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Eelworm killing biocontrolling bacteria metabolite and production method

A technology for metabolites and nematodes, which is applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of poor solubility and great influence of environmental factors, and achieve high efficacy, strong biological activity, and simplicity cost effect

Inactive Publication Date: 2005-06-15
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It not only overcomes the hazards of chemical pesticide toxicity, but also overcomes the disadvantages of the first generation of nematode biological control agents, which are greatly affected by environmental factors. Poor, difficult to use

Method used

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  • Eelworm killing biocontrolling bacteria metabolite and production method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method for preparing anti-nematode active substances by liquid aerated culture fermentation comprises the following steps:

[0044] With Syncephalastrum racemosum as the strain, the specific preparation process is as follows:

[0045] (1) Cultivation of strains: PSM medium (potato juice of 300g / L of potato sucrose malt extract medium, 20g / L of sucrose, malt extract 2g / L) is used for preservation of bacterial strain slant. Let stand for 6 days and store in a refrigerator at 4°C.

[0046] (2) Preparation of active metabolites:

[0047] Cultivate in shake flasks: Wash the slanted spores with sterile water to make a spore suspension and dilute with 10 6 The spores were inoculated in PSM liquid medium (100 ml in a 500 ml Erlenmeyer flask), with an initial pH of 6.5, and cultured at 26° C. and a shaker speed of 170 rpm for 4 days.

[0048] (3) Dosage form processing: the culture obtained from the above liquid culture is subjected to vacuum filtration to obtain the orig...

Embodiment 2

[0053] Change the liquid medium formula, only be inoculated in the potato sucrose medium (PS medium: the potato juice of 300g / L, the sucrose of 20g / L), other is with embodiment 1, and the biological activity of fermented stoste dilution reaches 85% after doubling. %.

Embodiment 3

[0055] Liquid culture medium changes C / N ratio, the potato juice of 200g / L and the sucrose of 20g / L, initial pH6.7, other are the same as embodiment 2, and the biological activity after the fermentation stoste is diluted one time is 88%.

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Abstract

A metabolite of a nematicidal biocontrol bacterium and a preparation method thereof, relating to a metabolite of Syntocephalus racemosa and its preparation. It solves the problems of the lack of nematode biological control agents and the use of live bacteria agents and fat-soluble agents. The invention is a metabolite of Syncephala racemosa, and is a novel nematode biocontrol agent. Its main active ingredient contains oxalic acid complex with two crystal waters and 8-coordinated potassium, and also tartaric acid, malic acid and lactic acid. The preparation method is to use modern fermentation engineering technology, take Syncephalastrum racemosum as the strain, and mainly use liquid aeration culture to prepare active metabolites. The biocontrol bacterium metabolite has the characteristics of water solubility, favorable preparation processing, strong thermal stability, low cost, wide insecticidal spectrum, easy realization of industrial production, convenient application and the like. The invention has important theoretical significance and broad application prospects for the biological control of nematodes and the research and development of microbial pesticides.

Description

technical field [0001] The invention belongs to a new type of water-soluble nematicide biological preparation and its preparation and application, in particular to a metabolite of Syncephala racemosa and a preparation method thereof. Background technique [0002] Plant parasitic nematodes are one of the important pathogens of plant diseases. Due to their wide parasitic range and strong environmental adaptability, almost every crop is attacked by nematodes. As the damage of nematodes is becoming more and more serious, it poses a serious threat to crop production. The damage caused by parasitic nematodes has caused global crop losses as high as 100 billion US dollars (Sasser & Freckman, 1987), accounting for 2 / 3 of the losses caused by the entire pests and diseases. [0003] my country is located in the temperate zone and subtropical zone, and the climate is suitable for the activity and reproduction of nematodes. Worldwide important plant nematode diseases have occurred in ou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/00
Inventor 孙建华杜连祥宇克莉王建玲张颖彭德良孟庆恒路福平娄威
Owner TIANJIN NORMAL UNIVERSITY
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