One-step method for producing micro-budlet through tee leaves

A technology for tea leaves and buds, which is applied in the field of one-step method for producing microscopic buds from tea leaves, and can solve problems such as failure to obtain morphogenesis or formation of unshaped buds, and no root formation.

Inactive Publication Date: 2008-07-30
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is the failure to obtain morphogenesis or the formation of amorphous shoots despite the use of different media during the different steps of morphogenesis
[0016] In 1985, Nakamura (Nakamura, Y. The origin of explants on root differentiation in tea plant tissue culture and its effect on different varieties, Shizuoka Tea Experiment Station 62:1-8; 1985) and Palni, Sood, Chand , Sharma, Rao, and Jain (Palni, L.M.S. Sood, A. Chand, G. Sharma, M. Rao, D.V. Jain, N.K. Tissue Culture Studies in Tea, Proc. International Sym. About Tea Science, Shizuoka, Japan, 395- 399, 1991) also tried plant regeneration via callus phase leaf explants, wherein rooting or root formation was obtained from leaf callus, but the disadvantage was that this rooting callus did not produce roots and using two different media

Method used

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  • One-step method for producing micro-budlet through tee leaves
  • One-step method for producing micro-budlet through tee leaves
  • One-step method for producing micro-budlet through tee leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0059] When at 25±2℃ and 52μmolm -2 the s -1 Cold fluorescent 16 hours light will be placed in supplemented with vitamins such as vitamin B 1 -HCl (0.05-2.0mg / l), vitamin B 6-HCl (0.25-1.5mg / ml) and niacin (0.25-1.5mg / l) with glycine (1.0-3.0mg / l) and 2.5-10mg / l of 2,4-dichlorophenoxyacetic acid (pH 5.6 ±0.2) (0.8-1.0%) agar-solidified basic Murashige and Skoog medium (Murashige T. and Skoog F.A. Improved medium for rapid growth and bioassays in tobacco tissue culture, Physiol. Plant 15:473-497; 1962) at 10-16 weeks in in vitro cultured plants of any of the important cultivars (Chinaary, Assamica and Cambod) with fully folded, semi-open or fully expanded leaves. sex explants. 1-2 weeks after the start of the culture, the callus was supplemented with vitamins such as vitamin B 1 -HCl (0.05-2.0mg / l), vitamin B 6 -Basic of HCl (025-1.5mg / ml) and Niacin (0.25-1.5mg / l) with Glycine (1.0-3.0mg / l) and 2,4-dichlorophenoxyacetic acid (2.5-10mg / l) Developed in Murashige and Skoog...

Embodiment 2

[0061] Complete folding with 50-year-old selected plants of any important cultivar (China, Assamica and Cambod) from Himalayan Bioresource Technology Experimental Farm Institute, Banuri, Palampur (36°N and 78.18°E and sea level above 1290m) Leaves from open, semi-open or fully-open leaf explants (second and third leaves from the tip of young shoots) were used as explants. The leaves were washed thoroughly with a brush made of sable hair and liquid detergent, washed in Tween 20 containing (0.1%) carbendazim and (0.05%) streptomycin and washed in 0.01 % mercuric chloride solution followed by a thorough rinse in distilled water. The sterilized explants were cultured similarly to every detail in the method described above.

Embodiment 3

[0063] At 25±2℃ temperature and 52μmolm -2 the s -1 Leaves of fully folded, half-open or fully-open leaf explants of in vitro cultured plants of other hybrid cultivars were placed in a place supplemented with vitamins such as vitamin B 1 -HCl (0.05-2.0mg / l), vitamin B 6 -HCl (0.25-1.5mg / ml) and niacin (0.25-1.5mg / l) with glycine (1.0-3.0mg / l) and 2.5-10mg / l of 2,4-dichlorophenoxyacetic acid (pH 5.6 ±0.2) (0.8-1.0%) agar-solidified basic Murashige and Skoog medium (Murashige T. and Skoog F.A, Improved medium for rapid growth and bioassays in tobacco tissue culture, "Plant Physiology" (Physiol. Plant ) 15:473-497; 1962) in order to develop callus, after 1-2 weeks, take root after 4-6 weeks in the same medium, and shoots after 4-6 weeks. The shoots thus formed were transferred to a propagation medium containing a liquid medium supplemented with 5 μm of phenylthiadiazolyl urea (Sandal I. Bhattacharya A. and Ahuja P.S. 2001, The High-efficiency liquid culture system, plant cell...

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Abstract

The invention provides a method of breeding tea trees from microbody of leaf explantation of callus phase. During the period from induction of callus to taking root and sprouting, putting the explant into basic medium of Murashige and Skoog maintained for 14--16 weeks, said medium is supplemented with vitamine such as vitamine B1-HCI(0.05-2.0 mg / I), vitamine B6-HCI(0.25-1.5 mg / ml), and niacin (0.25-1.5 mg / ml) and glycocoll (1.0-3.0 mg / I) ect.

Description

technical field [0001] The invention relates to a new one-step method for producing micro-sprouts from tea tree leaves. Background technique [0002] Tea is a popular caffeinated beverage with anticancer properties (Jankun et al., Why Tea Drinking May Prevent Cancer, Nature 5:561; 1997). Although there are many species of Camellia, only C. sinensis (L.) O. Kuntze or tea and its different cultivars such as Chinary, Assamica and Cambod are commercially important (Braua D.N. "The Tea Tree Trade" in Barua D.N. ed., Science and Practice in Tea Cultivation, Calcutta Tea Research Association; 53-68; 1989). [0003] Tea cultivation is not only an important source of employment but also a major means of earning foreign exchange in all tea-growing regions of the world ("Botany and Plant Improvement" in Coffee, Cocoa and Tea, edited by Wilson, K.C., CABI Publishing, Wallingford , UK: 167-173; 1999). However, the total production of tea is not enough to meet the needs of the country ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/02
CPCA01H4/005A01H4/008
Inventor 因德拉·桑达尔阿米塔·巴塔查里亚马杜·夏尔马P·S·阿胡贾
Owner COUNCIL OF SCI & IND RES
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