Batch production process of biological herbicide

A herbicide and large-volume technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of vitality decline, delay in commercialization process, complicated process, etc., and achieve the effect of uniform growth

Inactive Publication Date: 2005-11-02
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the herbicidal active unit of biological herbicides is often the active part of fungi, such as asexual propagules such as mycelia, conidia, and chlamydospores, and fungal active parts such as conidia produced by liquid fermentation are in the fermentation broth. Preservation, vitality declines faster
For example the biological herbicide Devine  It was the first to be developed as a biological herbicide, and its infestation unit is a fresh suspension of chlamydospores obtained by liquid fermentation, which must be stored like fresh milk, and has a shelf life of only six weeks, leading to its commercialization The process was delayed and was not commercialized until the early 1980s
In addition, in order to obtain high-concentration biological herbicides, a series of steps such as filtration and concentration, dehydration and drying are required. The entire process is complicated and requires special equipment, which increases the cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Colletotrichum gloeosporioides, a specialized strain QZ-97a (patent application number 00112506.0), whose herbicidal active ingredient is the conidia of the bacterium. The specific implementation method is as follows: the strain QZ-97a is cultured on PDA medium for 7 days, inoculated into corn flour, soybean powder and sucrose liquid medium, cultured for 5-7 days, and crushed with a pulverizer to obtain a bacterial suspension. The solid medium is weighed from wheat bran, soybean powder and sweet potato powder in a dry weight ratio of 7:2:1, and then 1.5 times the weight of the dry material is added with water, and the mixture is mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, and incubate it in a self-made incubator for 3-7 days. The temperature of the room is controlled by air-conditioning at 20°C. The amount of spores produced per gram of dry matter Can reach 7.8×10 9 Pieces / g or more, after sievi...

Embodiment 2

[0037] Example 2 Alternaria eupatorium used crude metabolites to control weeds. The specific implementation method is as follows: Alternaria alternata strain (patent application number 00112560.5) was cultured on PDA medium for 7 days and inoculated into soybean meal and corn meal Cultured in sucrose liquid medium for 7 days, crushed with a pulverizer to obtain a suspension of mycelium. After the solid medium is weighed from the corn flour bean cake according to the dry weight ratio of 1:1, 0.33 times the weight of the dry material is added with water, and the mixture is mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, seal it with a fresh-keeping bag, perforate appropriately, and culture at 25°C for 10 days. After the completion of the culture, the crude toxin was prepared by air-drying. After dilution, the biological activity was tested by acupuncture method, and the toxin production ability was expressed by measur...

Embodiment 3

[0048] Example 3 The herbicidal active ingredient of Crabdosporidium is the conidia of the fungus. The specific implementation method is as follows: strain QZ-2000 (patent application number 011340020.9), cultivated on PDA medium for 5 days, inoculated into corn flour, soybean powder and sucrose liquid medium, cultivated for 5-7 days, pulverized with a pulverizer to obtain hyphae Body suspension. After the solid medium is weighed from soybean flour + cotton seed cake at a dry weight ratio of 2:1, 0.6 times the weight of the dry material is added with water, and mixed and sterilized. Inoculate the bacterial suspension on the sterilized solid medium in a sterile environment, incubate in a self-made incubator for 3-8 days, place it under a 20W black light lamp (wavelength 365nm), and irradiate it with a fluorescent lamp for 1-3 The temperature of the room is controlled at 28°C by air-conditioning for a total of 1-10 hours of day or UV light gap irradiation. The maximum spore producti...

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PUM

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Abstract

The present invention belongs to the field of application of microbe in agricultural plant protection. The biological herbicide is batch produced with fungus strain psorosperm anthrax bacteria QZ-97a, purplesteem eupatorium hypnocyst, crab grass hyperbolic spora, common zinnia hypnocyst, etc. and through combined liquid and solid fermentation. During the liquid and solid fermentation, the agricultural product leftover is used as culture medium material, and proper initial temperature is benefit for thallus to obtain nutritive matter and oxygen transmitted and to obtain high conversion rate. Solid fermentation has wider temperature range and easy-to-obtain lighting condition. The process is simple and easy to operate.

Description

1. Technical Field [0001] The invention is a method for mass production of biological herbicides, which belongs to the technical field of application of microorganisms to the protection of agricultural plants and the prevention and control of crop weeds, and is dedicated to the biological control of weeds in farmland. 2. Technical background [0002] At present, the domestic prevention and control of weeds mainly relies on chemical pesticide products, and their use and area are still expanding. With the integration of the global economy and the internationalization of trade, the national pesticide industry is facing severe challenges. The high research and development costs of new varieties of chemical pesticides hinder the development of the pesticide industry and the needs of agricultural production. On the other hand, the extensive use of chemical pesticides has caused and is causing serious environmental pollution problems, directly endangering human and animal health and aff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00
Inventor 强胜朱秦朱云枝徐春凤安传福宋小玲戴宝江蔡建国
Owner NANJING AGRICULTURAL UNIVERSITY
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