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Primer and probe composition and method for rapidly detecting aeromonas hydrophila through RPA-LFS

A technology of Aeromonas hydrophila and composition, which is applied in the field of primer and probe composition for rapid detection of Aeromonas hydrophila by RPA-LFS, which can solve the problem of long detection time and inconvenient operation of Aeromonas hydrophila , large interference and other problems, to achieve the effect of getting rid of instrument dependence, easy operation and high sensitivity

Pending Publication Date: 2022-05-27
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the detection of Aeromonas hydrophila takes a long time in the prior art, the operation is inconvenient, false positives are prone to occur and the problems are greatly interfered by other factors

Method used

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  • Primer and probe composition and method for rapidly detecting aeromonas hydrophila through RPA-LFS
  • Primer and probe composition and method for rapidly detecting aeromonas hydrophila through RPA-LFS
  • Primer and probe composition and method for rapidly detecting aeromonas hydrophila through RPA-LFS

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Embodiment Construction

[0024] The following will be combined with the embodiments of the present invention to describe the technical solution of the present invention clearly and completely, it is clear that the embodiments described are only part of the embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is in fact merely illustrative and in no way is intended as any restriction on the present invention and its application or use. Based on embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative labor, are within the scope of protection of the present invention.

[0025] Table 1 Nucleic acid sequences as involved in the present invention

[0026]

[0027] Table 2 Modified nucleic acid sequences

[0028]

[0029] Step 1. Strain source

[0030] Mild aeromonas (ATCC 43979), Aeromonas guinea pig (ATCC 15468), Aeromonas viriz (ATCC35624), and Aeromonas aeromonas...

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Abstract

The invention relates to the technical field of molecular diagnosis, in particular to a primer and probe composition and a method for rapidly detecting aeromonas hydrophila through RPA-LFS. The composition comprises a forward primer as shown in SEDQ ID NO. 1, a reverse primer as shown in SEDQ ID NO. 2 and a probe as shown in SEDQ ID NO. 3. According to the primer and probe composition and the detection method, the aeromonas hydrophila can be accurately detected, no false positive result exists, the specificity is high, the sensitivity is high, and the detection is rapid.

Description

Technical field [0001] The present invention relates to the field of molecular diagnostic techniques, particularly to an RPA-LFS rapid detection of primers and probe compositions and methods of Aeromonas hydrophilus. Background [0002] Prior art in the biochemical culture method is the national standard for the detection of Aeromonas hydrophila, the method is complex to operate, first of all, the need for strain culture, and then through biochemical tests to identify water-based aeromonas and their pathogenicity, the detection time is more than five days, often leading to the deterioration and spread of the disease. [0003] Pollard et al. established a PCR method based on the aer gene of Aeromonas hydrophila. Griffin et al. established a qPCR method for detecting Aeromonas hydrophila. PCR and qPCR are fast, specific, and sensitive nucleic acid amplification techniques that require expensive thermal cycling equipment and professionals, are generally suitable in laboratories, and...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6844C12Q1/04C12N15/11
CPCC12Q1/689C12Q1/6844C12Q2531/119C12Q2521/507C12Q2522/101C12Q2563/107C12Q2537/1376C12Q2565/625Y02A50/30
Inventor 高嵩曾丽媚余泓张迪王昱
Owner JIANGSU OCEAN UNIV
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