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Mushroom sporocarp model cultivation method

A cultivation method and fruiting body technology, applied in the field of mushroom fruiting body model cultivation, can solve the problems such as long cultivation period of mushroom fruiting body, and achieve the effects of shortening fruiting time, shortening maturity cycle, and reducing time cost

Active Publication Date: 2022-04-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the above defects or improvement needs of the prior art, the present invention provides a method for cultivating mushroom sporocarp models, the purpose of which is to cultivate the mushroom sporocarp models by inoculating and planting fresh corn stalk slices twice, and reducing the culture period from 70 to 110 The days are shortened to 25-35 days, which solves the technical problem of the long cultivation period of mushroom fruiting bodies under the existing experimental conditions

Method used

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  • Mushroom sporocarp model cultivation method

Examples

Experimental program
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Embodiment 1

[0051] Embodiment 1 adopts fresh corn stalk sheet to cultivate mushroom fruiting body

[0052] (1) One-time inoculation and planting of corn stalks: inoculate the inoculation block of shiitake mushroom mycelia after the activation of the solid medium onto the flat surface of fresh corn stalk slices, such as Figure 4 As shown in A, in an airtight, airtight and aseptic environment, control the humidity above 80%, and cultivate at room temperature in the dark for 5-10 days until the mushroom mycelium covers the surface of the fresh corn stalk slices, such as Figure 4 As shown in B; the specific steps are as follows:

[0053] (1-1) Activation of Lentinus edodes strain Wuxiang No. 1 (W1):

[0054] In the ultra-clean workbench, inoculate the hyphae of the shiitake mushroom strain W1 preserved at low temperature on the solid MYG medium, seal and culture at 25°C for 7 days, and wait until the solid MYG medium is covered with mycelia for later use.

[0055] Solid malt extract mediu...

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Abstract

The invention discloses a mushroom sporocarp model cultivation method which comprises the following steps: (1) primary inoculation and field planting of corn straws: inoculating mushroom mycelium inoculation blocks to the surfaces of tiled fresh corn straw slices, culturing in a breathable closed sterile environment, controlling the humidity of the culture environment to be greater than 80%, culturing at room temperature in a dark place, and performing primary inoculation and field planting of the corn straws; the mushroom mycelia cover the surfaces of the fresh corn straw slices; (2) secondary inoculation and field planting of the corn straw: removing the mushroom mycelium inoculation blocks in the step (1), vertically inserting the fresh corn straw slices, enabling the bottoms of the fresh corn straw slices to be in contact with the tiled fresh corn straw slices covered with the mushroom mycelia, controlling the humidity of the culture environment to be greater than 80% in a breathable closed sterile environment, and culturing at room temperature in a dark place, and when the vertically inserted fresh corn straw slices are overgrown with mycelia, continuing dark culture or light culture until the mushroom mycelia form sporocarp. By the adoption of the method, the mushroom culture period is shortened to 25-35 days.

Description

technical field [0001] The invention belongs to the field of edible fungus breeding, and more specifically relates to a method for cultivating mushroom fruiting body models. Background technique [0002] Lentinula edodes belongs to the fungi kingdom Basidiomycota, Agaricomycetes, Agaricales, Lentinula. Lentinus edodes is rich in nutrition, delicious in taste, unique in fragrance, rich in essential amino acids and dietary fiber, among which lentinan has anti-tumor activity, and is one of the important edible fungi in the world. The artificial cultivation of shiitake mushrooms is mainly concentrated in China, Japan, and South Korea, among which China contributes more than 80% of the world's shiitake mushroom production. my country is the first country in the world to start artificial cultivation. It has a history of more than 800 years. From segment wood cultivation to substitute material cultivation, to today's highly automated factory cultivation, its cultivation methods ar...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/40A01G18/20
Inventor 康恒牟春叶龚钰华陈新边银丙
Owner HUAZHONG AGRI UNIV
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