Microsampling method for organ morphogenesis period of Chinese narcissus

A technology of morphogenesis and daffodils, which is applied in the field of plant tissue sampling, can solve the problems of high skill requirements and difficult practical application of laser microdissection technology, and achieve the effect of simple operation and guaranteed integrity

Pending Publication Date: 2022-04-05
INT CENT FOR BAMBOO & RATTAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the laser microdissection technology requires too high operational skills of the experimenters, so

Method used

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  • Microsampling method for organ morphogenesis period of Chinese narcissus
  • Microsampling method for organ morphogenesis period of Chinese narcissus
  • Microsampling method for organ morphogenesis period of Chinese narcissus

Examples

Experimental program
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Effect test

Embodiment 1

[0049] This embodiment provides a microscopic sampling method of different tissue parts during the morphological construction period of Chinese narcissus 'Calendula Yintai'. In this method, when the stamens are in the original base shape, the stamens are spherical or cylindrical, and they are in the same shape as the receptacle. If the combination is not tight, the method specifically includes: dissecting and stripping the bracts, perianth, stamens, pistil-receptacle assembly under a stereomicroscope, and quick-freezing the stripped tissues in liquid nitrogen. The sampling steps are as follows:

[0050] (1) Peeling off the flower buds: Cut across the top at 1 / 2 of the height of the bulb bulb, cut 1 lengthwise at least 1 cm from the four sides of the main flower bud, lay one side flat and cut across the bulb disc, and finally cut into Rectangular block; before sampling, the residual scales and leaves were peeled off one by one until the flat flower buds were completely exposed. ...

Embodiment 2

[0058] This embodiment provides a microscopic sampling method for different tissue parts of the Chinese narcissus 'Calendula Yintai' during the morphological establishment of the flower organs. It is long cylindrical, with 3 longitudinal grooves formed on the inner side, and the outer base is more firmly connected to the receptacle. The difference between this method and the method in Example 1 lies in the following steps:

[0059] (3) Peel off the first round of perianth: under a stereomicroscope, place one tip of the dissecting tweezers horizontally on the junction of the top of the first round of perianth, and press down slightly along the junction of the perianth to leave the top of the perianth. Then use dissecting tweezers to clamp the inner and outer sides of the perianth and tear it obliquely downward, and then quickly pick the first round of perianth into a centrifuge tube floating on the surface of liquid nitrogen for quick freezing.

[0060] (4) Peel off the second ...

Embodiment 3

[0065] This embodiment provides a microscopic sampling method for different tissue parts of the Chinese narcissus 'Yu Linglong' during the morphological establishment of the flower organs. In this method, when the petalized stamens are in the original base shape, the petalized stamens are spherical or cochlea-shaped, and The receptacle is not tightly combined, and the fragmented corona has not yet developed. The method specifically includes: microscopically dissecting and extracting a small amount of bracts, perianth, petalized stamens, and pistil-receptacle assembly, followed by quick-frozen storage in liquid nitrogen; the method and examples The difference of method 1 is only that: in step (5), the sampled tissue is the primordia of petalized stamens.

[0066] This method is used to separate the tissue parts of the largest florets in the inflorescence of the Chinese narcissus 'Yulinglong' three-year-old commercial bulb bulb in the developmental morphological construction stag...

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Abstract

The invention relates to the technical field of plant tissue sampling methods, in particular to a microscopic sampling method for a Chinese narcissus organ morphogenesis period. The microsampling method provided by the invention comprises the following steps: firstly stripping buds, stripping bract pieces, then stripping a first round of flower quilt, a second round of flower quilt, stamens or petaloid stamens in sequence, and finally stripping an auxiliary crown-pistil-receptacle combination. Wherein before the auxiliary crown is formed, the method for stripping the first round of flower quilt comprises the following steps: under a stereoscopic microscope, transversely placing the tip of a dissecting needle at the joint of the top of the first round of flower quilt, pressing the top of the flower quilt downwards and forcibly along the joint of the flower quilt, and inserting the dissecting needle into the inner side of the flower quilt to exert force outwards to obtain the stripped first round of flower quilt. The method not only can well ensure the integrity of different floral organs and the accuracy of stripping, but also is simple to operate, does not need complex instruments, equipment and operation steps, and is suitable for popularization and application in researches such as development of the Chinese narcissus and morphological establishment of the floral organs.

Description

technical field [0001] The invention relates to the technical field of plant tissue sampling methods, in particular to a microscopic sampling method for Chinese daffodils in the stage of organ morphogenesis. Background technique [0002] Flower is the main reproductive organ of higher plants and an important basis for the study of plant evolution and classification. Floral development in plants is a complex multi-step process in which a series of endogenous and exogenous factors lead to the transformation of meristem cells and the initiation of floral development. The differentiation process of meristem cells is as follows: the shoot apical meristem first transforms into the inflorescence meristem, then produces the floral meristem, and finally differentiates into each whorl of floral organs, and finally completes the morphological establishment of each floral organ. The flower structure of dicotyledons is generally four concentric circles. The typical flower structure cons...

Claims

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Application Information

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IPC IPC(8): G01N1/08G01N1/42
Inventor 胡陶马艳军江泽慧王雁胡晓萌范可可邓雅云
Owner INT CENT FOR BAMBOO & RATTAN
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