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Stropharia rugoso-annulata liquid reducing strain preparation and sporocarp cultivation method

A technology of stropharia stropharia and liquid strains, applied in mushroom cultivation, botanical equipment and methods, cultivation, etc., can solve the problems of low production efficiency and long preparation cycle, reduce production and transportation costs, and facilitate expanded cultivation , the effect of increasing biomass

Pending Publication Date: 2022-04-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] In view of this, the purpose of the present invention is to provide a method for preparing the liquid-reduced strain of Stropharia stropharia and a method for cultivating fruiting bodies using the reduced strain, so as to solve the problem of the long preparation cycle and low production efficiency of the existing Stropharia stropharia strains. technical issues

Method used

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  • Stropharia rugoso-annulata liquid reducing strain preparation and sporocarp cultivation method
  • Stropharia rugoso-annulata liquid reducing strain preparation and sporocarp cultivation method

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preparation example Construction

[0024] Therefore, in the first aspect of the present invention, a kind of preparation method of Stropharia stropharia liquid-reduced bacterial classification is provided, and the method comprises the following steps:

[0025] (1) Activation of Stropharia stropharia strains: Inoculate the mycelium of Stropharia stropharia into PDA medium, culture at 20-28°C for 10-15 days, and obtain the mother species;

[0026] (2) Preparation of first-level liquid strains: transfer the mother species to the first-level liquid strain culture medium, culture at 150-180rpm, 20-28°C in a light-proof shaking table for 7-10d, and obtain the first-level liquid strains ;

[0027] (3) Preparation of secondary liquid strains: inoculating the primary liquid strains into the secondary liquid strain culture medium, and fermenting at 20-28°C for 3-5 days to obtain secondary liquid strains;

[0028] (4) Preparation of liquid reduced strains: mixing the mycelial balls of the secondary liquid strains with th...

Embodiment 1

[0062] Embodiment 1, the method for the fruiting body cultivation of Stropharia grandis (CGMCC No.52220)

[0063] 1. Cultivation process

[0064] The cultivation process of the fruiting bodies of Stropharia stropharcota (CGMCC No.52220) is as follows: mother species activation→first-level liquid strain preparation→second-level liquid strain preparation→inoculation→cultivation seed germination management→sowing and covering soil→fruiting Management → fruiting body harvesting.

[0065] (1) Activation of the mother species: inoculate the original strain of Stropharia grandis (CGMCC No.52220) stored at -80°C or 4°C on the mother seed medium (PDA test tube slant / plate), and keep away from light at 25°C Cultivate, cultivate until the mycelia are covered with medium, and the obtained mycelium is used as the mother species ( figure 1 ).

[0066] Mother PDA medium: Take 200g of peeled potato slices, put them in 900mL of water and boil for 20-30min, take the filtrate and add 20g of a...

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Abstract

The invention belongs to the technical field of edible fungus cultivation, and relates to a preparation method of a S. rugosoannulata liquid reduction strain, which comprises the following steps: (1) activation of a S. rugosoannulata strain: inoculating S. rugosoannulata mycelium to a PDA culture medium, and culturing at 20-28 DEG C for 10-15 days to obtain a mother strain; (2) preparing a first-stage liquid strain: transferring the mother strain into a first-stage liquid strain culture medium, and culturing in a shaker in a dark place at the speed of 150-180 rpm and the temperature of 20-28 DEG C for 7-10 days to obtain the first-stage liquid strain; (3) preparing a secondary liquid strain: inoculating the primary liquid strain into a secondary liquid strain culture medium, and fermenting at 20-28 DEG C for 3-5 days to obtain the secondary liquid strain; and (4) preparing a liquid reducing strain: mixing the mycelium pellets of the secondary liquid strain with a reducing strain culture solution to obtain the liquid reducing strain. The invention also relates to a method for cultivating stropharia rugoso-annulata sporocarp by using the liquid reduction strain. According to the method, the culture period of the stropharia rugoso-annulata strain can be greatly shortened.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and relates to a method for preparing liquid-reduced strains of Stropharia stropharii and cultivating its fruiting bodies. Background technique [0002] Stropharia rugosoannulata Farl.ex Murrill, also known as stropharia rugosoannulata Farl.ex Murrill, is also known as stropharia rugosoannulata Farl.ex Murrill, commonly known as red matsutake and red matsutake. ), Stropharia, which has a crisp and tender taste, delicious taste, rich in various nutrients such as protein, taurine and flavonoids. As a characteristic edible fungus recommended by the Food and Agriculture Organization of the United Nations (FAO) to developing countries, it is widely traded in the international edible fungus market. Stropharia has a wide range of cultivation substrates, such as various agricultural and forestry wastes, straw, and other edible fungus residues, etc., can be used as cultivation substrate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
Inventor 杨莹董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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