Method for immobilizing enzyme on polymer film
A technology of polymer membrane and enzyme immobilization, applied in the direction of immobilization on/in organic carrier, can solve the problems of high cost, small membrane selection range, complicated technology, etc., achieve high stability, increase storage and use period effect
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example 1
[0035] Example 1 uses the SM116 regenerated cellulose membrane produced by SARTORIUS, with a membrane thickness of 110 μm and a pore size of 0.45 μm.
[0036] 1) The membrane is negatively charged by chemical modification, and the reaction occurs on the surface of the hole. To this end, the membrane was soaked in a solution of Ce(IV) dilute sulfuric acid at a concentration of 0.01 mol / L for 15 min at room temperature, and then blotted dry with filter paper. Then soak the membrane in a solution of 0.1 mol / L acrylic acid in 0.05 N sulfuric acid at room temperature for 20 hours, so that the graft polymerization of acrylic acid on the membrane occurs. The modified membrane prepared in this way has very stable properties and can be stored in a dry state or a wet state for a long time at room temperature.
[0037] 2) Soak this film in an aqueous solution of polyvinylimine (10-20g / L) for 20 minutes at room temperature, add a little H to the solution 2 SO 4 Adjust the pH to 10. Th...
example 2
[0040] Example 2: The membrane used is a wet state regenerated cellulose cuproammonia dialysis membrane (produced by ENKA GLANZSTOFF), brand 210PM, and the thickness of the wet membrane is 20 μm.
[0041] The film was treated as described in Example 1, 1) and 2). The method of immobilizing glucose oxidase is also the same as in Example 1.
[0042] The current intensity of the 0.5mM glucose solution measured by an ammeter was 28nA, and the response time was 18 seconds.
example 3
[0043] Example 3: The membrane used is a dialysis membrane made of wet acrylonitrile copolymer (manufactured by RHONE-POULENC), brand AN69, and the thickness of the wet membrane is 40 μm.
[0044] The membrane is already negatively charged and can be used without treatment. The adsorption of polyvinylimine and the immobilization of glucose oxidase were carried out by the same method as described above.
[0045] The current intensity of the 0.5mM glucose solution measured by an ammeter was 22nA, and the response time was 18 seconds.
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