Pharmaceutical composition for preventing and treating polycystic ovarian syndrome and application thereof
A technology for polycystic ovary and a composition, applied in the field of medicine, can solve the problem that the effect of polycystic ovary syndrome needs to be improved, and achieve the effect of improving polycystic ovary syndrome and reversing insulin resistance
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Embodiment 1
[0023] Three-week-old SD rats were selected to prepare polycystic ovary syndrome insulin resistance animal models, and drug intervention was performed on the successful model mice.
[0024] ELISA and other methods were used for detection, and statistical software SPSS 22.0 was used for analysis to clarify the synergistic therapeutic effect and possible mechanism of the pharmaceutical composition of the embodiment of the present invention on polycystic ovary syndrome insulin resistance animal models.
[0025] 1. Materials
[0026] 1.1 Experimental animals
[0027] 120 3-week-old SPF female SD rats were raised in the Animal Center of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine. 25°C constant temperature (50% humidity) clean-grade breeding, without feeding vitamin products, 12h light and 12h dark cycle alternately.
[0028] 1.2 Main reagents
[0029] DHEA (Sigma Company), formononetin and isoliquiritigenin were extracted from Shaoya...
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