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Method for simply and rapidly determining lignin content in lignocellulose biomass

A technology for rapid determination of lignocellulose, which is used in measurement devices, preparation of test samples, color/spectral property measurement, etc. easy effect

Pending Publication Date: 2022-05-27
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, furfural products derived from xylan interfere with lignin's UV absorption at 280nm, and AcBr lignin values ​​are often not the same as the Klason lignin assay, which is still considered to provide the best lignin measurement

Method used

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  • Method for simply and rapidly determining lignin content in lignocellulose biomass
  • Method for simply and rapidly determining lignin content in lignocellulose biomass
  • Method for simply and rapidly determining lignin content in lignocellulose biomass

Examples

Experimental program
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Embodiment 1

[0037] Weigh 5 mg of enzymatically hydrolyzed lignin and add it to 1 mL of [Emim][OAc] ionic liquid to dissolve. Dilute methyl sulfoxide (DMSO) 100-500 times to obtain a completely dissolved standard solution, add 3 mL of the standard solution to the cuvette, and then place it in a UV spectrophotometer to obtain the UV absorption curve. The maximum value obtained at 285nm is used as a standard curve, and the relevant results are as follows figure 1 As shown, the UV absorption coefficient ε of lignin at 285 nm was obtained according to the standard curve. The ε value is a fixed value, which can be used directly in other testing processes.

Embodiment 2

[0039] A method for simple and rapid determination of lignin content in lignocellulosic biomass using ionic liquid, the steps are as follows:

[0040] (1) The lignocellulosic biomass is ball-milled with a ball mill, and the ball-milling conditions are room temperature, rotating speed of 500-1000rpm, ball-milling for 2-4h, and then vacuum drying;

[0041] (2) Weigh 10-50 mg of the sample obtained in step (1) and dissolve it in 1 mL of [Emim][OAc] ionic liquid system. Completely dissolved sample solution;

[0042] (3) dilute the sample solution obtained in step (2) by 100-200 times with dimethyl sulfoxide, and control the ultraviolet absorbance value of the diluted sample solution at a wavelength of 285 nm to be 0.2-1;

[0043] (4) Substitute the ultraviolet absorption photometric value into the aforementioned lignin calculation formula to calculate the lignin content C Lignin (%).

Embodiment 3

[0045] According to the steps of Example 2, 5-50 mg of corn stalks with different particle sizes were weighed and added to 1 mL of [Emim][OAc] ionic liquid to dissolve. The sample was diluted 200 times with dimethyl sulfoxide (DMSO) to obtain a completely dissolved sample solution to be tested, 3 mL of the sample solution to be tested was added to a cuvette and then placed in a UV spectrophotometer to obtain UV absorption Curve, take the UV absorption value at 285nm, and substitute it into the lignin calculation formula to obtain the effect of different dissolution temperature, time, particle size and quality on the dissolution of lignin.

[0046] The results are as figure 2 As shown, this example fully illustrates that, for the influence of different dissolution temperature, dissolution time, biomass particle size and quality on the UV absorption photometric value of biomass after dissolving in [Emim][OAc] ionic liquid, it is determined that the best method of the present in...

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Abstract

The invention discloses a method for simply and rapidly determining the content of lignin in lignocellulose biomass, which comprises the following steps: drying enzymatic hydrolysis lignin, grinding into fine powder, dissolving in [Emim] [OAc] ionic liquid, adding dimethyl sulfoxide for dilution to prepare a standard sample solution, determining the absorbance at 285 nm by using an ultraviolet spectrophotometer, and determining the content of lignin in the lignocellulose biomass according to the absorbance. Making a standard curve of absorbance and lignin concentration to obtain an ultraviolet absorption coefficient epsilon; the method comprises the following steps: grinding lignocellulose biomass, drying the lignocellulose biomass, dissolving the lignocellulose biomass in a [Emim] [OAc] ionic liquid system, adding a diluent for dilution, and controlling the ultraviolet absorbance value of a diluted to-be-detected sample solution under ultraviolet light with the wavelength of 285 nm to be 0.2-1; and substituting the ultraviolet absorbance value into a formula for calculation. The method disclosed by the invention has the characteristics of simplicity and convenience in operation, few procedures, easily available reagents, environment-friendly process, high sensitivity, few required samples, wide application range and more accurate measurement result.

Description

technical field [0001] The invention relates to a method for determining the lignin content in lignocellulosic biomass, in particular to a simple and rapid method for determining the lignin content in lignocellulosic biomass. Background technique [0002] Lignin is the most abundant aromatic polymer and the second most renewable raw material after cellulose. In plant cells, lignin is cross-linked with hemicellulose and bound into cellulose microfibrils, a major obstacle to its utilization into other biofuels and other bio-based chemicals. Therefore, before developing and utilizing the lignin in a certain lignocellulosic biomass, it is particularly important to measure the lignin content in it. [0003] With the advancement of science and technology, lignin measurement methods are also constantly improving, and the determination of lignin content has expanded from traditional gravimetric analysis to rapid non-destructive testing using various instruments (UV, FTIR, NMR, NIR ...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28G01N1/38
CPCG01N21/33G01N1/286G01N1/38G01N2001/2866Y02P20/54
Inventor 徐芹芹谢海波陈金晖
Owner GUIZHOU UNIV