transmission cavit...

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Abstract

The invention provides an exosome preparation method and device, and relates to the technical field of exosome preparation.The preparation device comprises a box body and a partition frame arranged in the box body, the partition frame divides the interior of the box body into a preparation cavity and a power cavity, a rotating disc is arranged at the bottom in the preparation cavity, and a rotating mechanism is arranged in the power cavity; the rotating mechanism is used for driving the rotating disc to rotate, a treatment test tube is detachably inserted into one side of the top face of the rotating disc, a storage box is arranged at the top opening end of the treatment test tube, a bottom opening of the storage box is located over a top opening of the treatment test tube, and the storage box transfers raw materials stored in the storage box into the treatment test tube. The top opening rotation axis of the treatment test tube coincides with the rotation axis of the turntable; the intermittent feeding mechanism is arranged in the discharging pipe, so that preparation raw materials in the storage box are intermittently injected into the treatment test tube, the mixing efficiency of the preparation raw materials in the treatment test tube is guaranteed, and the preparation efficiency of the exosome is improved.

Description

technical field [0001] The invention relates to the technical field of exosome preparation, in particular to a preparation method and a preparation device of exosome. Background technique [0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins. Exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid, and breast milk. Exosomes are currently regarded as specifically secreted membrane vesicles that participate in intercellular communication. The current standard for exosome extraction is still to obtain exosomes by ultracentrifugation in the preparation device. [0003] In the existing preparation device, when the storage box is processing test tubes to add preparation materials, too many preparation materials are injected at one time, resulting in uneven mixing of preparation materials, which affects the preparation efficiency of exosomes. Contents of the invention [0004] Aiming at the deficiencies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/077
CPCC12M33/10C12N5/0653
Inventor 邓彦玮
Owner 南京国青血液净化科技有限公司
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