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Immunofluorescence kit for detecting circulating prostate epithelial cells in blood and use method of immunofluorescence kit

A technology of epithelial cells and immunofluorescence, applied in the field of molecular biology, can solve the problems of no kit report, rare circulating cells found in the blood of patients with benign prostatic hyperplasia, etc.

Pending Publication Date: 2022-05-10
珠海中科先进技术研究院有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, it is known that circulating tumor cells exist in the blood of patients with malignant prostate cancer, but there are no reports of circulating rare cells in the blood of patients with benign prostatic hyperplasia, and there are no reports of relevant kits

Method used

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  • Immunofluorescence kit for detecting circulating prostate epithelial cells in blood and use method of immunofluorescence kit
  • Immunofluorescence kit for detecting circulating prostate epithelial cells in blood and use method of immunofluorescence kit
  • Immunofluorescence kit for detecting circulating prostate epithelial cells in blood and use method of immunofluorescence kit

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Embodiment

[0058] The immunofluorescence kit for detecting circulating prostate epithelial cells in blood of this embodiment includes fixative solution, blocking solution, cell membrane permeabilization solution, antibody staining solution, fluorescent dye, DAPI staining solution and mounting solution, wherein the The antibody staining solution is a mixed solution of mouse anti-CK15 antibody and rat anti-CD45 antibody.

[0059] Wherein, the concentration ratio of mouse anti-CK15 antibody and rat anti-CD45 antibody in the antibody staining solution is 1:1-1:3.

[0060] The fixative solution is a PBS solution of paraformaldehyde with a mass percent concentration of 2%, and the pH value is 7.0-7.5.

[0061] The blocking solution is 2% donkey serum in PBS solution.

[0062] The cell membrane permeation solution is a PBS solution of Triton X-100 with a concentration of 0.1% by volume.

[0063] The DAPI staining solution is a 5 μg / ml DAPI solution in PBS.

[0064] The mounting solution is a...

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Abstract

The invention discloses an immunofluorescence kit for detecting circulating prostate epithelial cells in blood and a use method of the immunofluorescence kit, and belongs to the technical field of molecular biology. The immunofluorescence kit for detecting circulating prostate epithelial cells in blood comprises a stationary liquid, a confining liquid, a cell membrane permeation liquid, an antibody staining liquid, a fluorescent dye, a DAPI staining liquid and a mounting liquid. Wherein the antibody staining solution is a mixed solution of a mouse anti-CK15 antibody and a rat anti-CD45 antibody. The invention also discloses a use method of the immunofluorescence kit for detecting circulating prostate epithelial cells in blood. According to the immunofluorescence kit, the combination of the mouse anti-CK15 antibody and the rat anti-CD45 antibody is adopted, prostate epithelial cells dissociating into blood can be screened out with high specificity, and the detection rate of benign prostatic hyperplasia and prostatic cancer is high.

Description

technical field [0001] The invention relates to an immunofluorescence reagent kit for detecting circulating prostate epithelial cells in blood and a using method thereof, belonging to the technical field of molecular biology. Background technique [0002] Prostatic hyperplasia is a common health problem among middle-aged and elderly men. Patients often present symptoms such as frequent urination, urgency, urinary incontinence, dysuria, and hematuria. BPH can be divided into benign hyperplasia and malignant hyperplasia. According to the "Guidelines for the Diagnosis and Treatment of Benign Prostatic Hyperplasia" published in 2011, the incidence of histological benign prostatic hyperplasia increases with age, usually after the age of 40, and the incidence rate of men over 60 years old is greater than 50%. Up to 83% of men suffer from enlarged prostate by the age of 10. [0003] The course of benign prostatic hyperplasia is slow, and there are no obvious symptoms in the early...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N21/64
CPCG01N33/57434G01N21/6428G01N21/6458G01N2021/6439
Inventor 徐畅高飞陈婉玲姜长安
Owner 珠海中科先进技术研究院有限公司
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