Batch production process of biological herbicide
A herbicide and large-volume technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of vitality decline, delay in commercialization process, complicated process, etc., and achieve the effect of uniform growth
Examples
Embodiment 1
[0026] Example 1 Colletotrichum gloeosporioides, a specialized strain QZ-97a (patent application number 00112506.0), whose herbicidal active ingredient is the conidia of the bacterium. The specific implementation method is as follows: the strain QZ-97a is cultured on PDA medium for 7 days, inoculated into corn flour, soybean powder and sucrose liquid medium, cultured for 5-7 days, and crushed with a pulverizer to obtain a bacterial suspension. The solid medium is weighed from wheat bran, soybean powder and sweet potato powder in a dry weight ratio of 7:2:1, and then 1.5 times the weight of the dry material is added with water, and the mixture is mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, and incubate it in a self-made incubator for 3-7 days. The temperature of the room is controlled by air-conditioning at 20°C. The amount of spores produced per gram of dry matter Can reach 7.8×10 9 Pieces / g or more, after sievi...
Embodiment 2
[0037] Example 2 Alternaria eupatorium used crude metabolites to control weeds. The specific implementation method is as follows: Alternaria alternata strain (patent application number 00112560.5) was cultured on PDA medium for 7 days and inoculated into soybean meal and corn meal Cultured in sucrose liquid medium for 7 days, crushed with a pulverizer to obtain a suspension of mycelium. After the solid medium is weighed from the corn flour bean cake according to the dry weight ratio of 1:1, 0.33 times the weight of the dry material is added with water, and the mixture is mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, seal it with a fresh-keeping bag, perforate appropriately, and culture at 25°C for 10 days. After the completion of the culture, the crude toxin was prepared by air-drying. After dilution, the biological activity was tested by acupuncture method, and the toxin production ability was expressed by measur...
Embodiment 3
[0048] Example 3 The herbicidal active ingredient of Crabdosporidium is the conidia of the fungus. The specific implementation method is as follows: strain QZ-2000 (patent application number 011340020.9), cultivated on PDA medium for 5 days, inoculated into corn flour, soybean powder and sucrose liquid medium, cultivated for 5-7 days, pulverized with a pulverizer to obtain hyphae Body suspension. After the solid medium is weighed from soybean flour + cotton seed cake at a dry weight ratio of 2:1, 0.6 times the weight of the dry material is added with water, and mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, incubate in a self-made incubator for 3-8 days, place it under a 20W black light lamp (wavelength 365nm), and irradiate it with a fluorescent lamp for 1-3 The temperature of the room is controlled at 28°C by air-conditioning for a total of 1-10 hours of day or UV light gap irradiation. The maximum spore producti...
PUM
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Description
Claims
Application Information
- IPC
- A01N63/00
- Inventors
- 强胜; 朱秦