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Cryopreservation liquid and cryopreservation method for bone tissues and bone soft tissues

A cryopreservation method and cryopreservation liquid technology, which is applied in the field of cryopreservation liquid for bone tissue and soft tissue, can solve the problems of low cell survival rate and excessive toxicity, and achieve the goal of expanding clinical application, reducing damage and prolonging storage time Effect

Pending Publication Date: 2022-04-22
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the cryopreservation of bone tissue and bone soft tissue, the existing cryopreservation solutions and cryopreservation methods have the problems of strong toxicity and low cell survival rate, so it is necessary to develop new cryopreservation solutions and methods for bone tissue and bone soft tissue. Freezing method

Method used

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  • Cryopreservation liquid and cryopreservation method for bone tissues and bone soft tissues
  • Cryopreservation liquid and cryopreservation method for bone tissues and bone soft tissues
  • Cryopreservation liquid and cryopreservation method for bone tissues and bone soft tissues

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Embodiment 1

[0048] Bone tissue and bone soft tissue cryopreservation liquid of the present invention, every 1000ml contains KOH 80-120mmol, HCl 40mmol, NaHCO 3 10mmol, NaH 2 PO 4 0.8mmol, MgSO 4 0.8mmol, CaCl 2 0.8 mmol, 2 mmol of glucose, 80 mmol of TES, 80 ml of 1,2 propylene glycol, 80 ml of 99.9% dimethyl sulfoxide, and the balance is deionized water. The pH value of the bone tissue and soft tissue cryopreservation solution is 7.3-7.5. Aliquot, seal, and store at 0-4°C.

Embodiment 2

[0050] Bone tissue and soft tissue freezing solution of the present invention, bone tissue and soft tissue freezing solution of the present invention, every 1000ml contains KOH 120mmol, HCl 80mmol, NaHCO 3 50mmol, NaH 2 PO 4 1.2mmol, MgSO 4 1.2mmol, CaCl 2 1.2 mmol, 8 mmol of glucose, 120 mmol of TES, 120 ml of 1,2 propylene glycol, 120 ml of 99.9% dimethyl sulfoxide, and the balance is deionized water. The pH value of the bone tissue and soft tissue cryopreservation solution is 7.3-7.5. Aliquot, seal, and store at 0-4°C.

Embodiment 3

[0052] Bone tissue and bone soft tissue cryopreservation liquid of the present invention, every 1000ml contains KOH 100mmol, HCl 60mmol, NaHCO 3 30mmol, NaH 2 PO 4 1mmol, MgSO 4 1mmol, CaCl 2 1 mmol, 5 mmol of glucose, 100 mmol of 2-[[tris(hydroxymethyl)methyl]amino]ethanesulfonic acid, 100 ml of 1,2 propylene glycol, 100 ml of 99.9% dimethyl sulfoxide, and the balance was deionized water. The pH value of tissue and soft tissue cryopreservation solution is 7.3-7.5. Aliquot, seal, and store at 0-4°C.

[0053] The method for freezing bone tissue and bone soft tissue using the freezing solution of the present embodiment is as follows:

[0054] Step 1, preparing the above-mentioned freezing solution;

[0055]Step 2, wash the tissue to be frozen for 3-5 times with phosphate buffered saline, and then incubate in the freezing solution at 4°C for 2 hours;

[0056] Step 3: Gradually lower the temperature of the tissue and cryopreservation solution to -80°C at a rate of -0.3°C...

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Abstract

The invention belongs to the technical field of tissue cryopreservation, and particularly relates to a cryopreservation solution for bone tissue and bone soft tissue and a cryopreservation method. The invention provides a novel cryopreservation solution for bone tissues and bone soft tissues, and also provides a method for cryopreserving the bone tissues and the bone soft tissues by using the cryopreservation solution. The cryopreservation liquid is particularly suitable for cryopreservation of bone tissues and bone soft tissues, can effectively reduce the toxicity of a low-temperature protective agent in the cryopreservation liquid to cells, improve the cell survival rate and reduce the damage to the cells and the tissues, so that the preservation time of in-vitro bone tissues and bone soft tissues is prolonged, and the cryopreservation liquid has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of tissue cryopreservation, and in particular relates to a cryopreservation solution and a cryopreservation method for bone tissue and bone soft tissue. Background technique [0002] In clinical practice, bone defects caused by various reasons such as trauma, infection, and bone tumor resection are one of the difficult problems in orthopedic treatment, and bone transplantation is the preferred treatment method. Although autologous bone grafting is the "gold standard" for repairing bone defects, its application in clinical practice is limited due to the increased trauma to patients caused by bone harvesting and the limited amount of bone harvested. Allograft bone has a wide range of sources, less rejection, no secondary damage, and solves the problem of insufficient source of autologous bone graft tissue, so it is widely used in clinical practice. How to preserve the allogeneic bone and bone soft tissue take...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 黄石书冉力瑜龙丹
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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