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Acute rapid separation method for spinal cord scar tissue cells

A technology of tissue cells and separation methods, which is applied in the field of acute and rapid separation of spinal cord scar tissue cells, can solve the problems of cumbersome operation, low efficiency of cell separation and extraction, and high technical requirements, and achieve broad application prospects, improve acquisition efficiency, and small cell damage. Effect

Active Publication Date: 2022-05-31
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can separate a single cell from a tissue block, but the operation is cumbersome, the technical requirements for the operator are also high, and the efficiency of cell separation and extraction is low

Method used

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  • Acute rapid separation method for spinal cord scar tissue cells
  • Acute rapid separation method for spinal cord scar tissue cells
  • Acute rapid separation method for spinal cord scar tissue cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] In this example, a rat thoracic 10-segment spinal cord contusion model was established in a sterile environment, and 4 days after the operation, the following steps were used to cultivate:

[0034] S1: Sterilize all the experimental utensils to be used at high temperature and autoclave, and sterilize them under the ultra-clean bench UV lamp for 30min, for use; prepare high-glucose DMEM and PBS (containing 2% fetal bovine serum and 2% double antibody) After the spinal cord injury model rats were euthanized, the back skin was shaved, disinfected with iodine or alcohol, and a median incision was made along the skin of the back injury area, and the muscles were bluntly separated, taking the injury site as the In the center, cut the spine at 1 cm above and below, and cut off the ribs on both sides at the same time, and remove the spine together with the spine within 2 minutes, that is, separate the tissue block, and put the whole tissue block into the ice bath containing 10% ...

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Abstract

The invention relates to the technical field of cell detection, and discloses a spinal cord scar tissue cell acute rapid separation method, which comprises the following steps: obtaining a tissue block, cleaning, obtaining a spinal cord tissue block, digesting and collecting a cell suspension. The method can greatly reduce the loss of the stromal cells in the scar, effectively shortens the separation time of the stromal cells, is small in damage to the cells, can stably carry out various detections, is simple and convenient to operate, is high in practicability, and has a wide application prospect in the aspects of acute separation and application of the stromal cells and the like. The method is not only suitable for acute separation of spinal cord scar tissue cells, but also suitable for acute separation or primary cell culture of scar tissue cells at other parts.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a method for acute and rapid separation of spinal cord scar tissue cells. Background technique [0002] After spinal cord injury, scars will form in the injured area. In the past, it was believed that although scar tissue seals and fills the injured area, it will hinder axonal regeneration. With the deepening of the current research, it is found that the scar composition is complex, and its formation is a complex multicellular interaction process, and finally a penumbra (glial scar) composed of hyperactivated astrocytes is formed in the injured area. And by a variety of stromal cells (oligodendrocyte precursor cells, pial-derived fibroblasts, vascular-derived fibroblasts, pericytes, ependymal cells and macrophages, etc.) and their secreted matrix components (Collagen matrix, fibronectin and various proteoglycans, etc.) composed of scar center (fibrous scar). Fibrous scar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079
CPCC12N5/0618C12N2509/00
Inventor 徐杨
Owner WEST CHINA HOSPITAL SICHUAN UNIV