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Method for determining content of total lignans in ginkgo leaf medicinal material, ginkgo leaf extract and single preparation of ginkgo leaf medicinal material

A technology for total lignans and extracts is applied in the field of total lignan content determination in ginkgo biloba medicinal materials, extracts and single-flavor preparations, and can solve the problem that the total lignan content and wood content of ginkgo biloba medicinal materials cannot be accurately determined. There are problems such as interference and inability to remove lipoids in the determination of the content of lipoproteins, which achieves comprehensive quality control and quality standard improvement, ensures stability and uniformity, and is easy to operate.

Pending Publication Date: 2022-04-01
山西振东泰盛制药有限公司
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  • Description
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AI Technical Summary

Problems solved by technology

[0005] However, the C18 or polyamide solid-phase extraction used in the pretreatment of these samples cannot remove flavonoids and other components in Ginkgo biloba and its preparations well, which interferes with the determination of lignan components, so that it is impossible to accurately determine Ginkgo biloba. The content of total lignans in substances and their preparations is not conducive to their effective quality control

Method used

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  • Method for determining content of total lignans in ginkgo leaf medicinal material, ginkgo leaf extract and single preparation of ginkgo leaf medicinal material
  • Method for determining content of total lignans in ginkgo leaf medicinal material, ginkgo leaf extract and single preparation of ginkgo leaf medicinal material
  • Method for determining content of total lignans in ginkgo leaf medicinal material, ginkgo leaf extract and single preparation of ginkgo leaf medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 assay method

[0032] (1) Instruments and reagents

[0033] Shimadzu UV-2600 ultraviolet spectrophotometer (Shimadzu, Japan); Mettler Toledo MS105 electronic analytical balance (Mettler Toledo, Switzerland); Milli-Q Advantage A10 pure water machine (Millipore, USA); KQ-250DE ultrasonic cleaner (Kunshan City Ultrasonic Instrument Co., Ltd., China).

[0034] Methanol (chromatographically pure, Admas, China); dihydrodehydrodiguaiacol 4-O-β-D-glucoside (Urolignoside) reference substance (prepared by the laboratory, purity 98.0%); Cleanert Alumina N A permanent alumina extraction column (1g / 6ml, 100-200 mesh).

[0035] Shuxuening injection was provided by Shanxi Zhendong Taisheng Pharmaceutical Co., Ltd., batch number: 1905041.

[0036] Shuxuening injection was provided by Shanxi Zhendong Taisheng Pharmaceutical Co., Ltd., batch number: 1905261.

[0037] (2) Preparation of reference substance solution

[0038] Accurately weigh 10.41mg of dihydrodehydrodigua...

Embodiment 2

[0044] Compared with Example 1, the batch number of Ginkgo biloba extract is 1905061, and other conditions are not changed, and the total lignan content is 14.9%.

Embodiment 3

[0046] Compared with Example 1, the batch number of Ginkgo biloba extract is 1905041, other conditions are not changed, and the total lignan content is 14.3%.

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Abstract

The invention provides a method for determining the content of total lignans in a ginkgo leaf medicinal material, an extract and a single preparation of the ginkgo leaf medicinal material, and belongs to the technical field of traditional Chinese medicine detection. Comprising the following steps: S1, preparing a reference solution; s2, preparing a test solution; s3, detecting, namely drawing a standard curve by taking the concentration of dihydrodehydrodiguaiacol 4-O-beta-D-glucoside as a horizontal coordinate and taking the absorbance difference value delta A measured at UV280nm and 335nm as a vertical coordinate, wherein the concentration of the dihydrodehydrodiguaiacol 4-O-beta-D-glucoside is taken as a horizontal coordinate and the absorbance difference value delta A measured at UV280nm and 335nm is taken as a vertical coordinate; and taking the test sample solution to measure delta A, calculating the concentration of Urolignoside in the test sample solution according to the standard curve, and calculating the content of total lignans in the sample. According to the method, the quality control components of the ginkgo leaf medicinal material, the ginkgo leaf extract and the single preparation thereof are perfected, so that the comprehensive quality control and quality standard improvement of the ginkgo leaf medicinal material, the ginkgo leaf extract and the single preparation thereof can be better carried out, and the stability and uniformity of the product are ensured.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the content of total lignans in ginkgo biloba medicinal materials, extracts and single herb preparations thereof. Background technique [0002] Ginkgo biloba is the most commonly used medicinal plant, and Ginkgo biloba preparations are mainly used to treat cardiovascular and cerebrovascular diseases. The chemical components in Ginkgo biloba and its preparations are mainly flavonoids, ginkgolides and lignans, of which flavonoids and ginkgolides have been studied more, and the latest research has found that lignans have an effect on cerebral ischemic injury, Hyperlipidemia, myocardial ischemia-reperfusion injury, etc. have a good therapeutic effect. In the existing quality research and quality standards of ginkgo biloba medicinal materials, extracts and their preparations, the quality control indicators are mainly flavonol gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/28
Inventor 高治华韩佩莲林珊孙欣光周丽娟杨晓宁朱平
Owner 山西振东泰盛制药有限公司
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