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Establishment method of Xiaozhi decoction prescription fingerprint based on Shaoyang prescription syndrome

A technology of Xiao Chai Hu Tang and fingerprints, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of increased time cost, complicated operation, long preparation time, etc. Effect

Active Publication Date: 2022-05-31
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the research of the inventors, the establishment of fingerprints of different Xiaochaihu decoctions needs to select different mobile phases, chromatographic columns and detection wavelengths for detection. When it is necessary to simultaneously establish fingerprints of different Xiaochaihu decoctions, the operation is cumbersome and the preparation time Longer, greatly increasing the time cost

Method used

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  • Establishment method of Xiaozhi decoction prescription fingerprint based on Shaoyang prescription syndrome
  • Establishment method of Xiaozhi decoction prescription fingerprint based on Shaoyang prescription syndrome
  • Establishment method of Xiaozhi decoction prescription fingerprint based on Shaoyang prescription syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Establishment of HPLC fingerprint of Dachaihu Decoction.

[0082] Preparation of Da Chai Hu Decoction test solution: according to the original formula of Shang Han Lun: Bupleurum 24g, Scutellaria baicalensis 9g, Paeoniae 9g, Pinellia 12g, Ginger 15g, Citrus aurantium 9g (burned), Jujube (broken) 12 pieces, rhubarb 6g. Add 2400mL of water, boil over low heat to get 1200mL, remove the medicinal residues, and boil to 600mL.

[0083] Preparation of the test solution of single herbal medicines: take 24g of Bupleurum, 9g of Scutellaria, 9g of Peony, 12g of Pinellia, 15g of Ginger, 9g of Citrus aurantium (burnt), 12 pieces of jujube (broken), and 6g of rhubarb, according to the above method Prepare the test solution of single medicinal material.

[0084] Chromatographic condition optimization:

[0085] Detection wavelength: DAD collection wavelength is 190-640nm, compare the HPLC charts of different wavelengths, finally choose 254nm and 276nm, and select the ELSD spectrum a...

Embodiment 2

[0097] Establishment of HPLC fingerprint of Xiaochaihu Decoction.

[0098] Preparation of the test solution of Xiaochaihu Decoction: Bupleurum 24g, Scutellaria baicalensis 9g, Ginseng 9g, Pinellia 12g, licorice (burnt), ginger (cut) 9g each, jujube (broken) 12 pieces. Add 2400mL of water, boil over low heat to get 1200mL, remove the medicinal residues, and boil to 600mL.

[0099] Preparation of the test solution of single medicinal materials: take 24g Bupleurum, 9g Scutellaria, 9g Ginseng, 12g Pinellia, 9g each of Licorice (burned), 9g of Ginger (cut), and 12 pieces of Jujube (broken), and prepare them according to the above method. Become the test solution of single medicinal material.

[0100] Detection wavelength: DAD collection wavelength is 190-640nm, compare the HPLC charts of different wavelengths, finally choose 254nm and 276nm, and select the ELSD spectrum at the same time. Chromatograms such as Figure 8 As shown, it can be seen that at the wavelengths of 254nm an...

Embodiment 3

[0111] Establishment of HPLC fingerprint of Bupleurum Glauber's Salt Decoction.

[0112] Bupleurum and Mirabilite Decoction Preparation of the test solution: Bupleurum 24g, Scutellaria baicalensis 9g, Ginseng 9g, Pinellia 12g, Radix Glycyrrhiza 9g, Ginger 9g, Jujube (broken) 4 pieces, Glauber's salt 12g. Add 2400mL of water except Glauber's salt, boil 1200mL on low heat, remove the medicine residue, boil to 600mL, add Glauber's salt.

[0113] Preparation of the test solution of single medicinal materials: take 24g of Bupleurum baicalensis, 9g of baicalin, 9g of ginseng, 12g of pinellia, 9g of roasted licorice, 9g of ginger, and 4 pieces of jujube (broken), and make single medicinal materials for use according to the above method. Test solution.

[0114] Detection wavelength: DAD collection wavelength is 190-640nm, compare the HPLC charts of different wavelengths, finally choose 254nm and 276nm, and select the ELSD spectrum at the same time. Chromatograms such as Figure 15 ...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine detection, and relates to a method for establishing a Xiaozhi decoction prescription fingerprint spectrum based on a Shaoyang prescription. The method comprises the following steps: preparing a test solution from a common goldenrop decoction formula, detecting the test solution by adopting high performance liquid chromatography, and establishing a fingerprint spectrum by utilizing a detection spectrogram; in the high performance liquid chromatography detection process, the mobile phase is acetonitrile-0. 19-0.21% formic acid water, the chromatographic column is a waters C18 column, and the detection wavelengths are 253-255 nm and 275-277 nm. The research finds that different common goldenrop soup prescriptions can be simultaneously subjected to high performance liquid chromatography detection by adopting the same mobile phase, chromatographic column and detection wavelength, the easy preparation of the mobile phase, the replacement of the chromatographic column and the input of the detection wavelength before detection are reduced, and the preparation time for detecting different common goldenrop soup prescriptions is greatly saved.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and relates to a method for establishing fingerprints of Xiaochaihu Decoction-like prescriptions based on Shaoyang disease prescription syndrome. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Xiao Chaihu Decoction based on Shaoyang disease prescriptions include Da Chaihu Decoction, Xiao Chaihu Decoction, Chaihu Plus Glauber’s Salt Decoction, Chaihu Guizhi Decoction, Chaihu Longgu Oyster Decoction, etc. According to the research of the inventors, the establishment of fingerprints of different Xiaochaihu decoctions needs to select different mobile phases, chromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/86G01N30/74
CPCG01N30/06G01N30/8686G01N30/74
Inventor 孙蓉王岱杰黄娜娜刘闰平李晓骄阳吴恺怿钟颖
Owner SHANDONG UNIV
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