Gold nanoparticles and immunodetection test strip

A gold nanoparticle, immune detection technology, applied in the field of immune detection, can solve the problems of increasing the technical difficulty of the process and the cost of manpower and material resources.

Pending Publication Date: 2022-04-15
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although this type of technology achieves signal amplification, it also increases the difficulty of process technology and t

Method used

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  • Gold nanoparticles and immunodetection test strip
  • Gold nanoparticles and immunodetection test strip
  • Gold nanoparticles and immunodetection test strip

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Experimental program
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Embodiment 1

[0043] This embodiment provides a gold nanoparticle. The preparation method of the gold nanoparticle is as follows: mix ascorbic acid and chloroauric acid with a molar ratio of 5:1, react in a water bath at 80°C for 30 minutes, centrifuge, redissolve in deionized water, and obtain gold dispersion of nanoparticles. refer to figure 1 , The results of the electron microscope show that the particle size of the gold nanoparticles in this embodiment is 20-40nm, which is relatively uniform, and the morphology is generally spherical.

Embodiment 2

[0045] This embodiment provides a gold nanoparticle. The difference from Embodiment 1 is that the same molar amount of potassium gluconate is used instead of ascorbic acid, so that the molar ratio of potassium gluconate to chloroauric acid in the reaction raw materials is 5:1.

Embodiment 3

[0047] This embodiment provides a gold nanoparticle, which differs from the embodiment 1 in that sodium salicylate (sodium 2-hydroxybenzoate) and chloroauric acid are mixed and reacted at a molar ratio of 10:1.

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Abstract

The invention discloses gold nanoparticles and an immunodetection test strip. On the first aspect, the invention provides the preparation method of the gold nanoparticles, and the preparation method comprises the following steps: mixing chloroauric acid with weak ionized small organic molecules, and reacting at 70-100 DEG C to obtain the gold nanoparticles. In the experiment process, the applicant finds that when sodium citrate and the like are used as reducing agents, due to the strong ionization characteristic of the sodium citrate and the like, the finally prepared gold nanoparticles are in a strong ionization state, adsorption between the gold nanoparticles and antibody protein is not facilitated, the total amount of antibodies combined with the surfaces of the gold nanoparticles is low, and sensitivity is not high. Compared with the prior art, according to the scheme, weakly ionized reducing organic small molecules are adopted as a reducing agent of chloroauric acid in a breakthrough mode, the gold nanoparticles obtained through reaction under the condition of 70-100 DEG C have the weakly ionized property, the larger total adsorption amount of surface antibody protein can be provided, and therefore the detection sensitivity is effectively improved.

Description

technical field [0001] The application relates to the technical field of immunoassay, in particular to gold nanoparticles and immunoassay test strips. Background technique [0002] Most of the existing immunoassay strips use gold nanoparticles (or colloidal gold) as immune markers to label proteins to form gold-labeled antibodies. During detection, color development is observed based on the gold-labeled antibodies on the binding pad. Gold nanoparticles are generally reacted with reducing agents such as chloroauric acid and sodium citrate, but the formed gold-labeled antibody is difficult to achieve high-sensitivity immunodetection. In order to improve the sensitivity of gold nanoparticle immunoassay test strips, the current improvement method is mainly through cascade enzymatic reaction, on the basis of gold nanoparticle color development and incubation reaction with chromogenic or luminescent substrates for secondary signal amplification, For example, by comodifying horser...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/558
CPCG01N33/54313G01N33/558
Inventor 蒋兴宇张江江
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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