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Chronic pain treatment target and application thereof

A technology for chronic pain and therapeutic targets, applied in the field of biomedicine, can solve unknown problems

Active Publication Date: 2022-05-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether ALKBH5 has a role in pain and is involved in pain regulation remains unknown

Method used

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  • Chronic pain treatment target and application thereof
  • Chronic pain treatment target and application thereof
  • Chronic pain treatment target and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 High-throughput sequencing results show differentially highly expressed mRNAs in TG tissues of CCI-ION model rats

[0031] (1) Chronic Compressive Injury of Infraorbital Nerve (CCI-ION) Model and Determination of Mechanical Pain Threshold

[0032] Adult male healthy Sprague-Dawley rats, weighing 180–250 g, were provided by the Experimental Animal Center of Soochow University. The animal center's health-level approval document number: SYXK (Su) 2007-0035.

[0033] Model establishment: Rats were anesthetized by intraperitoneal injection with 4% chloral hydrate at a dose of 1 ml / 100 g. The rats were fixed on the operating table in a supine position, and cut with a sterile blade at the level of the left maxillary first molar. , using a bluntly curved glass rod to carefully dissect the surrounding tissue until the infraorbital nerve is exposed. The two ends are ligated with 5-0 silk thread, the spacing is 2 mm, and the force should not be too large. After the su...

Embodiment 2

[0038] Example 2 Fluorescence quantitative PCR detection of ALKBH5 protein expression in TG tissue of CCI-ION model rats significantly increased

[0039] (1) Establishment of a chronic compressive injury (CCI-ION) model of the infraorbital nerve (same as Example 1).

[0040] (2) RNA extraction and real-time quantitative PCR

[0041] The rat TG was extracted using sterilized instruments and placed in a 1.5 ml sterilized centrifuge tube, 1 ml of Trizol was added, the tissue was broken into a homogenate, and the tissue was placed on ice for 30 minutes. Add 100 microliters of chloroform, and centrifuge in a centrifuge at 4 degrees and 12,000 rpm for 20 minutes. The upper aqueous phase was sucked into a new centrifuge tube, an equal volume of isopropanol was added, and the solution was placed in a refrigerator at -20 degrees for 20 minutes. Centrifuge at 4 degrees in a centrifuge at 12,000 rpm for 15 minutes, discard the supernatant, and add 75% ethanol to wash the precipitate. ...

Embodiment 3

[0044] Example 3 siRNA interference sequence can effectively reduce the expression of ALKBH5 in CCI-ION model rat TG

[0045] (1) Protein extraction (same as Example 2).

[0046] (2) Protein electrophoresis (same as Example 2).

[0047] (3) Stereotaxic injection of siRNA interference sequence in rat TG: the rats were anesthetized and fixed on the brain stereotaxic apparatus, the scalp was cut with a scalpel, and the H 2 O 2 Corrosion of tissue, exposure of coronal and sagittal sutures. The origin was positioned at the point of intersection of the two, namely the bregma: 3 mm posterior to the bregma, 3 mm to the left of the midline, and 11.7 mm below the surface of the skull. ALKBH5 siRNA and control siRNA (purchased from Zima Gene) were injected at a final concentration of 50 μmol / L, each rat was injected with 3 μL, the injection was completed in 5 minutes, and the needle was kept for 10 minutes.

[0048] like image 3 As shown, 28 rats were divided into 4 groups with 7 r...

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Abstract

The invention relates to a chronic pain treatment target, and the treatment target is ALKBH5. The ALKBH5 with high specificity and difference expression in a pain model is provided for the first time, it is found that the chronic pain can be remarkably relieved by reducing the expression of the ALKBH5, it is prompted that the ALKBH5 plays a significant role in occurrence and development of the chronic pain, and new possibility is provided for research, prevention and treatment of chronic pain drugs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a chronic pain treatment target and application thereof. Background technique [0002] Chronic pain (such as trigeminal neuralgia, sciatica, cancer pain, migraine, etc.) is a chronic disease that seriously endangers the physical and mental health and quality of life of human beings. The characteristics make patients suffer for a long time, and can induce malignant emotions or mental disorders such as anxiety, depression and fear, and even cause individuals to have suicidal tendencies, seriously endangering patients' lives and quality of life. [0003] At present, the research on chronic pain treatment drugs has been very extensive. For example, Cao Xiaopan treated trigeminal neuralgia with baclofen combined with adenosylcobalamin (the effect of baclofen combined with adenosylcobalamin in the treatment of trigeminal neuralgia [J]. Chinese Modern Doctors) , 2021, 59(6): 42-44,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886A61K31/713A61P25/02A61P35/00A61P29/00A61P25/06
CPCC12Q1/6883C12Q1/6886A61K31/713A61P25/02A61P35/00A61P29/00A61P25/06C12Q2600/158C12Q2600/136
Inventor 陶金黄紫彤张园
Owner SUZHOU UNIV
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