Full-automatic cell separation device based on magnetic regulation and control

Abstract

A full-automatic cell separation device based on magnetic regulation and control comprises a screening chip which is located in a magnetic field and is used for separating target cells, and is characterized in that a soft magnet isolated from a blood sample is arranged in the chip.

Description

technical field [0001] The invention relates to the field of cell screening, in particular to a magnetic regulation automatic cell separation device. Background technique [0002] At present, there are three methods for enriching and capturing circulating tumor cells in peripheral blood: Method 1: 1. Lying and removing red blood cells; 2. Filtering out other cells such as white blood cells and platelets through microfluidic technology chips; 3. Using EpCAM immunomagnetic beads Positive enrichment captures circulating tumor cells; Method 2: 1. Cleavage and removal of red blood cells; 2. Removal of white blood cells by CD45 and CD15 immunomagnetic beads; 3. Negative enrichment of circulating tumor cells; Method 3: Based on the use of tumor cells and normal cells in different sizes ISET to isolate CTCs. The above method has the following defects: 1, lysing red blood cells, the erythrocyte lysate cannot lyse red blood cells 100% accurately, and will always affect other types of...

AI Technical Summary

Problems solved by technology

The above method has the following defects: 1, lysing red blood cells, the red blood cell lysate cannot lyse red blood cells 100% accurately, and will always affect other types of cells more or less

2. The blood sample processing process is complicated, time-consuming and requires professional operation

3. Negative enrichment is to remove leukocytes through leukocyte-associated antigen CD45, etc., and remove the leukocytes that are most likely to interfere with CTC detection to the greatest extent, so as to gradually remove plasma, red blood cells, and white blood cells in the blood, and retain the rare ones to the greatest extent. CTC, but often not of high purity

Under such a high pressure, the pore diameter of the membrane is deformed, and some tumor cells are filtered out through the deformed pores, resulting in low capture efficiency of tumor cells; at the same time, the change of cell structure will lead to the death of some tumor cells

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