Rapid method for screening efficient rhizobium
A rhizobia, fast technology, applied in the field of microorganisms, can solve the problems of time-consuming and laborious, difficult to obtain strains, etc., to achieve the effect of simplifying the screening process
Pending Publication Date: 2022-05-06
NINGXIA WU FENG AGRI TECH CO LTD +1
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Problems solved by technology
Traditional screening methods require procedures such as isolation, purification, and preservation of rhizobia, which is a very time-consuming and laborious task
Even with such time-consuming and labor-intensive screening, it is difficult to obtain truly excellent strains, and most of the preserved strains are useless, because rhizobia are affected by nitrogen in two links, the first is the nodulation process, and the second is nitrogen fixation process, both of which are feedback-inhibited by nitrogen
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[0030] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0031] The present invention relates to a quick method for screening high-efficiency rhizobia, comprising the following steps:
[0032] S1, collect root nodules, and prepare root nodules into bacterial liquid;
[0033] S2, preparing high-nitrogen nutrient solution and nitrogen-free nutrient solution;
[0034] S3. Cultivate two groups of seedlings respectively with high-nitrogen nutrient solution and nitrogen-free nutrient solutio...
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The invention discloses a rapid method for screening efficient rhizobium, which comprises the following steps: firstly, preparing collected rhizobium into a bacterial liquid, inoculating root nodules to seedlings, and then carrying out nitrogen fixation cultivation; after a period of time, carrying out primary screening, and then carrying out nitrogen-free culture; finally, the best plants are screened out through secondary screening, the largest root nodules are cut off, and surface disinfection, scribing purification and preservation are conducted. According to the method, the screening process of the rhizobium is greatly simplified, and good strains capable of nodulating under the high-nitrogen condition and fixing nitrogen under the nitrogen-free condition can be screened out through the two-step water culture method.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a quick method for screening high-efficiency rhizobia. Background technique [0002] Rhizobia are a special class of nitrogen-fixing bacteria that form root nodules in symbiosis with legumes. Its main function is to convert the nitrogen in the air into the nitrogen nutrient that the host plant can directly absorb, to replace the synthetic nitrogen fertilizer, referred to as nitrogen fixation. The nitrogen fixation efficiency of most rhizobia in soil is relatively low, and some even completely lose nitrogen fixation ability, and only a few rhizobia have high nitrogen fixation efficiency. And these low-efficiency or invalid rhizobia often have a higher survival rate in the soil, so the root nodules on crops are mostly low-efficiency or invalid root nodules under natural conditions. [0003] In order to give full play to the effect of high-efficiency nitrogen-fixing strains...
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IPC IPC(8): C12Q1/04C12N1/02A01G31/00C12R1/41
CPCC12Q1/04C12N1/02A01G31/00Y02P60/21
Inventor 杨国平王明
Owner NINGXIA WU FENG AGRI TECH CO LTD