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Method for micro-propagating tea trees from external leaves plant

A technology for micropropagation and explants, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods, and can solve problems such as failure to obtain morphogenesis.

Inactive Publication Date: 2005-06-15
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is the failure to obtain morphogenetic or amorphous shoot formation
In 1985, Nakamura (Naksmura, Y. "The origin of explants on the root differentiation and its different varieties in tissue culture of tea plants", Shizuoka Tea Experiment Station 62: 1-8; 1985) and Palni, Sood, Chand, Sharma, Rao, and Jain (Palni, L.M.S. Sood, A., Chand, G., Sharma, M., Rao, D.V., Jain, N.K. "Tissue Culture Studies in Tea Trees", Proc.Internat.Sym. "Regarding Tea Science", Shizuoka, Japan, 395-399, 1991) tried the plant regeneration of the leaf explants through the callus phase again, although he obtained rooting from the leaf callus, but failed from this rooting callus to regenerate plants

Method used

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  • Method for micro-propagating tea trees from external leaves plant
  • Method for micro-propagating tea trees from external leaves plant
  • Method for micro-propagating tea trees from external leaves plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] When at 25±2℃ and 52μg / molm -2 the s -1 Reactive explants were cultured in 0.8% agar solidified basic MS supplemented with 3% sucrose and 2.5-10.0 mg / l 2,4-D (pH 5.6 ± 0.2) in the presence of cold fluorescence and under a 16-hour photoperiod of light At 6-10 weeks in culture, in vitro cultured plants of important cultivars (Chinaary, Assamica and Cambod) had fully closed, semi-open or fully opened leaves. Any leaf of the plant was a reactive explant. The callus thus developed after 6-10 weeks was transferred to a medium for rooting or undefined root formation supplemented with 3% sucrose and 0.5-8.0 mg / l 6-benzylaminopurine and 0.1-0.8 mg / l indole -3-butyric acid in 0.8% agar solidified basic MS medium. After 4-10 weeks, the rooted calli were transferred to 0.8% agar-solidified minimal MS medium supplemented with 3% sucrose and 0.5-8.0 mg / l 6-benzylaminopurine for undefined shoot formation. Undefined shoots were excised and placed on 0.8% agar-solidified minimal MS m...

Embodiment 2

[0081] Completely closed, Any leaves of semi-open or fully-open leaf explants were used as explants. The leaves were carefully washed with a black brush and liquid detergent, washed in Tween 20 with (0.1%) carbendazim and (0.05%) streptomycin and chlorinated in 0.01% with one drop of liquid detergent. Surface sterilized in a mercury solution followed by a thorough rinse in distilled water. The sterilized explants were cultured similarly to every detail in the method described above.

Embodiment 3

[0083] When at 25±2℃ and 52μg / molm -2 the s -1 Reactive explants were cultured in 0.8% agar solidified basic MS supplemented with 3% sucrose and 2.5-10.0 mg / l 2,4-D (pH 5.6 ± 0.2) in the presence of cold fluorescence and under a 16-hour photoperiod of light Any leaves from fully closed, semi-open or fully open leaf explants of in vitro cultured plants of other hybrid cultivars such as Tocklai Variety 1 were reactive explants at 6-10 weeks in culture. The callus thus developed after 6-10 weeks was transferred to a medium for rooting or undefined root formation supplemented with 3% sucrose and 0.5-8.0 mg / l 6-benzylaminopurine and 0.1-0.8 mg / l indole- 3-Butyric acid in 0.8% agar solidified with basic MS medium. After 6-10 weeks, the rooted calli were transferred to 0.8% agar-solidified minimal MS medium supplemented with 3% sucrose and 0.5-8.0 mg / l 6-benzylaminopurine for undefined shoot formation. The shoots obtained from the amorphous shoots were propagated for 6 weeks in 20...

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Abstract

The present invention relates to a new method for micropropagation of tea tree plants from explants by culturing the explants in different media, said exosomes consisting of fully closed, semi-open or fully closed Obtained by the open callus phase.

Description

technical field [0001] The present invention relates to an efficient method for micropropagation of tea tree plants (Camelia Sinensis) using explants cut from leaves. Background technique [0002] Tea is a popular caffeinated beverage with anticancer properties (Jankun et al., Why Tea Drinking May Prevent Cancer, Nature 5:561; 1997). Although there are many species of the genus Camellia, only C. sinensis (L.) O. Kuntze or tea and its different cultivars are commercially important (Braua D.N. ed., Science and Practice in Tea Cultivation, Calcutta Tea Research Association; 53-68; 1989). [0003] Tea cultivation is not only an important source of employment but also a major means of earning foreign exchange in all tea-growing regions of the world (Wilson, K.C. "Botany and Plant Improvement" in Coffee, Cocoa and Tea, edited by Wilson, R.C., CABI Publishing, Wallingford, UK: 167-173; 1999). However, the total production of tea leaves is insufficient to meet the needs of the do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 因德拉·桑达尔阿米塔·巴塔查里亚马杜·夏尔马P·S·阿胡贾
Owner COUNCIL OF SCI & IND RES
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