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Disease-resistant regulation function application of rape gene BnCAMTA3

A functional application and technology of rapeseed, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of limited range of available disease-resistant resources and long breeding cycle, etc., to achieve a wide range of available disease-resistant resources, short breeding cycle, Fast results in breeding

Active Publication Date: 2022-04-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to natural genetic isolation, traditional disease-resistant breeding has significantly limited the available range of disease-resistant resources. Only disease-resistant resources with close genetic relationships can be used, and multiple crosses and backcrosses are required. Therefore, the breeding cycle is long and requires A lot of manpower and material resources

Method used

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  • Disease-resistant regulation function application of rape gene BnCAMTA3
  • Disease-resistant regulation function application of rape gene BnCAMTA3

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Experimental program
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Effect test

Embodiment 1

[0037] The present invention clones a rapeseed gene BnCAMTA3, clarifies the regulatory function of the gene against Sclerotinia sclerotiorum by constructing overexpression transgenic rapeseed for the first time, and reveals that the gene has an important positive regulation function on Sclerotinia sclerotiorum resistance. Because the overexpression of the BnCAMTA3 gene leads to a significant increase in the resistance of rapeseed to Sclerotinia sclerotiorum, it is possible to create and obtain new anti-sclerotinia rapeseed materials by constructing transgenic rapeseed with overexpression of this gene and using genetic engineering technology. The main steps include:

[0038] 1) Cloning and preservation of rapeseed BnCAMTA3 gene

[0039] The rapeseed BnCAMTA3 gene provided by the invention is cloned through the following steps. Primers BnCAMTA3-F (5'-atg gcg gaa gca aga cga ttt-3') (sequence shown in SEQ ID: 3), and BnCAMTA3-R (5'-agc gtt cca caa agt tga gga-3') (sequence show...

Embodiment 2

[0067] Example 1 of the present invention clarifies the positive regulation function of rapeseed gene BnCAMTA3 on Sclerotinia sclerotiorum resistance by constructing overexpression plants of BnCAMTA3 gene. This example describes the RNAi rapeseed construction technology of the BnCAMTA3 gene, and further proves the positive regulatory function of BnCAMTA3 on Sclerotinia sclerotiorum resistance through this material. This example provides a technique for creating new rapeseed materials with reduced resistance to Sclerotinia sclerotiorum by constructing RNAi rapeseed of BnCAMTA3 gene, and these materials can be used for analysis of gene function and mechanism of action. The main steps include:

[0068] (1) Construction and acquisition of BnCAMTA3 gene RNAi structure

[0069] Primer BnCAMTA3-F3 (5'-ggagaggacacgctcgagatg gcg gaa gca aga cgt-3' was designed according to the BnCAMTA3 sequence cloned in the present invention, and the italic part is a sequence that includes the Xho I ...

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Abstract

The invention provides an application of a disease-resistant regulation function of a rape gene BnCAMTA3, and particularly provides an application of the rape gene BnCAMTA3 in prevention and control of sclerotiniose, which is an application in obtaining a rape material with changed disease resistance by creating transgenic rape (Brassica napus). According to the invention, overexpression of BnCAMTA3 and RNAi transgenic oilseed rape are constructed, a positive regulation function of the gene on sclerotiniose resistance is disclosed for the first time, and application of obtaining an oilseed rape material with reduced sclerotiniose resistance by creating BnCAMTA3-RNAi oilseed rape and application of obtaining an anti-sclerotiniose oilseed rape material by creating BnCAMTA3 overexpression oilseed rape are provided. The BnCAMTA3 gene provided by the invention is a new gene resource suitable for creating and breeding new sclerotiniose-resistant rape materials and new varieties, and is of great significance to green prevention and control of sclerotiniose.

Description

technical field [0001] The invention belongs to the field of plant disease resistance biology technology, and relates to the application of a rapeseed gene BnCAMTA3 for regulating disease resistance, in particular to the application of the rapeseed gene BnCAMTA3 in the prevention and control of sclerotinia. Background technique [0002] 1. Plant gene function analysis technology [0003] The function of plant genes is carried out through gene expression and protein accumulation. Therefore, gene function is usually judged and clarified by comparing and analyzing the phenotype (Phenotype) or function of the gene under normal expression and abnormal expression. Overexpression of genes includes two categories: higher than normal expression and lower than normal expression. The expression above the normal level is mainly overexpression / overexpression (Over-expression), which is mainly achieved by connecting a strong promoter to drive the expression of the target gene. The expr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84A01H5/00A01H6/20
Inventor 蔡新忠任春秀杨玉涵
Owner ZHEJIANG UNIV