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Storage stable liquid formulation comprising a laccase

A liquid preparation and preparation technology, applied in the direction of enzymes, enzyme stabilization, oxidoreductase, etc., can solve the problems of low level of remaining enzyme activity and short storage time

Inactive Publication Date: 2000-04-12
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently available water-based liquid enzyme preparations maintain potency for a relatively short storage time, as residual enzyme activity drops to unacceptably low levels over several weeks if not stored under cooling conditions

Method used

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  • Storage stable liquid formulation comprising a laccase
  • Storage stable liquid formulation comprising a laccase
  • Storage stable liquid formulation comprising a laccase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 5

[0036] Example 5 describes the effect of increasing the pH value from 9 to 10 and is shown in Figure 4 in. Myceliophthora thermophila laccase appears to be more stable at pH 9.0, and after storage at 25°C and 40°C for 4 weeks, the activity still retains 93% and 84%. Mesh porous bacteria laccase preparation

[0037] In addition, as disclosed in WO 96 / 00290 (Novo Nordisk), when syringaldazine is used as a substrate, the optimal pH of the recombinant pore bacteria laccase is 5 to 5.5.

[0038] According to the present invention, the pH of the liquid preparation containing laccase and polyol derived from Porosus sieve is more alkaline than pH 5 to 5.5, preferably about pH 5.5 to 11, especially about pH 6 to 9.5.

[0039] As shown in Example 1, the laccase of the mesh porous bacteria laccase in a 60% PEG6000 liquid with a pH of 9.0, after 14 days at 40°C, the remaining activity is 100%, and after 4 weeks, the remaining activity is 65%. Polyol

[0040] In order to improve the storage sta...

Embodiment 2

[0174] At pH 7.0, 8.0 and 9.0, the storage stability of liquid preparations containing high-purity sieve porous bacteria laccase (10LACU / g) and two different polyols were tested.

[0175] Preparation

[0176] It can be seen from Table 1 and Table 2 that in the presence of polyols, the storage stability is improved. Example 3 Storage stability of sieve porous bacteria laccase (4 weeks)

Embodiment 3

[0177] Preparation

[0178] It can be seen from Table 3 that after 4 weeks, significant residual activity was maintained at a higher pH value. Example 4 Storage stability of Myceliophthora thermophila laccase

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Abstract

The present invention relates to a storage-stable liquid formulation comprising a laccase comprising (i) a laccase, (ii) at least one polyalcohol, which formulation has a pH which is more alkaline than the pH optimum of the laccase. It is also an object of the invention to provide a method for improving the storage-stability of liquid formulations comprising a laccase and the use of said liquid formulations for a personal care application or for bleaching or for textile applications such as dyeing of fabrics.

Description

Invention field [0001] The present invention relates to a shelf-stable liquid formulation containing laccase, a method for improving the storage stability of the liquid formulation containing laccase, and the application of the liquid formulation in personal care, or bleaching, or textiles, for example, for textiles的色。 The staining. Background of the invention [0002] Industrial enzymes are generally formulated as granular solids (such as powder or granular, optionally with some kind of coating), or as water-based solutions. [0003] Many solid preparations (such as enzyme powders) have the disadvantage of being easy to form dust. Unless special precautions are taken, they will pollute the surrounding environment and thereby cause health risks to people who handle these compositions. [0004] Water-based liquid enzyme preparations essentially eliminate the risk of dust formation. Since all enzymes actually exert their activity in the presence of water, it is generally not possib...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N9/96C12R1/645
CPCC12N9/96C12N9/0061
Inventor N·H·索伦森G·拉斯穆森L·R·亨里克森
Owner NOVO NORDISK AS