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Separation device for preparing free flow electrophoresis

A separation device and electrophoresis technology, applied in separation methods, dispersed particle separation, transportation and packaging, etc., can solve the problems of artificial shaking, defoaming and cooling, unevenness, etc., and achieve the effect of improving quality and uniform cooling area.

Inactive Publication Date: 2022-07-22
陈湘杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a separation device for preparing free-flow electrophoresis, which solves the problems of artificial shaking and defoaming and uneven cooling during separation in the existing free-flow electrophoresis

Method used

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  • Separation device for preparing free flow electrophoresis
  • Separation device for preparing free flow electrophoresis
  • Separation device for preparing free flow electrophoresis

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Embodiment

[0026] like Figure 1-5 As shown, the embodiment of the present invention provides a separation device for preparing free flow electrophoresis, including a main fixing mechanism 1, a uniform tiling mechanism 2, a separation mechanism 3 and a tiling cooling mechanism 4, and the top center of the main fixing mechanism 1 is fixedly connected There is a uniform tiling mechanism 2, a separation mechanism 3 is arranged on the top of the uniform tiling mechanism 2, and a tiling cooling mechanism 4 is fixedly connected inside the separation mechanism 3;

[0027] The uniform tiling mechanism 2 includes a fixed frame 201, an externally toothed pulley 202, an internal toothed belt 203, a linkage motor 204, a mosaic block 205 and a sliding column 206. The fixed frame 201 is fixedly connected to the top center of the base frame 101. The two sets of external toothed pulleys 202 are rotatably connected to each other, and the two sets of external toothed pulleys 202 are connected by an intern...

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Abstract

The invention provides a separation device for preparing free flow electrophoresis, and relates to the technical field of biochemical engineering. The separation device for preparing free flow electrophoresis comprises a main fixing mechanism, a uniform spreading mechanism, a separation mechanism and a spreading cooling mechanism, and the uniform spreading mechanism is fixedly connected to the center of the top of the main fixing mechanism. When reaching the operation corner of the internal tooth belt through the embedded block, the embedded block rotates by 180 degrees, the sliding column at the top transversely slides in the driving sliding groove, and then the embedded block drives the driving platform to move in the opposite direction, so that the driving platform reciprocates back and forth, and an electrophoresis buffer solution and an electrode solution in the separation cavity oscillate and swing; the cooling liquid circulates in the circulation pipeline, then the air conveying turbofan is started to generate cooling air, the cooling air is blown into the mixing cavity after being cooled by the circulation pipeline, the cooling area can be uniform through the flatly laid circulation pipeline, and the cooling quality is improved.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a separation device for preparing free flow electrophoresis. Background technique [0002] Continuous free flow electrophoresis is briefly described as an electrophoresis technology that separates and purifies biological macromolecules and cells such as proteins in low ionic strength buffers without solid support media, organic solvents, salts, etc. Compared with gel electrophoresis filled with solid-phase medium, free-flow electrophoresis has the advantages of high efficiency, simple equipment operation, good resolution, controllable process and conditions, and less damage to products. It is the main preparative separation method. At the same time, because free-flow electrophoresis is an all-liquid phase electrophoresis mode, fast separation speed and high sample recovery rate are also its main features. [0003] In the process of electrophoresis separation, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D57/02B01D19/00B01F31/00
CPCB01D57/02B01D19/0078
Inventor 陈湘杰陈襄雨
Owner 陈湘杰
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