Methane reduction agent, preparation method and methane in-situ reduction method
A technology of methane and methane oxidizing bacteria, which is applied in the fields of methane reducing agent, preparation, and methane in-situ reduction, which can solve the problems of poor methane removal effect, achieve the effect of reducing the greenhouse effect, wide application range, and broad market application prospects
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Embodiment 1
[0025] A kind of methane in-situ reducing agent, its preparation method comprises the following steps:
[0026] (1) add 150ml methylmonas bacteria liquid and 4ml paraffin oil to 80ml inorganic salt medium, adjust pH value to be 5, stir and mix until there are bubbles on the surface of the solution, then leave standstill until the solution becomes clear; wherein, The inorganic salt culture solution contains solution A and solution B, the volume ratio of the solution A and the solution B is 99:1, and the solution A contains 0.8g / L, 2.5g / L KH 2 PO 4 Na 2 HPO 4 ·12H 2 O, 0.2g / LMgSO 4 ·7H 2 O and 0.8-1.2g / LNH 4 NO 3 , the solution B contains 2.5g / L MgSO 4 ·7H 2 O, 0.4g / L MnSO 4 ·2H 2 O, 0.8g / L NaCl, 0.05g / L FeSO 4 ·7H 2 O, 0.05g / L CaCl 2 ·2H 2 O, 0.05g / L ZnSO 4 ·7H 2 O. 0.01g / L CuSO 4 ·5H 2 O, 0.01g / L KAl (SO 4 ) 2 , 0.01g / L H 3 BO 3 , 0.01g / LNa 2 MoO 4 ·2H 2 O, pH 6.8;
[0027] (2) adding multi-mineral phase particles to the solution obtained in step (1)...
Embodiment 2
[0030] A kind of methane in-situ reducing agent, its preparation method comprises the following steps:
[0031] (1) add 300ml methylobacter bacteria liquid and 9.5ml paraffin oil to 150ml inorganic salt medium, adjust the pH value to be 7, stir and mix until there are bubbles on the surface of the solution, then leave standstill until the solution becomes clear; wherein , the inorganic salt culture solution contains solution A and solution B, the volume ratio of the solution A and solution B is 99:1, and the solution A contains 1.2g / L, 3.0g / LKH 2 PO 4Na 2 HPO 4 ·12H 2 O, 0.4g / LMgSO 4 ·7H 2 O and 1.2g / LNH 4 NO 3 , the solution B contains 3.5g / LMgSO 4 ·7H 2 O. 0.6g / L MnSO 4 ·2H 2 O, 1.2g / L NaCl, 0.15g / L FeSO 4 ·7H 2 O, 0.15g / L CaCl 2 ·2H 2 O, 0.15g / L ZnSO 4 ·7H 2 O, 0.04g / L CuSO 4 ·5H 2 O, 0.02g / L KAl (SO 4 ) 2 , 0.02g / L H 3 BO 3 , 0.02g / L Na 2 MoO 4 ·2H 2 O, pH 7.2;
[0032] (2) adding multi-mineral phase particles to the solution obtained in step (1)...
Embodiment 3
[0035] A kind of methane in-situ reducing agent, its preparation method comprises the following steps:
[0036] (1) add 200ml of Campylobacter methylus bacteria liquid and 7ml of paraffin oil to 120ml of inorganic salt medium, adjust the pH value to be 6, stir and mix until there are air bubbles on the surface of the solution, and then stand until the solution becomes clear; wherein, the inorganic salt culture The liquid contains solution A and solution B, the volume ratio of the solution A and the solution B is 99:1, and the solution A contains 1g / L, 2.8g / L KH 2 PO 4 Na 2 HPO 4 ·12H 2 O, 0.3g / LMgSO 4 ·7H 2 O and 1g / LNH 4 NO 3 , the solution B contains 3g / L MgSO 4 ·7H 2 O, 0.5g / L MnSO 4 ·2H 2 O, 1g / L NaCl, 0.1g / L FeSO 4 ·7H 2 O, 0.1g / L CaCl 2 ·2H 2 O, 0.1g / LZnSO 4 ·7H 2 O, 0.02g / L CuSO 4 ·5H 2 O, 0.01g / L KAl (SO 4 ) 2 , 0.01g / L H 3 BO 3 , 0.01g / LNa 2 MoO 4 ·2H 2 O, pH 7;
[0037] (2) adding multi-mineral phase particles to the solution obtained in st...
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Abstract
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