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ELISA kit for detecting anti-NXP2 antibody and detection method and application thereof

A detection method and a kit technology, which are applied in the field of ELISA kits for detecting anti-NXP2 antibodies, and achieve the effects of high sensitivity, large detection throughput, and easy promotion and application

Pending Publication Date: 2022-07-05
SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there is no marketable anti-NXP2 antibody detection kit on the market at present. Therefore, in view of the importance of detecting anti-NXP2 antibody, it is necessary to develop a widely applicable, efficient and sensitive anti-NXP2 antibody detection kit and corresponding detection methods. It is particularly important to predict the evolution of the disease and guide clinical treatment

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  • ELISA kit for detecting anti-NXP2 antibody and detection method and application thereof
  • ELISA kit for detecting anti-NXP2 antibody and detection method and application thereof
  • ELISA kit for detecting anti-NXP2 antibody and detection method and application thereof

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Embodiment Construction

[0053] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below through the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the present invention, and not to limit the scope of the present invention. Also, in the following structures, descriptions of well-known structures and techniques are omitted to avoid unnecessarily obscuring the concepts of the present invention.

[0054] The normal temperature referred to in the present invention is the normal temperature generally understood in the art, and the range is 20-25 degrees Celsius.

[0055] 1. Due to the large structure of NXP2 protein and many amino acid sequences, the inventor first analyzed its full-length structure through protein dynamics analysis to obtain its dominant antigenic structural epitope:

[0056] The full-...

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Abstract

The invention discloses an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-NXP2 antibody, which is characterized by comprising a purified dominant antigen epitope NXP2 protein, and an amino acid sequence corresponding to the purified dominant antigen epitope NXP2 protein is shown as SEQ NO.1. The invention also discloses a kit for detecting the anti-NXP2 antibody. The invention also discloses a detection method and application thereof. The kit disclosed by the invention can be used for detecting the anti-NXP2 antibody, makes up the blank of an anti-NXP2 antibody detection method, has the characteristics of large detection flux, exact result, high sensitivity and simplicity and convenience in operation, and is easier to popularize and apply.

Description

technical field [0001] The invention relates to the field of antibody detection, in particular to an ELISA kit for detecting anti-NXP2 antibodies and a detection method thereof. Background technique [0002] Dermatomyositis (DM) is a common life-threatening inflammatory myopathy in clinical practice, mainly involving the skin and muscles. [0003] It is currently considered to belong to the category of autoimmune diseases. The annual incidence of dermatomyositis is about 0.5-1 / 100,000 people, and it is on the rise. [0004] NXP2 is called nuclear matrix protein 2 (nuclear matrix protein 2), also known as MJ or MORC3. Anti-NXP2 antibodies are found in 20%-25% of patients with juvenile dermatomyositis, and the prevalence in adults with dermatomyositis is approximately 1.6%-30%. [0005] The most typical rash in NXP2 antibody-positive patients is cutaneous calcification and peripheral edema, often with frequent and severe myalgias and neck and distal extremity weakness (as o...

Claims

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Application Information

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IPC IPC(8): G01N33/564G01N33/58G01N33/543C07K14/47
CPCG01N33/564G01N33/581G01N33/54393C07K14/47G01N2333/47G01N2800/20G01N2800/10G01N2800/7095
Inventor 赵江峰王楷文曹励欧叶霜
Owner SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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