Unlock instant, AI-driven research and patent intelligence for your innovation.

Low-pH antigen repair buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of low-pH antigen repair buffer solution

A technology of immunohistochemistry and buffer, which is applied in the field of automatic immunohistochemistry staining machine, can solve the problems of being volatile, antigen retrieval buffer cannot be stored and used stably for a long time, and cannot completely cover tissue slides, etc. Stable value, excellent retarding performance and stability performance, long-term stable storage and use effect

Pending Publication Date: 2022-06-07
浙江莱阅病理诊断科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Antigen retrieval solutions currently on the market cannot effectively control the growth of microorganisms in in vitro diagnostic reagents, so that antigen retrieval buffers cannot be stored and used stably for a long time under refrigerated conditions or at room temperature; The combination rate of the key enzymes in the tissue and the substrate is affected; in addition, when the existing antigen retrieval solution is used in a small amount, the phenomenon of liquid inhomogeneity on the slide is prone to occur, and the tissue slide cannot be completely covered, that is, the infiltration is poor; in addition, antigen retrieval When the liquid is used in a small amount, it is prone to dry flakes at high temperature (about 100°C), that is, it is volatile

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-pH antigen repair buffer solution adaptive to full-automatic immunohistochemical staining machine and preparation method of low-pH antigen repair buffer solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A low pH antigen retrieval buffer: each 1000mL low pH antigen retrieval buffer includes: trisodium citrate 2.10g, Tween-20 0.35mL, glycerol 150mL, ProClinTM 950 0.25mL and the balance of purified water, pH Values ​​range from pH 5.8 to 6.2.

[0031] Preparation method: weigh trisodium citrate according to the proportion, dissolve trisodium citrate with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClinTM 950 after dissolving, and finally use purified water Set the volume to the rated volume, and adjust the pH of the antigen retrieval buffer with 1M NaOH solution or HCl solution so that the pH value is in the range of pH 5.8 to 6.2.

Embodiment 2

[0033] A low pH antigen retrieval buffer: each 1000mL low pH antigen retrieval buffer includes: trisodium citrate 2.50g, Tween-200.45mL, glycerol 300mL, ProClinTM 9500.4mL and the balance of purified water, pH range It is pH5.8~6.2.

[0034]Preparation method: weigh trisodium citrate according to the proportion, dissolve trisodium citrate with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClinTM 950 after dissolving, and finally use purified water Set the volume to the rated volume, and adjust the pH of the antigen retrieval buffer with 1M NaOH solution or HCl solution so that the pH value is in the range of pH 5.8 to 6.2.

Embodiment 3

[0036] A low pH antigen retrieval buffer: each 1000mL low pH antigen retrieval buffer includes: trisodium citrate 3.25g, Tween-200.6mL, glycerol 550mL, ProClinTM 950 0.5mL and the balance of purified water, pH value The range is pH5.8~6.2.

[0037] Preparation method: weigh trisodium citrate according to the proportion, dissolve trisodium citrate with purified water, add glycerol in the formula amount, add Tween-20 after complete dissolution, add ProClinTM 950 after dissolving, and finally use purified water Set the volume to the rated volume, and adjust the pH of the antigen retrieval buffer with 1M NaOH solution or HCl solution so that the pH value is in the range of pH 5.8 to 6.2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a low-pH antigen repair buffer solution adapted to a full-automatic immunohistochemical staining machine and a preparation method thereof, and is characterized in that the raw materials of the low-pH antigen repair buffer solution mainly comprise trisodium citrate, Tween-20, glycerol and ProClin950, and the pH value range of the antigen repair buffer solution is 5.8-6.2. According to the scheme, the growth of microorganisms in the in-vitro diagnostic reagent is effectively controlled, so that the antigen repair buffer solution can be stably stored and used for a long time under a cold storage condition or a room temperature condition, has better wettability, is not easy to volatilize at a relatively high temperature, avoids the drying condition of tissue slices, and improves the service life of the tissue slices. The pH value of the antibacterial preservative has the advantages of better stability and better antibacterial preservation function.

Description

technical field [0001] The present application relates to the technical field of automatic immunohistochemical staining machines, in particular to a low pH (pH 5.8-6.2) antigen retrieval buffer adapted to automatic immunohistochemical staining machines and a preparation method thereof. [0002] technical background [0003] Immunohistochemical technology is a detection technology that has developed rapidly in pathological diagnosis in recent years. As an important auxiliary pathological diagnosis method, it has been paid more and more attention in clinical application. At first, the immunohistochemical staining technology was mainly manual operation, the amount of antigen retrieval solution used was large, the method of use was not uniform, the quality standards could not be unified, and the stability of staining results was poor. [0004] Immunohistochemical technology has developed rapidly in recent years, and with the application and promotion of automatic immunohistochemi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N1/30
CPCG01N33/531G01N1/30
Inventor 沈雯徐秀芬
Owner 浙江莱阅病理诊断科技有限公司