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Method for determining contents of three flavonoid chemical components in folium apocyni veneti

A determination method and technology for chemical components are applied in the field of content determination of three flavonoid chemical components in apocynum leaves, which can solve the problems of not being retrieved and unable to fully reflect the quality of medicinal materials.

Active Publication Date: 2022-04-15
JILIN INST OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0017] At present, the 2020 edition of the Pharmacopoeia of the People's Republic of China only stipulates the determination of the content of hyperin in the leaves of Apocynum apocynum by HPLC, which obviously cannot fully reflect the quality of the medicinal materials
In literature reports, HPLC method is often used to determine the content of one or more components in Apocynum leaf medicinal materials or its preparations, but no ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) is used to simultaneously determine the content of apocynum leaves. Quantitative reporting of multiple components

Method used

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  • Method for determining contents of three flavonoid chemical components in folium apocyni veneti
  • Method for determining contents of three flavonoid chemical components in folium apocyni veneti
  • Method for determining contents of three flavonoid chemical components in folium apocyni veneti

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Experimental program
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Effect test

experiment example 1

[0034] Experimental example 1 content determination method investigation

[0035] 1 Instruments and reagents

[0036]ACQUITY UPLC H-CLASS (equipped with quaternary solvent manager, sample manager FTN, PDA detector, American Waters Company); XEVO TQ-S triple quadrupole mass spectrometer (American Waters Company); MS105 electronic analytical balance (Swiss Mettler Toleduo Company); XH-300UL-2 dual-frequency ultrasonic microwave ultraviolet combination instrument (Beijing Xianghu Technology Development Co., Ltd.); KQ-5200DE ultrasonic cleaning instrument (Kunshan Ultrasonic Instrument Co., Ltd.); H1850 desktop high-speed centrifuge (Changsha Xiangyi Centrifuge Instrument Co., Ltd.); 1000Y high-speed multifunctional pulverizer (Yongkang Boou Hardware Products Co., Ltd.); RE-3000A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); HH-4 water bath (Shanghai Lichen Bangxi Instrument Technology Co., Ltd.);

[0037] Chlorogenic acid (batch number MUST-13031401, purit...

Embodiment 1

[0066] A method for simultaneously determining the content of three flavonoid chemical components in the leaves of Apocynum apocynum:

[0067] The chromatographic conditions are: the chromatographic column is HSS C18 SB (2.1×100mm, 1.8μm); the mobile phase is acetonitrile (B) -0.2% formic acid water (D), and the gradient elution condition is 0-1 min, 5%-9 %B; 1-1.6 min, 9%-10%B; 1.6-2.0min, 10%-13%B; 2-5 min, 13%-14%B; 5-8 min, 14%B; 10 min, 14%-30%B; 10-11 min, 30%-45%B; 11-11.5 min, 45%-60%B; 11.5-12 min, 60%-90%B; 12-12.5 min , 90-5% B; volume flow rate 0.3mL•min-1; injection volume 1 μL; injection chamber temperature 20 °C; column temperature 38 °C.

[0068] Mass spectrometry conditions are: ESI ion source, simultaneous scanning and detection in positive and negative ion modes, scanning range (m / z): 100-1000, capillary voltage 2.8 kV, ion source temperature 500 ℃, desolvation gas N2, gas flow 700 L h -1, collision gas argon, collision gas flow 0.1 mL•min-1, MRM quantitativ...

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Abstract

The invention belongs to the field of quality control of traditional Chinese medicines, and particularly relates to a method for determining the content of three flavonoid chemical components in folium apocyni veneti by adopting an ultrahigh liquid phase-tandem mass spectrometry method. According to the ultra-high performance liquid chromatography-tandem mass spectrometry technology adopted by the invention, ultra-high performance liquid chromatography is utilized to separate chemical components, and qualitative and quantitative analysis of a to-be-detected substance is completed by mass spectrometry. The method has the advantages of high selectivity, large linear range, high sensitivity, high accuracy and precision, short analysis time, simple sample treatment steps and the like, and is suitable for quality analysis of foods and medicines with large content difference of isoquercitrin, astragalus smicus glycoside and acetyl isoquercitrin; a brand new detection method can be provided for quality evaluation research of food and medicinal materials containing isoquercitrin, astragalus smicus glycoside and acetyl isoquercitrin and pharmacopoeia improvement of folium apocyni veneti, and meanwhile, a technical reference can be provided for in-vivo analysis research of three compounds of isoquercitrin, astragalus smicus glycoside and acetyl isoquercitrin.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and in particular relates to a content determination method of three flavonoid chemical components in apocynum leaves. Background technique [0002] Apocynum venetum L. is the leaf of Apocynum venetum L., which is a traditional Chinese medicine in my country. It has the effects of calming the liver and calming the nerves, clearing heat and diuresis, and is mainly used to treat liver yang dizziness, palpitations, insomnia, edema and oliguria, etc. disease. Studies have shown that the main active ingredients of Apocynum apocynum are flavonoids, which have various pharmacological activities such as lowering blood pressure, anti-oxidation, anti-depression, anti-anxiety and lowering cholesterol. Apocynum leaves are mainly distributed in Xinjiang, Qinghai, Gansu, Shaanxi, Shanxi, Henan, Hebei, Jiangsu, Shandong, Liaoning and Inner Mongolia and other provinces and regions ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCY02A50/30
Inventor 陈帅王慧竹徐畅
Owner JILIN INST OF CHEM TECH
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