PBMC (peripheral blood mononuclear cell) separation method for improving tumor killing capability of immune cells
A technology of immune cells and separation methods, which is applied in the field of PBMC separation to improve the ability of immune cells to kill tumors, and can solve problems that do not involve PBMC separation equipment
Active Publication Date: 2022-05-13
SHANDONG UNIV
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[0004] However, the above-mentioned technical solution does not involve the specific method of PBMC separation and the equipment used. Therefore, the application proposes a PBMC separation method that improves the ability of immune cells to kill tumors
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[0039] Example: Please refer to Figure 1 to Figure 8 :
[0040] The present invention proposes a method for separating PBMCs that improves the ability of immune cells to kill tumors. The method for separating PBMCs that improves the ability of immune cells to kill tumors uses medical blood centrifugal equipment to realize the centrifugal treatment involved in the separation process of PBMCs; the medical blood Centrifuge equipment includes:
[0041] Base 1, centrifugal part 2, locking part 3 and auxiliary part 5;
[0042] The base 1 is fixed on the workbench;
[0043] The centrifugal part 2 is composed of a rotating shaft A201, a driving motor 202, a stopper 203, a placement seat 204, a sealing plate 205 and a sealing gasket 206, and the rotating shaft A201 is rotatably connected to the base 1;
[0044] A test tube 4 is placed on the centrifugal part 2;
[0045] The locking part 3 is composed of a limit seat 301, a ball 302, a connecting arm 303, a force block 304, a locki...
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The invention provides a PBMC (peripheral blood mononuclear cell) separation method for improving the tumor killing capability of immune cells. According to the PBMC separation method for improving the tumor killing capability of the immune cells, centrifugal treatment involved in the PBMC separation process is realized by using medical blood centrifugal equipment; the medical blood centrifugal equipment comprises a base, a centrifugal part, a locking part and an auxiliary part, the base is fixed on the workbench; connecting plates are welded to the base, and a strength strengthening structure of the base is formed by the connecting plates. The locking rod is in threaded connection with the base, the head end of the locking rod is polished, and the polished head end of the locking rod is of a hemispherical structure. The head end of the locking rod is in contact with the stress block, so that the sealing between the sealing gasket and the test tube is realized, and the problems that firstly, blood in the test tube is easy to scatter in the rotating process of an existing device; and secondly, an existing device often needs to be fixed to a workbench, but fixing bolts are prone to loosening.
Description
technical field [0001] The invention relates to the technical field of immune medicine, in particular to a PBMC separation method for improving the ability of immune cells to kill tumors. Background technique [0002] Breast cancer is considered to be an immune "cold" tumor. Only a small number of patients are effective to the existing immune checkpoint inhibitors, and the treatment response rate is poor. Therefore, it is urgent to explore new and effective immunotherapy options. [0003] In the previous application CN202210012502.0 of our research team, a new use of gamma-interferon-induced lysosomal sulfhydryl reductase GILT in enhancing T cell-mediated anti-tumor immune response was proposed. The results showed that the high expression of GILT can significantly increase the level of killer T cells in tumor tissue. [0004] However, the above technical solution does not involve the specific method of PBMC separation and the equipment used. Therefore, the present applicati...
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IPC IPC(8): B04B5/04B04B7/00B04B9/02B04B15/00
CPCB04B5/0414B04B15/00B04B7/00B04B9/02
Inventor 余之刚刘丽媛黄淑亚王斐于理想刘现芳
Owner SHANDONG UNIV