Application of PLK1 inhibitor in preparation of medicine for relieving renal fibrosis
A technology for renal fibrosis and renal interstitial fibrosis, which can be used in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., and can solve problems such as lack of effective drugs for chronic kidney disease.
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Embodiment 1
[0035] BI6727 can inhibit the expression of autophagy marker protein LC3-II in kidney tissue of unilateral ureteral ligation (UUO) mice.
[0036] 1. Experimental materials
[0037] The C57 / B6J species mice used in the present invention were purchased from Viton Lever Laboratory Animal Center; BI6727 (Volasertib) was purchased from Selleck Company; corn oil was purchased from Sigma Company; LC3 antibody was purchased from Novus Biologicals.
[0038] 2. Experimental method
[0039] 2.1 Unilateral ureteral obstruction (unilateral ureteral obstruction, UUO) nephropathy model:
[0040]C57 / B6J male mice, aged 8-12 weeks, were randomly divided into 4 groups after adaptive feeding for 1 week: Sham group (Vehicle group, n=6; BI6727 group, n=6), UUO group (Vehicle group, n=6; BI6727 group, n=6). Before the experiment, animals were fed ad libitum and maintained a circadian rhythm of 12 hours of light and 12 hours of darkness. Laboratory temperature: 20-25℃, humidity 50±5%. The exper...
Embodiment 2
[0046] The mRNA expression levels of FN, CollagenI, CollagenIII and α-SMA in the left kidney tissue of mice after unilateral ureteral ligation (UUO) were detected by q-PCR method.
[0047] 1. Experimental materials
[0048] The reverse transcription kit and SYBR green PCR mix used in the present invention were purchased from Vazyme Company; TrizolRNAiso plus was purchased from TAKARA Company; other biological materials and chemical materials were the same as those in Example 1.
[0049] 2. Experimental method
[0050] 2.1 Unilateral ureteral obstruction (unilateral ureteral obstruction, UUO) nephropathy model:
[0051] The method is the same as in Example 1.
[0052] 2.2 Methods of animal administration
[0053] The administration method was the same as that of Example 1, except that kidney tissue was collected and RNA was extracted from the kidney tissue for subsequent detection.
[0054] 3. Experimental results
[0055] The mRNA expression levels of FN, CollagenI, Colla...
Embodiment 3
[0057] Interstitial fibrosis and extracellular matrix deposition in the left mouse kidney one week after unilateral ureteral ligation (UUO) in the mouse kidney.
[0058] 1. Experimental materials
[0059] In the present invention, FN antibody and α-SMA were purchased from Abcam Company, CollagenIII antibody was purchased from Boorson, reagents such as Masson Red and Brilliant Green were purchased from Sinopharm, and other biological materials and chemical materials were the same as those in Example 1.
[0060] 2. Experimental method
[0061] 2.1 Unilateral ureteral obstruction (unilateral ureteral obstruction, UUO) nephropathy model:
[0062] The method is the same as in Example 1.
[0063] 2.2 Methods of animal administration
[0064] The administration method was the same as that in Example 1, except that kidney tissue was collected to prepare paraffin sections, and kidney tissue protein was extracted for subsequent detection.
[0065] 3. Experimental results
[0066] T...
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