Method for improving germination rate of polygonatum kingianum seeds

A technology of Rhizoma Polygonatum and germination rate, which is applied in the field of seedlings of medicinal plants, can solve the problems of increasing operation difficulties and increasing production costs, and achieve the effects of reducing mold rate, reducing production costs, and promoting endosperm metabolism

Pending Publication Date: 2022-08-02
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of hormone treatment in the above patents requires strict control of concentration and time, which increases the difficulty of operation; the use of coating agents and tissue culture will increase production costs

Method used

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  • Method for improving germination rate of polygonatum kingianum seeds
  • Method for improving germination rate of polygonatum kingianum seeds
  • Method for improving germination rate of polygonatum kingianum seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for improving the germination rate of Polygonatum yunnanensis seeds, comprising the following steps:

[0029] S1: Seed Treatment:

[0030] S1.1: The ripe and plump fruits of Polygonatum yunnanensis were placed in a black plastic bag and fermented at 22°C for 12 days;

[0031] S1.2: manual scrubbing to remove the peel and large pieces of pulp to obtain seeds attached to the pulp;

[0032] S1.3: The obtained seeds are enzymatically treated with cellulase and pectinase, the activity of pectinase is 100000u / g, the dosage is 0.1% of the weight of the pure seeds of Dianhuangjing, and the activity of cellulase is 1000u / g g, the dosage is 0.1% of the weight of the pure seeds of Polygonatum yunnanensis, and the specific steps of the enzymatic hydrolysis treatment include: soaking the seeds of Polygonatum yunnanensis after manual cleaning in the enzymatic hydrolysis solution, heat preservation treatment at a temperature of 15 ° C and a pH of 6 24h.

[0033] S1.4: The ...

Embodiment 2

[0039] A method for improving the germination rate of Polygonatum yunnanensis seeds, comprising the following steps:

[0040] S1: Seed Treatment:

[0041] S1.1: The ripe and plump fruits of Polygonati chinensis were placed in a black plastic bag and fermented at 25°C for 15 days;

[0042] S1.2: manual scrubbing to remove the peel and large pieces of pulp to obtain seeds attached to the pulp;

[0043] S1.3: Enzymolysis treatment of the obtained seeds with cellulase and pectinase, the activity of pectinase is 300000u / g, the dosage is 0.05% of the weight of the pure seeds of Dianhuangjing, and the activity of cellulase is 5000u / g g, the dosage is 0.05% of the weight of the pure seeds of Polygonatum yunnanensis, and the specific steps of the enzymatic hydrolysis treatment include: soaking the seeds of Polygonatum yunnanensis after artificial cleaning in the enzymolysis solution, heat preservation treatment at a temperature of 18 ° C and a pH of 6 24h.

[0044] S1.4: The seeds a...

Embodiment 3

[0050] A method for improving the germination rate of Polygonatum yunnanensis seeds, comprising the following steps:

[0051] S1: Seed Treatment:

[0052] S1.1: Dianhuangjing fruit was fermented at 28°C for 18d in a black plastic bag;

[0053] S1.2: manual scrubbing to remove the peel and large pieces of pulp to obtain seeds attached to the pulp;

[0054] S1.3: The obtained seeds are enzymatically treated with cellulase and pectinase, the activity of pectinase is 500000u / g, the dosage is 0.01% of the weight of the pure seeds of Dianhuangjing, and the activity of cellulase is 10000u / g g, the dosage is 0.01% of the weight of the pure seeds of Polygonatum sinensis, and the specific steps of the enzymolysis treatment include: soaking the seeds of Polygonatum sinensis after manual cleaning in the enzymolysis solution, and heat preservation treatment at a temperature of 20 ° C and a pH of 6 24h.

[0055] S1.4: The seeds are rinsed with clean water and gently scrubbed to get clean...

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Abstract

The invention discloses a method for improving the germination rate of polygonatum kingianum seeds. The method comprises the following steps: (1) seed treatment: placing polygonatum kingianum fruits in a black plastic bag for fermentation at 22-28 DEG C for 12-18 days; manually scrubbing to remove peels and large pieces of pulp to obtain seeds attached with the pulp; carrying out enzymolysis treatment on the obtained seeds by using cellulase and pectinase; rinsing the seeds with clear water, and gently scrubbing to obtain clean seeds; placing in a room and airing surface moisture; preserving at low temperature of 4 DEG C; (2) seed germination: gauze is adopted as a germination bed, distilled water is used for fully wetting the germination bed, the germination bed is placed in an illumination incubator, and the humidity of a germination dish is 65-75%; the method is low in cost, the germination rate of the polygonatum kingianum seeds is increased, the mildew rate of the seeds is reduced, the loss of the polygonatum kingianum seeds is reduced, and the method can be popularized and applied in production.

Description

technical field [0001] The invention relates to the field of medicinal plant seeds and seedlings, in particular to a method for improving the germination rate of Polygonatum yunnanensis seeds. Background technique [0002] Polygonatum kingianum (Polygonatum kingianum Coll.et Hemsl.) is a perennial herb of the Liliaceae Polygonatum genus. It is one of the native plants of Polygonatum as a medicinal material stipulated in the 2020 edition of the Pharmacopoeia of the People's Republic of China. It is the key development of Yunnan Province. to medicinal herbs. At present, Dianhuangjing is mainly used for seedling, but the germination rate of seeds is low. In addition, the seeds of Polygonatum yunnanensis are difficult to clean, and a small amount of pulp will be attached to the surface of the seeds, which leads to a high rate of mildew of the seeds of Polygonatum yunnanensis during the stratification process. [0003] At present, the main methods for promoting Polygonatum spp ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00Y02W30/40
Inventor 胡明举杨丽英姚雪娇董志渊石亚娜石瑶丛琨袁理春
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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