Closed and sealed conveying system after sampling for TRPC6 nucleic acid amplification detection

Abstract

The invention discloses a sealed conveying system after sampling for TRPC6 nucleic acid amplification detection, which comprises a conveying box, a sealing strip is fixedly mounted in the conveying box, a sealing cover is rotatably mounted on the conveying box, a heat preservation layer is fixedly mounted on the sealing cover, a base is fixedly mounted in the conveying box, a supporting gear is rotatably mounted on the base, and a sealing ring is fixedly mounted on the supporting gear. A supporting gear is arranged in the conveying box, a transfer rack is meshed with the supporting gear, a closed kit structure is installed on the transfer rack through a socket with a clamping block, a kit uncovering structure is installed in the conveying box, the kit uncovering structure is connected to an inhibitor dripping structure through a pull rope, and the pull rope is movably installed in a steering rope sling. According to the closed conveying system, the conveying box with a sealing mechanism and a freezing mechanism is used as a storage and conveying device for RNA samples, independent inhibitor adding at the indoor temperature is not needed, the operation time difference is effectively eliminated, it is guaranteed that the samples are always in the low-temperature environment, and degradation is avoided.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a post-sampling airtight and sealed delivery system for TRPC6 nucleic acid amplification detection. Background technique [0002] Alzheimer's disease is a progressive neurodegenerative disease with an insidious onset. Clinically, it is characterized by generalized dementia such as memory impairment, aphasia, apraxia, agnosia, visual-spatial skills impairment, executive dysfunction, and personality and behavioral changes. Diagnosis is carried out by multiple means such as testing, neuroimaging, electrophysiological testing and genetic testing. Studies have shown that in the human brain, TRPC6 reduces neurotoxicity in Alzheimer's disease by competitively binding to β-amyloid precursor protein (APP), blocking its binding to γ-secretase The formation of the molecule β-amyloid (Aβ). In the peripheral blood of AD patients, the transcriptional mRNA level of TRPC6 gene is de...

AI Technical Summary

Problems solved by technology

However, during operation, after the RNA sample is extracted, it is often necessary to add the enzyme inhibitor dropwise at room temperature. There is a time difference between the steps of extraction and low-temperature storage, which may easily cause sample degradation, and is now used for RNA sample storage and transportation. The transportation system also has slow cooling and uneven cooling, which makes it take a long time for the sample to reach the low temperature, which intensifies the degradation of the sample

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