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Recombinant collagen hydrogel for injection and preparation method thereof

A technology for recombining collagen and recombining collagen, applied in the field of biomedical materials, can solve problems such as inability to improve skin damage, achieve the effects of non-immunogenicity and low risk of adverse reactions, improved relaxation, and good biocompatibility

Active Publication Date: 2022-04-01
JIANGSU JLAND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned hydrogels are only suitable for reducing facial sagging caused by natural aging, replenishing capacity loss and reshaping facial contours, etc., and cannot improve skin damage caused by photoaging

Method used

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  • Recombinant collagen hydrogel for injection and preparation method thereof
  • Recombinant collagen hydrogel for injection and preparation method thereof
  • Recombinant collagen hydrogel for injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1 g of recombinant collagen and 0.5 g of tea polyphenols were dissolved in 10 g of sodium hydroxide solution with a concentration of 1.25 mmol / L and mixed uniformly to obtain a premix solution with a pH value of 12. Then add 0.2g of 1,4-butanediol diglycidyl ether into the premix, mix well and place in a constant temperature water bath, set the reaction temperature to 60°C, and the reaction time to 1h to obtain the cross-linked recombinant collagen gel. glue. Then cut the cross-linked recombinant collagen gel into pieces with a volume of about 1cm 3 , washed with physiological saline for 24 hours, homogenized, potted, and sterilized to obtain a cross-linked recombinant collagen gel for injection.

Embodiment 2

[0035] 1 g of recombinant collagen and 1 g of tea polyphenols were dissolved in 10 g of sodium hydroxide solution with a concentration of 0.04 mmol / L and mixed uniformly to obtain a premix solution with a pH value of 10. Then add 0.2g of 1,4-butanediol diglycidyl ether into the premix, mix well and place in a constant temperature water bath, set the reaction temperature to 60°C, and the reaction time to 1h to obtain the cross-linked recombinant collagen gel. glue. Then cut the cross-linked recombinant collagen gel into pieces with a volume of about 1cm 3 , washed with physiological saline for 24 hours, homogenized, potted, and sterilized to obtain a cross-linked recombinant collagen gel for injection.

Embodiment 3

[0037] 1 g of recombinant collagen and 1 g of tea polyphenols were dissolved in 10 g of sodium hydroxide solution with a concentration of 1.25 mmol / L and mixed uniformly to obtain a premix solution with a pH value of 12. Then add 0.3g of 1,4-butanediol diglycidyl ether into the premixed solution, mix well and put it in a constant temperature water bath, set the reaction temperature to 60°C, and the reaction time to 1h to obtain the cross-linked recombinant collagen gel. glue. Then cut the cross-linked recombinant collagen gel into pieces with a volume of about 1cm 3 , washed with physiological saline for 24 hours, homogenized, potted, and sterilized to obtain a cross-linked recombinant collagen gel for injection.

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Abstract

The invention discloses recombinant collagen hydrogel for injection and a preparation method of the recombinant collagen hydrogel. The method comprises the following steps: dissolving recombinant collagen and tea polyphenol in an alkaline solution to obtain a premixed solution with the pH value of 9-13, adding a cross-linking agent, heating the mixed solution in a water bath, carrying out a cross-linking reaction to obtain a cross-linked recombinant collagen hydrogel, cutting the cross-linked recombinant collagen hydrogel into blocks, cleaning, homogenizing, filling, and sterilizing to obtain the recombinant collagen hydrogel. The recombinant collagen hydrogel for injection is obtained. The recombinant collagen hydrogel provided by the invention has excellent mechanical properties, can be used by injection, and improves skin injury caused by photoaging.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a recombinant collagen hydrogel for injection and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body and the first protective barrier of the body. It plays a vital role in resisting external aggression and maintaining the stability of the internal environment. Skin aging is a part of body aging, and body aging is most obvious on the skin. At present, skin aging is generally divided into inherent aging caused by genetic factors and force majeure factors (such as gravity, changes in body endocrine and immune function), also known as natural aging, and environmental factors such as solar radiation, exposure to chemicals, Skin aging caused by dryness is exogenous aging. Among them, photoaging specifically refers to skin aging caused by long-term ultraviolet exposure. The clinical symptoms of photoaging include wrin...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/50A61L27/52A61L27/54
Inventor 李梦杰李晨昱李冲王永会赵健烽
Owner JIANGSU JLAND BIOTECH CO LTD
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