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Preparation method of honeysuckle flower extract and application of honeysuckle flower extract in preparation of medicine for preventing or treating scars of burnt or scalded skin

A honeysuckle extract and technology of honeysuckle, applied in the direction of drug combination, skin diseases, medical preparations containing active ingredients, etc., can solve problems such as difficult wound healing

Pending Publication Date: 2022-08-02
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wounds become more difficult to heal with age

Method used

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  • Preparation method of honeysuckle flower extract and application of honeysuckle flower extract in preparation of medicine for preventing or treating scars of burnt or scalded skin
  • Preparation method of honeysuckle flower extract and application of honeysuckle flower extract in preparation of medicine for preventing or treating scars of burnt or scalded skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of Honeysuckle Extract

[0019] Put 5-10 grams of dried honeysuckle in a casserole, add 50-100 ml of ultra-pure water to immerse the honeysuckle in the water, put it on an induction cooker with a power of 1800-2000 watts and boil it for 20-40 minutes, the boiled liquid will pass through 8-16 layers The gauze is coarsely filtered, the liquid is centrifuged at 20-25°C and 5000-8000 rpm for 5-10 minutes, and then the liquid is placed at -80°C for 3-5 hours and placed in a freeze dryer for 1-2 days. Obtain honeysuckle extract.

Embodiment 2

[0021] Animal treatment experiment: The effect of honeysuckle extract on skin healing and scarring of scalded mice, including steps:

[0022] 1) Normal male C57BL / 6 mice aged 6-8 weeks and weighing 20-25 grams were purchased from Jiangsu Jicui Yaokang Biotechnology Co., Ltd., and the mice were randomly divided into 3 groups for feeding. Mice should be acclimated to the environment for at least three days and drink normal sterile water before starting the modeling.

[0023] 2) Create a scald model: shave the normal C57BL / 6J mice that have adapted to the environment, and use depilatory cream to further clear the back hair of the mice, so that the back skin of the mice is exposed, and a round metal rod with a diameter of 2 cm is placed in boiling water. Heat to about 100°C, and quickly place a hot metal rod on the back skin of the mouse for 10 seconds, showing a circular burn mark.

[0024] 3) Wound treatment: On the first day after the establishment of the mouse scald model, ap...

Embodiment 3

[0029] Using hematoxylin-eosin staining method, the skin changes of the mice in Example 2 were observed.

[0030] Hematoxylin-eosin staining can be used to stain tissue for pathological diagnosis. There are thicker epidermis and dermis in scalded skin tissue. The collagen fibers are dense and the collagen bundles are irregularly arranged.

[0031] 1) Cut the injured skin of the mouse back and place it in a 4% paraformaldehyde solution for 12 hours, followed by gradient dehydration: 70% ethanol for 30 minutes → 75% ethanol for 30 minutes → 80% ethanol for 30 minutes → 85% ethanol for 30 minutes × 2 → 90% ethanol for 30 minutes → 95% ethanol for 30 minutes → 100% ethanol for 30 minutes × 2 → ethanol: xylene (1:1) for 30 minutes → xylene for 10-15 minutes → xylene: paraffin (1:1) 30 minutes→paraffin I1h→paraffin II 1h, followed by skin embedding in a small iron box using 75% paraffin.

[0032] 2) Sectioning: Use a paraffin microtome to cut out 5-micron sections of tissue, plac...

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PUM

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Abstract

The invention discloses a preparation method of a honeysuckle flower extract. The preparation method is characterized by comprising the following steps: (1) mixing honeysuckle flowers with water, and boiling; and (2) filtering and centrifugally separating the boiled mixture, and freeze-drying the supernatant to obtain the honeysuckle extract. The invention also discloses application of the honeysuckle flower extract in preparation of medicines for preventing or repairing burn or scalded skin scars. The preparation method disclosed by the invention can be used for stably extracting active ingredients in honeysuckle, and the prepared honeysuckle extract can be used for safely and effectively preventing scars after healing of burns and scalds.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a preparation method of honeysuckle extract and its application in the preparation of a scar medicine for preventing or treating burns or scalded skin. Background technique [0002] In daily life, burns and scalds caused by various reasons can easily cause damage to the skin and physical pain, and the difficulty in repairing skin damage in the future can also easily lead to psychological trauma. And burns, scalds and other injuries, once the dermis is damaged, will produce scars. Scars are mainly divided into superficial scars, hypertrophic scars and pimple scars. Hypertrophic scars are formed after the healing of burns and scalds, that is, the irregular shapes formed by deep wounds on the skin are prominent, and the healing time is longer. The process of scar formation can be divided into three stages: inflammatory stage, proliferative stage, and mature s...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61P17/02A61K133/00
CPCA61K36/355A61P17/02A61K2236/331A61K2236/53A61K2236/51Y02A50/30
Inventor 董磊罗顺煌兴振
Owner NANJING UNIV
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