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Method for rapidly detecting titer of non-starch polysaccharide enzyme for feed

A non-starch polysaccharide enzyme and amylase technology, applied in biochemical equipment and methods, microbial determination/inspection, food processing, etc. The effect of evaluation, simple operation process and accurate measurement results

Pending Publication Date: 2022-08-02
北京盛拓达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the composition of the product to be tested contains amylase, the action of amylase on starch in the feed can also produce reducing sugar, which will interfere with the measurement results and affect the accurate evaluation of the effect of non-starch polysaccharide enzymes on feed

Method used

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  • Method for rapidly detecting titer of non-starch polysaccharide enzyme for feed
  • Method for rapidly detecting titer of non-starch polysaccharide enzyme for feed

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Embodiment 1

[0063] The embodiment of the present application discloses a method for rapidly detecting the titer of non-starch polysaccharide enzymes for feed, comprising the following steps:

[0064] S1, draw the standard curve: first draw the standard curve of the glucose absorbance value and the glucose concentration;

[0065] S2. De-starchization of the feed: After grinding the feed, pass it through a 500 μm aperture screen, take 10 g of the ground feed, add 20 g of water to mix, then add 0.02 g of medium-temperature α-amylase, and put it into an oil bath reactor at 50 ° C The enzymatic hydrolysis reaction was carried out for 3 hours under the condition of enzymatic hydrolysis, and after cooling to room temperature, the pH of the solution after enzymatic hydrolysis by mesophilic α-amylase was adjusted to 3.5, and 0.1 g of saccharification enzyme was added to the shaker and reacted at 50 °C for 4 hours. After 2h, centrifuge (4500rpm, 5min) to get the sediment to obtain the de-starchized...

Embodiment 2

[0078] The embodiment of the present application discloses a method for rapidly detecting the titer of non-starch polysaccharide enzymes for feed, comprising the following steps:

[0079] S1, draw the standard curve: first draw the standard curve of the glucose absorbance value and the glucose concentration;

[0080] S2. De-starchization of feed: After grinding the feed, pass it through a 500μm pore size screen, take 10g of the ground feed, add 50g of water to mix, then add 0.02g of medium-temperature α-amylase, put it in an oil bath reactor at 50°C The enzymatic hydrolysis reaction was carried out for 3 hours under the condition of enzymatic hydrolysis, and after cooling to room temperature, the pH of the solution after enzymatic hydrolysis by mesophilic α-amylase was adjusted to 3.5, and 0.1 g of saccharification enzyme was added to the shaker and reacted at 50 °C for 4 hours. After 2.5h, centrifuge (4500rpm, 5min), take the sediment to obtain the de-starchized feed; S3, in ...

Embodiment 3

[0092] The embodiment of the present application discloses a method for rapidly detecting the titer of non-starch polysaccharide enzymes for feed, comprising the following steps:

[0093] S1, draw the standard curve: first draw the standard curve of the glucose absorbance value and the glucose concentration;

[0094] S2. De-starchization of feed: After grinding the feed, pass it through a sieve with a 500 μm aperture, take 10 g of the ground feed, add 30 g of water to mix, then add 0.02 g of medium-temperature α-amylase, and put it in an oil bath reactor at 50 ° C The enzymatic hydrolysis reaction was carried out for 3 hours under the condition of enzymatic hydrolysis, and after cooling to room temperature, the pH of the solution after enzymatic hydrolysis by mesophilic α-amylase was adjusted to 3.5, and 0.1 g of saccharification enzyme was added to the shaker and reacted at 50 °C for 4 hours. After 3h, centrifuge (4500rpm, 5min) to get the sediment to obtain the de-starchized...

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Abstract

The invention relates to the field of feed compound enzyme quality evaluation, and particularly discloses a method for rapidly detecting the titer of non-starch polysaccharide enzyme for feed, which comprises the following steps: S1, drawing a standard curve: firstly drawing a standard curve of glucose absorbance value and glucose concentration; s2, feed starch removal: mixing the feed and water according to the weight ratio of 1: (2-5), then adding alpha-amylase to react for 3 hours, then adding saccharifying enzyme to react for 4 hours, standing for 2-3 hours, and centrifuging to take precipitate, so as to obtain the starch-removed feed; s3, an in-vitro digestion stage: performing in-vitro enzymolysis digestion on the destarched feed and a to-be-detected enzyme solution to obtain a to-be-detected sample, and determining absorbance of the to-be-detected sample and the blank sample; s4, determining the reducing sugar: calculating the content of the reducing sugar according to the standard curve in the S1 by using the absorbance measured in the S3, and calculating the increment of the reducing sugar according to a formula, and the method has the advantage of improving the accuracy of evaluating the effect of the non-starch polysaccharide enzyme in the feed on the feed.

Description

technical field [0001] The present application relates to the field of quality evaluation of feed compound enzymes, and more particularly, to a method for rapidly detecting the titer of non-starch polysaccharide enzymes used in feed. Background technique [0002] Supplementing enzyme preparations in livestock and poultry diets is a safe and feasible measure. The application of non-starch polysaccharide enzymes in poultry production is more and more extensive. It is one of the most important feed enzymes and is added to various diets. in order to eliminate the anti-nutritional effect of non-starch polysaccharides. As a feed additive, non-starch polysaccharide can hydrolyze non-starch polysaccharides in feed into reducing sugars containing aldehyde groups, which are potentially available nutrients for animals. Therefore, it can significantly improve the nutritional value of feed materials and improve feed conversion. utilization, expanding the application range of feed raw ma...

Claims

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Application Information

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IPC IPC(8): C12Q1/54C12Q1/34C12Q1/40
CPCC12Q1/54C12Q1/34C12Q1/40G01N2333/946G01N2333/924G01N2333/942G01N2333/928Y02P60/87
Inventor 周良娟夏平宇赵邦红黄辉
Owner 北京盛拓达生物技术有限公司
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