Method for immobilizing enzyme on polymer film

A technology of polymer membrane and enzyme immobilization, applied in the direction of immobilization on/in organic carrier, can solve the problems of high cost, small membrane selection range, complicated technology, etc., achieve high stability, increase storage and use period effect

Inactive Publication Date: 2004-12-22
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these reported methods have many disadvantages, such as technical complexity, high cost, and limited selection of membranes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] Example 1 uses the SM116 regenerated cellulose membrane produced by SARTORIUS, with a membrane thickness of 110 μm and a pore size of 0.45 μm.

[0036] 1) The membrane is negatively charged by chemical modification, and the reaction occurs on the surface of the hole. To this end, the membrane was soaked in a solution of Ce(IV) dilute sulfuric acid at a concentration of 0.01 mol / L for 15 min at room temperature, and then blotted dry with filter paper. Then soak the membrane in a solution of 0.1 mol / L acrylic acid in 0.05 N sulfuric acid at room temperature for 20 hours, so that the graft polymerization of acrylic acid on the membrane occurs. The modified membrane prepared in this way has very stable properties and can be stored in a dry state or a wet state for a long time at room temperature.

[0037] 2) Soak this film in an aqueous solution of polyvinylimine (10-20g / L) for 20 minutes at room temperature, add a little H to the solution 2 SO 4 Adjust the pH to 10. Th...

example 2

[0040] Example 2: The membrane used is a wet state regenerated cellulose cuproammonia dialysis membrane (produced by ENKA GLANZSTOFF), brand 210PM, and the thickness of the wet membrane is 20 μm.

[0041] The film was treated as described in Example 1, 1) and 2). The method of immobilizing glucose oxidase is also the same as in Example 1.

[0042] The current intensity of the 0.5mM glucose solution measured by an ammeter was 28nA, and the response time was 18 seconds.

example 3

[0043] Example 3: The membrane used is a dialysis membrane made of wet acrylonitrile copolymer (manufactured by RHONE-POULENC), brand AN69, and the thickness of the wet membrane is 40 μm.

[0044] The membrane is already negatively charged and can be used without treatment. The adsorption of polyvinylimine and the immobilization of glucose oxidase were carried out by the same method as described above.

[0045] The current intensity of the 0.5mM glucose solution measured by an ammeter was 22nA, and the response time was 18 seconds.

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PUM

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Abstract

In present ivnention,l soaking method is used to make a hydrophilic polymer containing cation or amine group be adsorbed on the polymer membrane containing anion-active centre, then enzyme preparation or other reagent containing separation or catalytic activity are used to make treatment so as to form immobilized enzyme membrane. Said invention utilizes proper crosslinking reaction to raise stability of immobilized membrane. Said invention can adopt similar method to fix the substance with separation and catalytic activity on the polymer membrane to prepare active membrane with separation and synthesis functions.

Description

Technical field: [0001] The present invention relates to a method for the immobilization of enzymes or other components with separation and catalytic activity on different polymer membranes. Background technique: [0002] There have been many reports on methods for immobilizing enzymes on solids, especially on solid particles (I. CHIBATA, Immobilized Enzymes, Chapter 2, p.9-107, Wiley and Sons, 1978). However, it is very difficult to effectively immobilize enzymes on the film, because these film-forming materials must be able to undergo activation reactions, or have good reactivity, or have good adsorption properties for enzymes. [0003] According to literature reports, the membrane materials used to immobilize enzymes are mainly polyamide, cellulose or collagen derivatives. Even if the method of physical adsorption is used to immobilize enzymes, these materials are mainly used. However, these reported methods have many disadvantages, such as technical complexity, high co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 阮光重平郑骅
Owner FUDAN UNIV
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