Preparation method of composite film added with arginine acidified chitosan and zinc oxide nanoparticles
A technology of argininated chitosan and zinc oxide nano-technology, which is applied in the field of food packaging materials, can solve the problems of limited application, and achieve the effects of increased water contact angle, great application potential and prospects, and decreased water vapor transmission rate
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Embodiment 1
[0031] A method for a composite antibacterial packaging film prepared by adding arginated chitosan and zinc oxide nanoparticles, the specific steps are as follows:
[0032] (1) Weigh 1 g of chitosan and dissolve it in 100 mL of MES buffer (25 mM, PH=5), stir on a magnetic stirrer for 1 h until it is completely dissolved, and the solution is transparent;
[0033] (2) Weigh 2.06 g of arginine and dissolve it in 50 mL of MES buffer, and stir on a magnetic stirrer for 5 min until it is completely dissolved.
[0034] (3) Weigh 3.4 g of 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and 2.04 g of N-hydroxysuccinimide (NHS) into the arginine solution. , stir and activate on a magnetic stirrer for 2h;
[0035] (4) Drop the activated arginine solution into the chitosan solution, and heat it up to 45°C in a magnetic stirrer for 12 hours.
[0036] (5) Weigh 0.5g of arginated chitosan and dissolve it in 50mL of deionized water, heat it up to 70°C in a magnetic stirrer and stir for 1...
Embodiment 2
[0043] A method for a composite antibacterial packaging film prepared by adding arginated chitosan and zinc oxide nanoparticles, the specific steps are as follows:
[0044] (1) Weigh 1 g of chitosan and dissolve it in 100 mL of MES buffer (25 mM, PH=5), stir on a magnetic stirrer for 1 h until it is completely dissolved, and the solution is transparent;
[0045] (2) Weigh 2.06 g of arginine and dissolve it in 50 mL of MES buffer, and stir on a magnetic stirrer for 5 min until it is completely dissolved.
[0046] (3) Weigh 3.4 g of 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and 2.04 g of N-hydroxysuccinimide (NHS) into the arginine solution. , stir and activate on a magnetic stirrer for 2h;
[0047] (4) Drop the activated arginine solution into the chitosan solution, and heat it up to 45°C in a magnetic stirrer for 12 hours.
[0048] (5) Weigh 0.5g of arginated chitosan and dissolve it in 50mL of deionized water, heat it up to 70°C in a magnetic stirrer and stir for 1...
Embodiment 3
[0055] A method for a composite antibacterial packaging film prepared by adding arginated chitosan and zinc oxide nanoparticles, the specific steps are as follows:
[0056] (1) Weigh 1 g of chitosan and dissolve it in 100 mL of MES buffer (25 mM, PH=5), stir on a magnetic stirrer for 1 h until it is completely dissolved, and the solution is transparent;
[0057] (2) Weigh 2.06 g of arginine and dissolve it in 50 mL of MES buffer, and stir on a magnetic stirrer for 5 min until it is completely dissolved.
[0058] (3) Weigh 3.4 g of 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and 2.04 g of N-hydroxysuccinimide (NHS) into the arginine solution. , stir and activate on a magnetic stirrer for 2h;
[0059] (4) Drop the activated arginine solution into the chitosan solution, and heat it up to 45°C in a magnetic stirrer for 12 hours.
[0060] (5) Weigh 0.5g of arginated chitosan and dissolve it in 50mL of deionized water, heat it up to 70°C in a magnetic stirrer and stir for 1...
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