Kit for detecting avian salmonella, clostridium perfringens and escherichia coli

A technology of Clostridium perfringens and Salmonella avian, applied in the field of kits, can solve the problems of mutual mixed infection, long time-consuming, comparative analysis, etc., and achieve the effect of improving sensitivity and test speed

Pending Publication Date: 2022-07-29
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Poultry bacterial diarrhea may occur throughout the year, and it is difficult to distinguish clinically. It can be a single infection or a mixed infection, which has caused great difficulties in the diagnosis of the pathogen.
[0004] The current diagnostic methods for Salmonella avian, Clostridium perfringens and Escherichia coli mainly include bacterial isolation, common PCR and 16s sequencing, etc., but these methods have limitations: bacterial isolation and culture are cumbersome and time-consuming, and common PCR requires Gel electrophoresis analysis, cumbersome operation, relatively low sensitivity, 16s sequencing takes a long time and requires comparative analysis, etc. For this purpose, a kit for detecting avian Salmonella, Clostridium perfringens and Escherichia coli was designed to solve the above problems

Method used

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  • Kit for detecting avian salmonella, clostridium perfringens and escherichia coli
  • Kit for detecting avian salmonella, clostridium perfringens and escherichia coli
  • Kit for detecting avian salmonella, clostridium perfringens and escherichia coli

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Embodiment Construction

[0046] In order to make the technical solutions of the present invention clearer and clearer to those skilled in the art, the present invention will be described in further detail below with reference to the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0047] A kit for detecting Salmonella fowl, Clostridium perfringens and Escherichia coli provided in this example includes PCR Mix, Primer F / R with a concentration of 250nM, and Probe with a concentration of 250nM.

[0048] Carry out the reaction according to the following amplification reaction system (shown in Table 1) and reaction program:

[0049] Table 1

[0050]

[0051] Among them, in the primer-probe mixture, each component is 0.5 μL, and the working concentration is 250 nM;

[0052] The positive standard is pUC57 / InvA-plc-phoA;

[0053] The negative standard is saline without Salmonella, Clostridium perfringens and Escherichia coli nucleic acids....

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Abstract

The invention discloses a kit for detecting avian salmonella, clostridium perfringens and escherichia coli, and belongs to the technical field of kits, the kit comprises PCR Mix, Primer F/R with the concentration of 250 nM and Probe with the concentration of 250 nM. Compared with traditional PCR, the sensitivity and test speed of fluorescent PCR are greatly improved, and multiple pathogens can be specifically detected at the same time.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for detecting Salmonella fowl, Clostridium perfringens and Escherichia coli, and belongs to the technical field of kits. Background technique [0002] Poultry diarrhea disease is one of the main causes of poultry death, which seriously endangers poultry health, affects feed remuneration, and restricts the healthy development of poultry breeding industry; [0003] Among them, Salmonella, Clostridium perfringens and Escherichia coli are the main pathogens causing bacterial diarrhea in poultry. The diarrhea caused by these three kinds of bacteria has similar clinical symptoms. Symptoms such as rough plumage, white or red loose stools, etc., affect the normal growth and development of poultry. Avian bacterial diarrhea may occur all year round, and it is difficult to distinguish clinically. It can be a single infection or a mixed infection, which makes the diagnosis of the pathogen very difficult. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12N15/11C12R1/42C12R1/145C12R1/19
CPCC12Q1/689C12Q1/686C12Q2563/107
Inventor 温肖会吕殿红孙铭飞赵亮翟颀罗胜军贾春玲周秀蓉
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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