Combined marker for deducing individual age of Chinese Han population and application of combined marker

Abstract

The invention discloses a combined marker for deducing the individual age of Chinese Han population and application of the combined marker. The combined marker comprises eight CpG loci, namely, chr6 (chr6): 11044628, cg06639320, cg14361627, chr1: 207823723, cg19283806, cg17740900, cg07553761 and cg26947034, and the CpG loci of the combined marker comprise the following eight CpG loci: the chr6: 11044628, the cg06639320, the cg14361627, the chr1: The eight CpGs disclosed by the invention all have strong age correlation, and the age deduction model constructed by combining the eight CpGs has high accuracy and sensitivity on individual age deduction of Chinese Han people. In actual case application, the age deduction method constructed by the age deduction model can accurately deduce the age of an unknown sample, and the age deduction error is 0.27 years old.

Description

technical field [0001] The present application relates to the technical field of forensic age inference, in particular to a combined marker for inferring the age of an individual of the Chinese Han population and its application. Background technique [0002] With the rapid development of molecular biology, some biomolecular markers associated with human development and aging have been discovered, bringing us new research perspectives and directions. Relevant studies have shown that DNA methylation is significantly superior to other biomolecular markers, such as mRNA, signal-binding T cell receptor deletion loops, aspartic acid racemization, and telomere length, in inferring individual age. It has the advantages of good stability, high accuracy and sensitivity, and is currently recognized as the most promising biomolecular marker. [0003] DNA methylation (CpG) levels at specific sites in the human genome are significantly correlated with age, known as the "epigenetic clock...

AI Technical Summary

Problems solved by technology

However, chip technology is not only costly, but also involves complex bioinformatics analysis. More importantly, it requires more high-quality DNA templates (450K:>500ng; 850K:>250ng), so it cannot be applied well at present. in forensic cases

[0005] In 2015, Huang et al. "Huang Y, Yan J, Hou J, et al.Developing a DNA methylation assay FoR human agepRediction in blood and bloodstain[J]. FoRensic Sci IntGenet.2015, 17:129-136." Selected 6 CpGs at candidate loci (ASPA, ITGA2B, NPTX2, TOM1L1, ZDHHC22, and ZIC4), and a total of 38 loci located within 6 loci in 89 blood samples collected from Han individuals aged 9-75 years were analyzed using pyrosequencing technology. The methylation level of each CpG site was used to construct an age inference model. However, the average absolute error between the inferred age and the actual age was 7.870 years old, and the accuracy was low.

Images