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Nano-enzyme with multistage enzyme-linked reaction performance as well as preparation method and application of nano-enzyme

An enzyme-linked reaction and nano-enzyme technology, which is applied in the direction of botany equipment and methods, applications, chemical instruments and methods, etc., can solve the problems of high production cost and poor stability, and achieve low toxicity, good biocompatibility, and preparation The effect of simple method

Pending Publication Date: 2022-06-28
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of using GOx and HRP to realize this cascade reaction is that they are less stable as natural protein enzymes and the production cost is higher
Some reported artificial enzymes have the function of GOx or HRP, but there are few reports of artificial enzymes with both GOx and HRP functions

Method used

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  • Nano-enzyme with multistage enzyme-linked reaction performance as well as preparation method and application of nano-enzyme
  • Nano-enzyme with multistage enzyme-linked reaction performance as well as preparation method and application of nano-enzyme
  • Nano-enzyme with multistage enzyme-linked reaction performance as well as preparation method and application of nano-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Step 1, block g-C 3 N 4 Preparation: put 6g of dicyandiamide into a ceramic calcining boat, heat up from 20°C to 550°C at a constant rate within 4 hours in an air atmosphere, and keep it in an air atmosphere for 4 hours; after the insulation, it is naturally cooled to room temperature and ground to obtain Block g-C 3 N 4 ;

[0043] Step 2, g-C 3 N 4 Preparation of nanosheets: 3 g bulk g-C 3 N 4 Disperse in 30mL anhydrous H 2 SO 4 Stir for 1 hour in medium to obtain g-C 3 N 4 / H 2 SO 4 mixture; gradually add 10 mL of deionized water to g-C 3 N 4 / H 2 SO 4 In the mixture, a clear solution was obtained. The clear solution was then added to 90 mL of absolute ethanol and stirred for 18 hours, and dialyzed to neutrality in deionized water with a dialysis bag (membrane cutoff: 3500 kDa) (to remove residual SO). 4 2- and ethanol), collected after drying at 60 °C to obtain g-C 3 N 4 Nanosheets (g-C 3 N 4 NS);

[0044] Step 3, Nanozyme (Cu / g-C 3 N 4 ) p...

Embodiment 2

[0054] Step 1, block g-C 3 N 4 Preparation: put 6g of dicyandiamide into a ceramic calcining boat, heat up from 20°C to 500°C at a constant rate within 3 hours in an air atmosphere, and keep it in an air atmosphere for 5 hours; after the insulation, it is naturally cooled to room temperature and ground to obtain Block g-C 3 N 4 ;

[0055] Step 2, g-C 3 N 4 Preparation of nanosheets: 3 g bulk g-C 3 N 4 Disperse in 30mL anhydrous H 2 SO 4 Stir for 1 hour in medium to obtain g-C 3 N 4 / H 2 SO 4 mixture; gradually add 10 mL of deionized water to g-C 3 N 4 / H 2 SO 4 In the mixture, a clear solution was obtained. The clear solution was then added to 120 mL of absolute ethanol and stirred for 16 hours, and dialyzed to neutrality (to remove residual SO) in deionized water with a dialysis bag (membrane cutoff: 3500 kDa). 4 2- and ethanol), dried at 50 °C and collected to obtain g-C 3 N 4 Nanosheets (g-C 3 N 4 NS);

[0056] Step 3, Nanozyme (Cu / g-C 3 N 4 ) pre...

Embodiment 3

[0059] Step 1, block g-C 3 N 4 Preparation: put 6g of dicyandiamide into a ceramic calcining boat, heat up from 20°C to 600°C at a constant rate within 5 hours in an air atmosphere, and keep it in an air atmosphere for 3 hours; after the insulation, it is naturally cooled to room temperature and ground to obtain Block g-C 3 N 4 ;

[0060] Step 2, g-C 3 N 4 Preparation of nanosheets: 3 g bulk g-C 3 N 4 Disperse in 30mL anhydrous H 2 SO 4 Stir for 1 hour in medium to obtain g-C 3 N 4 / H 2 SO 4 mixture; gradually add 10 mL of deionized water to g-C 3 N 4 / H 2 SO 4 In the mixture, a clear solution was obtained. The clear solution was then added to 105 mL of absolute ethanol and stirred for 20 hours, and dialyzed to neutrality (to remove residual SO) in deionized water with a dialysis bag (membrane cutoff: 3500 kDa). 4 2- and ethanol), collected after drying at 55 °C to obtain g-C 3 N 4 Nanosheets (g-C 3 N 4 NS);

[0061] Step 3, Nanozyme (Cu / g-C 3 N 4 ) ...

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Abstract

The invention discloses a nano-enzyme with multistage enzyme-linked reaction performance as well as a preparation method and application thereof, and relates to the technical field of medical materials. The nano enzyme is Cu / g-C3N4, and is obtained by modifying a g-C3N4 nanosheet with Cu. The preparation method comprises the following steps: adding a Cu metal precursor and a g-C3N4 nanosheet into water, uniformly mixing and stirring, and drying to obtain a mixture; and sequentially calcining, cooling, washing and drying the mixture in an inert atmosphere to obtain the nano-enzyme with the multi-stage enzyme-linked reaction performance. According to the invention, cascade reaction of enzyme is realized, and the enzyme has effective bactericidal performance. The material research range of the nano-enzyme is expanded, the application field of the g-C3N4 nano-material is expanded, and a novel multifunctional nano-enzyme material is provided.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a nano-enzyme with multi-stage enzyme-linked reaction performance and a preparation method and application thereof. Background technique [0002] Nanozymes have high efficiency and unique biocatalytic activity, and have the characteristics of high catalytic efficiency, stability, economy and large-scale preparation. They have been widely studied in the fields of sterilization, tissue repair, tumor treatment, and biological detection. Nanomaterial-derived artificial enzymes have been extensively studied to mimic and replace protein enzymes. Glucose oxidase (GOx) and horseradish peroxidase (HRP) are commonly used in enzymatic cascade catalytic reactions and can be used for blood glucose monitoring in humans. The principle is that GOx can oxidize glucose to H 2 O 2 and gluconic acid, H 2 O 2 Under the action of HRP, it reacts with a chromogenic substrate, and the gluco...

Claims

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Application Information

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IPC IPC(8): B01J27/24B01J23/72B01J35/00A01N59/20A01N59/00A01P1/00C01B21/082
CPCB01J27/24B01J23/72A01N59/20A01N59/00C01B21/0605C01P2002/84C01P2004/04B01J35/39
Inventor 刘勇伍凡李圆凤王娅然詹益周
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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