Amplification module device with lightweight structure

Abstract

The technical problem to be solved by the invention is to provide the amplification module device capable of realizing rapid heating and cooling. According to the technical scheme, the amplification module device of the lightweight structure comprises a module containing part and a heating part; the accommodating module part comprises a module body and a module mask matched with the module body, the module body comprises a bottom plate and a plurality of accommodating units uniformly arranged on the bottom plate, the appearance of each accommodating unit is in a cylindrical shape extending from the bottom plate, a reaction cavity matched with the reaction cup is formed in each accommodating unit, and the accommodating units are connected with the bottom plate; hollow structures are arranged in interval areas between the accommodating units on the bottom plate, and connecting edge parts are formed between the adjacent accommodating units; the module body is covered with the module mask, a hole-shaped structure matched with the open end of the reaction cavity of the containing unit is formed in the module mask, and the space between the module mask and the module body is filled with a heat preservation material.

Description

technical field [0001] The invention relates to the technical field of digital PCR, in particular to an amplification module device with a lightweight structure. Background technique [0002] PCR reaction is one of the main processes of qPCR and digital PCR, and its main process is the process of temperature control change, that is, the process of realizing rapid temperature change. The process mainly includes three temperature sections a. Heating the sample to about 95°C for denaturation, b. Cooling the sample to about 55°C for annealing, c. Heating the sample to about 72°C for extension. The follow-up is to repeat the heating and cooling process of a-c for about 35-40 times. [0003] For the current qPCR, especially digital PCR, due to the use of a larger reaction vessel, the volume of the module will also increase, and the heating and cooling speed will become slower and slower, and the PCR reaction time will also become a constraint on the entire detection process. One...

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Problems solved by technology

[0003] For the current qPCR, especially digital PCR, due to the use of a larger reaction vessel, the volume of the module will also increase, and the heating and cooling speed will become slower and slower, and the PCR reaction time will also become a constraint on the entire detection process. One of the main factors of process time

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