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Tumomycorrhiza strain TP-11 with capability of promoting growth of new leaves of dendrobium nobile and application of tumomycorrhiza strain TP-11

A technology of TP-11 and mycorrhizal bacteria, applied in the field of microorganisms, can solve the problems of functional differences of symbiotic bacteria, etc., and achieve the effects of low cost, growth promotion and easy operation

Active Publication Date: 2022-05-17
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are functional differences among different symbiotic bacteria, and there is no report of symbiotic fungi that promote the growth of new leaves of Dendrobium seedlings in the prior art.

Method used

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  • Tumomycorrhiza strain TP-11 with capability of promoting growth of new leaves of dendrobium nobile and application of tumomycorrhiza strain TP-11
  • Tumomycorrhiza strain TP-11 with capability of promoting growth of new leaves of dendrobium nobile and application of tumomycorrhiza strain TP-11

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Isolation, purification and preservation method of strain TP-11

[0035] 1. Ex situ induction of Dendrobium officinale seedling mycorrhizal

[0036] (1) From the end of July to September 2018, the team conducted field research in many places, interviewed local drug collectors, and finally confirmed the Guangnan ( 23 ° 58 ′ N, 105 ° 11 ′ E; 1428m alt.) in Yunnan Province, hunan Kunshan Karst landform (26 ° 30 ′ N, 111 ° 10 ′ E; 340malt.) With Danxia Landform (26°20′N, 110°46′E; 455m alt.), Chongqing Luotian (30°31′N, 108°33′E; 1200malt.), Sichuan Luding (29°23′N, 102°21′E; 1382m alt.) and asbestos (29°22′N, 105°11′E;3596malt.) Six places are dendrobium officinale, because historically herb collectors have collected wild Dendrobium officinale here. During this period, six in situ matrices were collected and brought back to the laboratory, and the matrix brought back from the wild was mixed with the sterile mixed matrix (bark, peat soil, volcanic stone in a 2:1:1 ratio) and ot...

Embodiment 2

[0057] Experiment on the effectiveness of the fungus TP-11 strain of the genus Oncocorrhizae to promote the growth of new leaves in Dendrobium officinale seedlings

[0058] Symbiosis experiments between sterile Dendrobium officinale seedlings and fungi in the medium were used to verify whether the fungus had a promoting effect on new leaf growth of seedlings and compared the differences in the effect of different fungi on new leaf growth:

[0059] (1) Preparation of symbiotic medium: Symbiotic germination medium is oatmeal agar medium, including 4g · L -1 Oats and 10g · L -1 Agar, medium pH of 5.6~5.8;

[0060](2) 8 strains of mycorrhizal fungi for verifying the pro-growth function and the mycorrhizal strain TP-11 of the present invention were removed, inoculated on PDA medium, placed in an artificial climate chamber, and activated in a dark culture at a temperature of 25±2 °C to obtain a symbiotic strain material in a petri dish where the fungal hyphae are overgrown;

[0061] (...

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Abstract

The invention provides a nostomycorrhiza strain TP-11 with a capability of promoting growth of new leaves of dendrobium and application of the nostomycorrhiza strain TP-11, and belongs to the technical field of microorganisms. The invention provides a nostomycorrhiza strain TP-11 capable of promoting the growth of new leaves of dendrobium nobile, and the preservation number of the nostomycorrhiza strain TP-11 is CCTCC (China Center for Type Culture Collection) NO: M 20211284. A plurality of nostomycorrhizal fungi are separated from roots of ex-situ induced dendrobium officinale seedlings and are subjected to symbiotic culture with the dendrobium officinale seedlings respectively, the nostomycorrhizal fungi are used for mycorrhizal treatment of the dendrobium officinale seedlings through the strains, compared with other separated strains, the strain TP-11 can remarkably promote growth of new leaves of the dendrobium officinale seedlings, and the number of the new leaves of the dendrobium officinale seedlings growing for 30 days and 90 days has the remarkable advantage. Dendrobium officinale seedlings and the strain TP-11 show a beneficial symbiotic relationship, by means of symbiosis of the strain TP-11 and the dendrobium officinale seedlings, mycorrhization of the dendrobium officinale seedlings is achieved, and the strain TP-11 can be used as a biological agent raw material for dendrobium officinale cultivation.

Description

Technical field [0001] The present invention belongs to the field of microbial technology, specifically relates to a strain having the ability to promote the growth of dendrobium new leaves mycorrhizal strain TP-11 and its applications. Background [0002] Dendrobium officinale wild resources are mainly distributed in China's Hunan, Yunnan, Guizhou, Sichuan and other karst terrain, like warm and humid climate, suitable for cool, humid, air smooth half yin and half yang environment. Dendrobium officinale has a high ornamental value, as a traditional and valuable Chinese medicinal material in China, it also has a very high medicinal value, with the effect of nourishing yin and clearing heat, benefiting the stomach and strengthening the waist, etc., and is praised by "Daozang" as "the first of the nine immortal herbs in China". However, under natural conditions, its low reproductive rate, coupled with the long-term uncontrolled collection of its wild resources, coupled with the ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01G31/00C12R1/01
CPCC12N1/20A01N63/20A01G31/00
Inventor 高江云吴益花陈德赟李能奇
Owner YUNNAN UNIV
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